­­­Kidney organoids recapitulate human basement membrane assembly in health and diseaseCitation formats

  • External authors:
  • Mychel Rpt Morais
  • Pinyuan Tian
  • Craig Lawless
  • Louise Hopkinson
  • David A Long
  • Daniel P Gale
  • Telma Mt Zorn
  • Roy Zent

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­­­Kidney organoids recapitulate human basement membrane assembly in health and disease. / Morais, Mychel Rpt; Tian, Pinyuan; Lawless, Craig; Murtuza-baker, Syed; Hopkinson, Louise; Woods, Steven; Mironov, Aleksandr; Long, David A; Gale, Daniel P; Zorn, Telma Mt; Kimber, Susan J; Zent, Roy; Lennon, Rachel.

In: eLife, Vol. 11, 25.01.2022.

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Morais, Mychel Rpt ; Tian, Pinyuan ; Lawless, Craig ; Murtuza-baker, Syed ; Hopkinson, Louise ; Woods, Steven ; Mironov, Aleksandr ; Long, David A ; Gale, Daniel P ; Zorn, Telma Mt ; Kimber, Susan J ; Zent, Roy ; Lennon, Rachel. / ­­­Kidney organoids recapitulate human basement membrane assembly in health and disease. In: eLife. 2022 ; Vol. 11.

Bibtex

@article{1fe31140ade2442dba1f4d83767f32dc,
title = "­­­Kidney organoids recapitulate human basement membrane assembly in health and disease",
abstract = "Basement membranes (BMs) are complex macromolecular networks underlying all continuous layers of cells. Essential components include collagen IV and laminins, which are affected by human genetic variants leading to a range of debilitating conditions including kidney, muscle, and cerebrovascular phenotypes. We investigated the dynamics of BM assembly in human pluripotent stem cell-derived kidney organoids. We resolved their global BM composition and discovered a conserved temporal sequence in BM assembly that paralleled mammalian fetal kidneys. We identified the emergence of key BM isoforms, which were altered by a pathogenic variant in COL4A5. Integrating organoid, fetal and adult kidney proteomes we found dynamic regulation of BM composition through development to adulthood, and with single-cell transcriptomic analysis we mapped the cellular origins of BM components. Overall, we define the complex and dynamic nature of kidney BM assembly and provide a platform for understanding its wider relevance in human development and disease.",
author = "Morais, {Mychel Rpt} and Pinyuan Tian and Craig Lawless and Syed Murtuza-baker and Louise Hopkinson and Steven Woods and Aleksandr Mironov and Long, {David A} and Gale, {Daniel P} and Zorn, {Telma Mt} and Kimber, {Susan J} and Roy Zent and Rachel Lennon",
year = "2022",
month = jan,
day = "25",
doi = "10.7554/eLife.73486",
language = "English",
volume = "11",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publications",

}

RIS

TY - JOUR

T1 - ­­­Kidney organoids recapitulate human basement membrane assembly in health and disease

AU - Morais, Mychel Rpt

AU - Tian, Pinyuan

AU - Lawless, Craig

AU - Murtuza-baker, Syed

AU - Hopkinson, Louise

AU - Woods, Steven

AU - Mironov, Aleksandr

AU - Long, David A

AU - Gale, Daniel P

AU - Zorn, Telma Mt

AU - Kimber, Susan J

AU - Zent, Roy

AU - Lennon, Rachel

PY - 2022/1/25

Y1 - 2022/1/25

N2 - Basement membranes (BMs) are complex macromolecular networks underlying all continuous layers of cells. Essential components include collagen IV and laminins, which are affected by human genetic variants leading to a range of debilitating conditions including kidney, muscle, and cerebrovascular phenotypes. We investigated the dynamics of BM assembly in human pluripotent stem cell-derived kidney organoids. We resolved their global BM composition and discovered a conserved temporal sequence in BM assembly that paralleled mammalian fetal kidneys. We identified the emergence of key BM isoforms, which were altered by a pathogenic variant in COL4A5. Integrating organoid, fetal and adult kidney proteomes we found dynamic regulation of BM composition through development to adulthood, and with single-cell transcriptomic analysis we mapped the cellular origins of BM components. Overall, we define the complex and dynamic nature of kidney BM assembly and provide a platform for understanding its wider relevance in human development and disease.

AB - Basement membranes (BMs) are complex macromolecular networks underlying all continuous layers of cells. Essential components include collagen IV and laminins, which are affected by human genetic variants leading to a range of debilitating conditions including kidney, muscle, and cerebrovascular phenotypes. We investigated the dynamics of BM assembly in human pluripotent stem cell-derived kidney organoids. We resolved their global BM composition and discovered a conserved temporal sequence in BM assembly that paralleled mammalian fetal kidneys. We identified the emergence of key BM isoforms, which were altered by a pathogenic variant in COL4A5. Integrating organoid, fetal and adult kidney proteomes we found dynamic regulation of BM composition through development to adulthood, and with single-cell transcriptomic analysis we mapped the cellular origins of BM components. Overall, we define the complex and dynamic nature of kidney BM assembly and provide a platform for understanding its wider relevance in human development and disease.

U2 - 10.7554/eLife.73486

DO - 10.7554/eLife.73486

M3 - Article

VL - 11

JO - eLife

JF - eLife

SN - 2050-084X

ER -