Isolation and molecular characterization of polyvinyl chloride (PVC) plastic degrading fungal isolatesCitation formats

  • Authors:
  • Muhammad Ishtiaq Ali
  • Safia Ahmed
  • Geoff Robson
  • Imran Javed
  • Naeem Ali
  • And 2 others
  • External authors:
  • Naima Atiq
  • Abdul Hameed

Standard

Isolation and molecular characterization of polyvinyl chloride (PVC) plastic degrading fungal isolates. / Ali, Muhammad Ishtiaq; Ahmed, Safia; Robson, Geoff; Javed, Imran; Ali, Naeem; Atiq, Naima; Hameed, Abdul.

In: Journal of Basic Microbiology, Vol. 54, No. 1, 01.2014, p. 18-27.

Research output: Contribution to journalArticlepeer-review

Harvard

Ali, MI, Ahmed, S, Robson, G, Javed, I, Ali, N, Atiq, N & Hameed, A 2014, 'Isolation and molecular characterization of polyvinyl chloride (PVC) plastic degrading fungal isolates', Journal of Basic Microbiology, vol. 54, no. 1, pp. 18-27. https://doi.org/10.1002/jobm.201200496

APA

Ali, M. I., Ahmed, S., Robson, G., Javed, I., Ali, N., Atiq, N., & Hameed, A. (2014). Isolation and molecular characterization of polyvinyl chloride (PVC) plastic degrading fungal isolates. Journal of Basic Microbiology, 54(1), 18-27. https://doi.org/10.1002/jobm.201200496

Vancouver

Ali MI, Ahmed S, Robson G, Javed I, Ali N, Atiq N et al. Isolation and molecular characterization of polyvinyl chloride (PVC) plastic degrading fungal isolates. Journal of Basic Microbiology. 2014 Jan;54(1):18-27. https://doi.org/10.1002/jobm.201200496

Author

Ali, Muhammad Ishtiaq ; Ahmed, Safia ; Robson, Geoff ; Javed, Imran ; Ali, Naeem ; Atiq, Naima ; Hameed, Abdul. / Isolation and molecular characterization of polyvinyl chloride (PVC) plastic degrading fungal isolates. In: Journal of Basic Microbiology. 2014 ; Vol. 54, No. 1. pp. 18-27.

Bibtex

@article{8f93b355103a4cab8f8d6d9d9bd98a9a,
title = "Isolation and molecular characterization of polyvinyl chloride (PVC) plastic degrading fungal isolates",
abstract = "The recalcitrant nature of polyvinyl chloride creates serious environmental concerns during manufacturing and waste disposal. The present study was aimed to isolate and screen different soil fungi having potential to biodegrade PVC films. After 10 months of soil burial experiment, it was observed that a number of fungal strains were flourishing on PVC films. On morphological as well as on 18rRNA gene sequence and phylogenetic basis they were identified as Phanerochaete chrysosporium PV1, Lentinus tigrinus PV2, Aspergillus niger PV3, and Aspergillus sydowii PV4. The biodegradation ability of these fungal isolates was further checked in shake flask experiments by taking thin films of PVC (C source) in mineral salt medium. A significant change in color and surface deterioration of PVC films was confirmed through visual observation and Scanning electron microscopy. During shake flask experiments, P. chrysosporium PV1 produced maximum biomass of about 2.57mgml-1 followed by A. niger PV3. P. chrysosporium PV1 showed significant reduction (178,292Da-1) in Molecular weight of the PVC film than control (200,000Da-1) by gel permeation chromatography. Furthermore more Fourier transform infrared spectroscopy and nuclear magnetic resonance also revealed structural changes in the PVC. It was concluded that isolated fungal strains have significant potential for biodegradation of PVC plastics. {\textcopyright} 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.",
keywords = "Biodegradation, Fungi, GPC, Polyvinyl chloride, SEM",
author = "Ali, {Muhammad Ishtiaq} and Safia Ahmed and Geoff Robson and Imran Javed and Naeem Ali and Naima Atiq and Abdul Hameed",
year = "2014",
month = jan,
doi = "10.1002/jobm.201200496",
language = "English",
volume = "54",
pages = "18--27",
journal = "Journal of Basic Microbiology",
issn = "1521-4028",
publisher = "John Wiley & Sons Ltd",
number = "1",

}

RIS

TY - JOUR

T1 - Isolation and molecular characterization of polyvinyl chloride (PVC) plastic degrading fungal isolates

AU - Ali, Muhammad Ishtiaq

AU - Ahmed, Safia

AU - Robson, Geoff

AU - Javed, Imran

AU - Ali, Naeem

AU - Atiq, Naima

AU - Hameed, Abdul

PY - 2014/1

Y1 - 2014/1

N2 - The recalcitrant nature of polyvinyl chloride creates serious environmental concerns during manufacturing and waste disposal. The present study was aimed to isolate and screen different soil fungi having potential to biodegrade PVC films. After 10 months of soil burial experiment, it was observed that a number of fungal strains were flourishing on PVC films. On morphological as well as on 18rRNA gene sequence and phylogenetic basis they were identified as Phanerochaete chrysosporium PV1, Lentinus tigrinus PV2, Aspergillus niger PV3, and Aspergillus sydowii PV4. The biodegradation ability of these fungal isolates was further checked in shake flask experiments by taking thin films of PVC (C source) in mineral salt medium. A significant change in color and surface deterioration of PVC films was confirmed through visual observation and Scanning electron microscopy. During shake flask experiments, P. chrysosporium PV1 produced maximum biomass of about 2.57mgml-1 followed by A. niger PV3. P. chrysosporium PV1 showed significant reduction (178,292Da-1) in Molecular weight of the PVC film than control (200,000Da-1) by gel permeation chromatography. Furthermore more Fourier transform infrared spectroscopy and nuclear magnetic resonance also revealed structural changes in the PVC. It was concluded that isolated fungal strains have significant potential for biodegradation of PVC plastics. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

AB - The recalcitrant nature of polyvinyl chloride creates serious environmental concerns during manufacturing and waste disposal. The present study was aimed to isolate and screen different soil fungi having potential to biodegrade PVC films. After 10 months of soil burial experiment, it was observed that a number of fungal strains were flourishing on PVC films. On morphological as well as on 18rRNA gene sequence and phylogenetic basis they were identified as Phanerochaete chrysosporium PV1, Lentinus tigrinus PV2, Aspergillus niger PV3, and Aspergillus sydowii PV4. The biodegradation ability of these fungal isolates was further checked in shake flask experiments by taking thin films of PVC (C source) in mineral salt medium. A significant change in color and surface deterioration of PVC films was confirmed through visual observation and Scanning electron microscopy. During shake flask experiments, P. chrysosporium PV1 produced maximum biomass of about 2.57mgml-1 followed by A. niger PV3. P. chrysosporium PV1 showed significant reduction (178,292Da-1) in Molecular weight of the PVC film than control (200,000Da-1) by gel permeation chromatography. Furthermore more Fourier transform infrared spectroscopy and nuclear magnetic resonance also revealed structural changes in the PVC. It was concluded that isolated fungal strains have significant potential for biodegradation of PVC plastics. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

KW - Biodegradation

KW - Fungi

KW - GPC

KW - Polyvinyl chloride

KW - SEM

U2 - 10.1002/jobm.201200496

DO - 10.1002/jobm.201200496

M3 - Article

VL - 54

SP - 18

EP - 27

JO - Journal of Basic Microbiology

JF - Journal of Basic Microbiology

SN - 1521-4028

IS - 1

ER -