Invasive oxygen measurements and pimonidazole labeling in human cervix carcinomaCitation formats

  • External authors:
  • Marianne Nordsmark
  • Juliette Loncaster
  • Shu Chuan Chou
  • Hanne Havsteen
  • Jacob C. Lindegaard
  • Susan E. Davidson
  • Mahesh Varia
  • Robin Hunter
  • Jens Overgaard
  • James A. Raleigh

Standard

Invasive oxygen measurements and pimonidazole labeling in human cervix carcinoma. / Nordsmark, Marianne; Loncaster, Juliette; Chou, Shu Chuan; Havsteen, Hanne; Lindegaard, Jacob C.; Davidson, Susan E.; Varia, Mahesh; West, Catharine; Hunter, Robin; Overgaard, Jens; Raleigh, James A.

In: International Journal of Radiation Oncology Biology Physics, Vol. 49, No. 2, 01.02.2001, p. 581-586.

Research output: Contribution to journalArticlepeer-review

Harvard

Nordsmark, M, Loncaster, J, Chou, SC, Havsteen, H, Lindegaard, JC, Davidson, SE, Varia, M, West, C, Hunter, R, Overgaard, J & Raleigh, JA 2001, 'Invasive oxygen measurements and pimonidazole labeling in human cervix carcinoma', International Journal of Radiation Oncology Biology Physics, vol. 49, no. 2, pp. 581-586. https://doi.org/10.1016/S0360-3016(00)01493-0

APA

Nordsmark, M., Loncaster, J., Chou, S. C., Havsteen, H., Lindegaard, J. C., Davidson, S. E., Varia, M., West, C., Hunter, R., Overgaard, J., & Raleigh, J. A. (2001). Invasive oxygen measurements and pimonidazole labeling in human cervix carcinoma. International Journal of Radiation Oncology Biology Physics, 49(2), 581-586. https://doi.org/10.1016/S0360-3016(00)01493-0

Vancouver

Nordsmark M, Loncaster J, Chou SC, Havsteen H, Lindegaard JC, Davidson SE et al. Invasive oxygen measurements and pimonidazole labeling in human cervix carcinoma. International Journal of Radiation Oncology Biology Physics. 2001 Feb 1;49(2):581-586. https://doi.org/10.1016/S0360-3016(00)01493-0

Author

Nordsmark, Marianne ; Loncaster, Juliette ; Chou, Shu Chuan ; Havsteen, Hanne ; Lindegaard, Jacob C. ; Davidson, Susan E. ; Varia, Mahesh ; West, Catharine ; Hunter, Robin ; Overgaard, Jens ; Raleigh, James A. / Invasive oxygen measurements and pimonidazole labeling in human cervix carcinoma. In: International Journal of Radiation Oncology Biology Physics. 2001 ; Vol. 49, No. 2. pp. 581-586.

Bibtex

@article{9b644680d6e44df4a62b89f81236b4f3,
title = "Invasive oxygen measurements and pimonidazole labeling in human cervix carcinoma",
abstract = "Purpose: This study was designed to compare tumor hypoxia assessed by invasive O2 sensitive electrodes and pimonidazole labeling in primary human cervix carcinomas. Methods and Materials: Twenty-eight patients with primary cervix carcinomas (FIGO Stage Ib-IVa) were investigated. Both invasive pO2 measurements and pimonidazole labeling were obtained in all patients. Before treatment, patients were given pimonidazole as a single injection (0.5 g/m2 i.v.). Ten to 24 h later, oxygenation measurements were done by Eppendorf histography, and after this procedure biopsies were taken for pimonidazole-binding analysis. Tumor oxygen partial pressure (pO2) was evaluated as the median tumor pO2 and the fraction of pO2 values ≤ 10 mmHg (HF10). Biopsies were formalin fixed and paraffin embedded, and hypoxia was detected by immunohistochemistry using monoclonal antibodies directed against reductively activated pimonidazole. Pimonidazole binding was evaluated by a semiquantitative scoring system. Results: Both Eppendorf measurements and pimonidazole binding showed large intra-and intertumor variability. A comparison between pimonidazole binding expressed as the fraction of fields at the highest score and HF10 showed a trend for the most well-oxygenated tumors having a low fraction of fields; however, the correlation did not reach statistical significance (p = 0.43, r = 0.165; Spearman's rank correlation test). Conclusion: Hypoxia measured in human uterine cervix carcinomas is heterogeneously expressed both within and between tumors when assessed by either invasive pO2 measurements or pimonidazole binding. Despite a trend that tumors with high pO2 values expressed less pimonidazole binding, no correlation was seen between the two assays in this preliminary report. Copyright {\textcopyright} 2001 Elsevier Science Inc.",
keywords = "Cervix carcinoma, Eppendorf histograph, Hypoxia marker, Pimonidazole, Tumor oxygenation",
author = "Marianne Nordsmark and Juliette Loncaster and Chou, {Shu Chuan} and Hanne Havsteen and Lindegaard, {Jacob C.} and Davidson, {Susan E.} and Mahesh Varia and Catharine West and Robin Hunter and Jens Overgaard and Raleigh, {James A.}",
year = "2001",
month = feb,
day = "1",
doi = "10.1016/S0360-3016(00)01493-0",
language = "English",
volume = "49",
pages = "581--586",
journal = "International Journal of Radiation: Oncology - Biology - Physics",
issn = "0360-3016",
publisher = "Elsevier BV",
number = "2",

}

RIS

TY - JOUR

T1 - Invasive oxygen measurements and pimonidazole labeling in human cervix carcinoma

AU - Nordsmark, Marianne

AU - Loncaster, Juliette

AU - Chou, Shu Chuan

AU - Havsteen, Hanne

AU - Lindegaard, Jacob C.

AU - Davidson, Susan E.

AU - Varia, Mahesh

AU - West, Catharine

AU - Hunter, Robin

AU - Overgaard, Jens

AU - Raleigh, James A.

PY - 2001/2/1

Y1 - 2001/2/1

N2 - Purpose: This study was designed to compare tumor hypoxia assessed by invasive O2 sensitive electrodes and pimonidazole labeling in primary human cervix carcinomas. Methods and Materials: Twenty-eight patients with primary cervix carcinomas (FIGO Stage Ib-IVa) were investigated. Both invasive pO2 measurements and pimonidazole labeling were obtained in all patients. Before treatment, patients were given pimonidazole as a single injection (0.5 g/m2 i.v.). Ten to 24 h later, oxygenation measurements were done by Eppendorf histography, and after this procedure biopsies were taken for pimonidazole-binding analysis. Tumor oxygen partial pressure (pO2) was evaluated as the median tumor pO2 and the fraction of pO2 values ≤ 10 mmHg (HF10). Biopsies were formalin fixed and paraffin embedded, and hypoxia was detected by immunohistochemistry using monoclonal antibodies directed against reductively activated pimonidazole. Pimonidazole binding was evaluated by a semiquantitative scoring system. Results: Both Eppendorf measurements and pimonidazole binding showed large intra-and intertumor variability. A comparison between pimonidazole binding expressed as the fraction of fields at the highest score and HF10 showed a trend for the most well-oxygenated tumors having a low fraction of fields; however, the correlation did not reach statistical significance (p = 0.43, r = 0.165; Spearman's rank correlation test). Conclusion: Hypoxia measured in human uterine cervix carcinomas is heterogeneously expressed both within and between tumors when assessed by either invasive pO2 measurements or pimonidazole binding. Despite a trend that tumors with high pO2 values expressed less pimonidazole binding, no correlation was seen between the two assays in this preliminary report. Copyright © 2001 Elsevier Science Inc.

AB - Purpose: This study was designed to compare tumor hypoxia assessed by invasive O2 sensitive electrodes and pimonidazole labeling in primary human cervix carcinomas. Methods and Materials: Twenty-eight patients with primary cervix carcinomas (FIGO Stage Ib-IVa) were investigated. Both invasive pO2 measurements and pimonidazole labeling were obtained in all patients. Before treatment, patients were given pimonidazole as a single injection (0.5 g/m2 i.v.). Ten to 24 h later, oxygenation measurements were done by Eppendorf histography, and after this procedure biopsies were taken for pimonidazole-binding analysis. Tumor oxygen partial pressure (pO2) was evaluated as the median tumor pO2 and the fraction of pO2 values ≤ 10 mmHg (HF10). Biopsies were formalin fixed and paraffin embedded, and hypoxia was detected by immunohistochemistry using monoclonal antibodies directed against reductively activated pimonidazole. Pimonidazole binding was evaluated by a semiquantitative scoring system. Results: Both Eppendorf measurements and pimonidazole binding showed large intra-and intertumor variability. A comparison between pimonidazole binding expressed as the fraction of fields at the highest score and HF10 showed a trend for the most well-oxygenated tumors having a low fraction of fields; however, the correlation did not reach statistical significance (p = 0.43, r = 0.165; Spearman's rank correlation test). Conclusion: Hypoxia measured in human uterine cervix carcinomas is heterogeneously expressed both within and between tumors when assessed by either invasive pO2 measurements or pimonidazole binding. Despite a trend that tumors with high pO2 values expressed less pimonidazole binding, no correlation was seen between the two assays in this preliminary report. Copyright © 2001 Elsevier Science Inc.

KW - Cervix carcinoma

KW - Eppendorf histograph

KW - Hypoxia marker

KW - Pimonidazole

KW - Tumor oxygenation

U2 - 10.1016/S0360-3016(00)01493-0

DO - 10.1016/S0360-3016(00)01493-0

M3 - Article

VL - 49

SP - 581

EP - 586

JO - International Journal of Radiation: Oncology - Biology - Physics

JF - International Journal of Radiation: Oncology - Biology - Physics

SN - 0360-3016

IS - 2

ER -