Immunological impact of graphene oxide sheets in the abdominal cavity is governed by surface reactivityCitation formats

  • External authors:
  • Leon Newman
  • Isabella Anna Vacchi
  • Cécilia Ménard-Moyon
  • Livia Crica
  • Alberto Bianco

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Immunological impact of graphene oxide sheets in the abdominal cavity is governed by surface reactivity. / Rodrigues, Artur; Newman, Leon; Jasim, Dhifaf; Anna Vacchi, Isabella; Ménard-Moyon, Cécilia; Crica, Livia; Bianco, Alberto; Kostarelos, Kostas (Corresponding); Bussy, Cyrill (Corresponding).

In: Archives of Toxicology, Vol. 92, No. 11, 11.2018, p. 3359-3379.

Research output: Contribution to journalArticle

Harvard

Rodrigues, A, Newman, L, Jasim, D, Anna Vacchi, I, Ménard-Moyon, C, Crica, L, Bianco, A, Kostarelos, K & Bussy, C 2018, 'Immunological impact of graphene oxide sheets in the abdominal cavity is governed by surface reactivity', Archives of Toxicology, vol. 92, no. 11, pp. 3359-3379. https://doi.org/10.1007/s00204-018-2303-z

APA

Vancouver

Rodrigues A, Newman L, Jasim D, Anna Vacchi I, Ménard-Moyon C, Crica L et al. Immunological impact of graphene oxide sheets in the abdominal cavity is governed by surface reactivity. Archives of Toxicology. 2018 Nov;92(11):3359-3379. https://doi.org/10.1007/s00204-018-2303-z

Author

Rodrigues, Artur ; Newman, Leon ; Jasim, Dhifaf ; Anna Vacchi, Isabella ; Ménard-Moyon, Cécilia ; Crica, Livia ; Bianco, Alberto ; Kostarelos, Kostas ; Bussy, Cyrill. / Immunological impact of graphene oxide sheets in the abdominal cavity is governed by surface reactivity. In: Archives of Toxicology. 2018 ; Vol. 92, No. 11. pp. 3359-3379.

Bibtex

@article{42ad539ea5ed402eaa7fe90b7747a5e5,
title = "Immunological impact of graphene oxide sheets in the abdominal cavity is governed by surface reactivity",
abstract = "Graphene oxide (GO) is an oxidised form of graphene that has attractedcommercial interest in multiple applications, including inks, printedelectronics and spray coatings, which all raise health concerns due topotential creation of inhalable aerosols. Although a number of studies havediscussed the toxicity of GO sheets, the invivo impact of their lateral dimensions is still not clear. Here, we compared the effects of large GO sheets (l-GO, 1 µm– 20 µm) with those of small GO sheets (s-GO, < 1 µm) in terms of mesothelialdamage and peritoneal inflammation, after intraperitoneal (i.p.) injection inmice. To benchmark the outcomes, long and rigid multi-walled carbon nanotubes(MWCNTs) that were shown to be associated with asbestos-like pathogenicity onthe mesothelium were also tested. Our aim was to assess whether lateraldimensions can be a predictor of inflammogenicity for GO sheets as good aslength is for MWCNTs.   While long MWCNTs dispersed in 0.5{\%} BSA induced a granulomatous responseon the diaphragmatic mesothelium and immune cell recruitment to the peritonealcavity, GO sheets dispersed under similar conditions did not cause anyresponse, regardless of their lateral dimensions. We further interrogatedwhether tuning the surface reactivity of GO by testing different dispersions(5{\%} dextrose instead of 0.5{\%}BSA) may change the biological outcome. Although the change of dispersion didnot alter the impact of GO on the mesothelium (i.e. no granuloma), weobserved that, when dispersed in protein-free 5{\%} dextrose solution, s-GOelicited a greater recruitment of monocytic cells to the peritoneal cavity thanl-GO, or when dispersed in protein containing solution. Such recruitmentcoincided with the greater ability of s-GO to interact in vivo withperitoneal macrophages and was associated with a greater surface reactivity incomparison to l-GO.In conclusion, large dimension was not a determining factor of the immunological impact of GO sheets afteri.p. administration. For an equal dose, GO sheets with lateral dimensionssimilar to the length of long MWCNTs were less pathogenic than the MWCNTs. Onthe other hand, surface reactivity and the ability of some smaller GO sheets tointeract more readily with immune cells seem to be key parameters that can be tunedto improve the safety profile of GO. In particular, the choice of dispersionmodality, which affected these two parameters, was found to be of crucialimportance in the assessment of GO impact in this model. Overall, thesefindings are essential for a better understanding of the parameters governingGO toxicity and inflammation, and the rational design of safe GO-basedformulations for various applications, including biomedicine.",
keywords = "graphene , toxicity, Intraperitoneal administration, mesothelium, fibrosis, high aspect ratio materials, asbestos, Carbon Nanotubes , Protein coating, Immunotoxicology",
author = "Artur Rodrigues and Leon Newman and Dhifaf Jasim and {Anna Vacchi}, Isabella and C{\'e}cilia M{\'e}nard-Moyon and Livia Crica and Alberto Bianco and Kostas Kostarelos and Cyrill Bussy",
year = "2018",
month = "11",
doi = "10.1007/s00204-018-2303-z",
language = "English",
volume = "92",
pages = "3359--3379",
journal = "Archives of Toxicology",
issn = "0340-5761",
publisher = "Springer Nature",
number = "11",

}

RIS

TY - JOUR

T1 - Immunological impact of graphene oxide sheets in the abdominal cavity is governed by surface reactivity

AU - Rodrigues, Artur

AU - Newman, Leon

AU - Jasim, Dhifaf

AU - Anna Vacchi, Isabella

AU - Ménard-Moyon, Cécilia

AU - Crica, Livia

AU - Bianco, Alberto

A2 - Kostarelos, Kostas

A2 - Bussy, Cyrill

PY - 2018/11

Y1 - 2018/11

N2 - Graphene oxide (GO) is an oxidised form of graphene that has attractedcommercial interest in multiple applications, including inks, printedelectronics and spray coatings, which all raise health concerns due topotential creation of inhalable aerosols. Although a number of studies havediscussed the toxicity of GO sheets, the invivo impact of their lateral dimensions is still not clear. Here, we compared the effects of large GO sheets (l-GO, 1 µm– 20 µm) with those of small GO sheets (s-GO, < 1 µm) in terms of mesothelialdamage and peritoneal inflammation, after intraperitoneal (i.p.) injection inmice. To benchmark the outcomes, long and rigid multi-walled carbon nanotubes(MWCNTs) that were shown to be associated with asbestos-like pathogenicity onthe mesothelium were also tested. Our aim was to assess whether lateraldimensions can be a predictor of inflammogenicity for GO sheets as good aslength is for MWCNTs.   While long MWCNTs dispersed in 0.5% BSA induced a granulomatous responseon the diaphragmatic mesothelium and immune cell recruitment to the peritonealcavity, GO sheets dispersed under similar conditions did not cause anyresponse, regardless of their lateral dimensions. We further interrogatedwhether tuning the surface reactivity of GO by testing different dispersions(5% dextrose instead of 0.5%BSA) may change the biological outcome. Although the change of dispersion didnot alter the impact of GO on the mesothelium (i.e. no granuloma), weobserved that, when dispersed in protein-free 5% dextrose solution, s-GOelicited a greater recruitment of monocytic cells to the peritoneal cavity thanl-GO, or when dispersed in protein containing solution. Such recruitmentcoincided with the greater ability of s-GO to interact in vivo withperitoneal macrophages and was associated with a greater surface reactivity incomparison to l-GO.In conclusion, large dimension was not a determining factor of the immunological impact of GO sheets afteri.p. administration. For an equal dose, GO sheets with lateral dimensionssimilar to the length of long MWCNTs were less pathogenic than the MWCNTs. Onthe other hand, surface reactivity and the ability of some smaller GO sheets tointeract more readily with immune cells seem to be key parameters that can be tunedto improve the safety profile of GO. In particular, the choice of dispersionmodality, which affected these two parameters, was found to be of crucialimportance in the assessment of GO impact in this model. Overall, thesefindings are essential for a better understanding of the parameters governingGO toxicity and inflammation, and the rational design of safe GO-basedformulations for various applications, including biomedicine.

AB - Graphene oxide (GO) is an oxidised form of graphene that has attractedcommercial interest in multiple applications, including inks, printedelectronics and spray coatings, which all raise health concerns due topotential creation of inhalable aerosols. Although a number of studies havediscussed the toxicity of GO sheets, the invivo impact of their lateral dimensions is still not clear. Here, we compared the effects of large GO sheets (l-GO, 1 µm– 20 µm) with those of small GO sheets (s-GO, < 1 µm) in terms of mesothelialdamage and peritoneal inflammation, after intraperitoneal (i.p.) injection inmice. To benchmark the outcomes, long and rigid multi-walled carbon nanotubes(MWCNTs) that were shown to be associated with asbestos-like pathogenicity onthe mesothelium were also tested. Our aim was to assess whether lateraldimensions can be a predictor of inflammogenicity for GO sheets as good aslength is for MWCNTs.   While long MWCNTs dispersed in 0.5% BSA induced a granulomatous responseon the diaphragmatic mesothelium and immune cell recruitment to the peritonealcavity, GO sheets dispersed under similar conditions did not cause anyresponse, regardless of their lateral dimensions. We further interrogatedwhether tuning the surface reactivity of GO by testing different dispersions(5% dextrose instead of 0.5%BSA) may change the biological outcome. Although the change of dispersion didnot alter the impact of GO on the mesothelium (i.e. no granuloma), weobserved that, when dispersed in protein-free 5% dextrose solution, s-GOelicited a greater recruitment of monocytic cells to the peritoneal cavity thanl-GO, or when dispersed in protein containing solution. Such recruitmentcoincided with the greater ability of s-GO to interact in vivo withperitoneal macrophages and was associated with a greater surface reactivity incomparison to l-GO.In conclusion, large dimension was not a determining factor of the immunological impact of GO sheets afteri.p. administration. For an equal dose, GO sheets with lateral dimensionssimilar to the length of long MWCNTs were less pathogenic than the MWCNTs. Onthe other hand, surface reactivity and the ability of some smaller GO sheets tointeract more readily with immune cells seem to be key parameters that can be tunedto improve the safety profile of GO. In particular, the choice of dispersionmodality, which affected these two parameters, was found to be of crucialimportance in the assessment of GO impact in this model. Overall, thesefindings are essential for a better understanding of the parameters governingGO toxicity and inflammation, and the rational design of safe GO-basedformulations for various applications, including biomedicine.

KW - graphene

KW - toxicity

KW - Intraperitoneal administration

KW - mesothelium

KW - fibrosis

KW - high aspect ratio materials

KW - asbestos

KW - Carbon Nanotubes

KW - Protein coating

KW - Immunotoxicology

U2 - 10.1007/s00204-018-2303-z

DO - 10.1007/s00204-018-2303-z

M3 - Article

VL - 92

SP - 3359

EP - 3379

JO - Archives of Toxicology

JF - Archives of Toxicology

SN - 0340-5761

IS - 11

ER -