Imine Reductases, Reductive Aminases, and Amine Oxidases for the Synthesis of Chiral AminesCitation formats

  • External authors:
  • S. C. Cosgrove
  • A. Brzezniak
  • S. P. France
  • J. I. Ramsden
  • J. Mangas-Sanchez
  • S. L. Montgomery
  • R. S. Heath

Standard

Imine Reductases, Reductive Aminases, and Amine Oxidases for the Synthesis of Chiral Amines : Discovery, Characterization, and Synthetic Applications. / Cosgrove, S. C.; Brzezniak, A.; France, S. P.; Ramsden, J. I.; Mangas-Sanchez, J.; Montgomery, S. L.; Heath, R. S.; Turner, N. J.

In: Methods in Enzymology, 01.01.2018.

Research output: Contribution to journalArticle

Harvard

APA

Cosgrove, S. C., Brzezniak, A., France, S. P., Ramsden, J. I., Mangas-Sanchez, J., Montgomery, S. L., ... Turner, N. J. (Accepted/In press). Imine Reductases, Reductive Aminases, and Amine Oxidases for the Synthesis of Chiral Amines: Discovery, Characterization, and Synthetic Applications. Methods in Enzymology. https://doi.org/10.1016/bs.mie.2018.04.022

Vancouver

Author

Cosgrove, S. C. ; Brzezniak, A. ; France, S. P. ; Ramsden, J. I. ; Mangas-Sanchez, J. ; Montgomery, S. L. ; Heath, R. S. ; Turner, N. J. / Imine Reductases, Reductive Aminases, and Amine Oxidases for the Synthesis of Chiral Amines : Discovery, Characterization, and Synthetic Applications. In: Methods in Enzymology. 2018.

Bibtex

@article{d419335b7f314cb28ff5edebeef57d9f,
title = "Imine Reductases, Reductive Aminases, and Amine Oxidases for the Synthesis of Chiral Amines: Discovery, Characterization, and Synthetic Applications",
abstract = "Synthesis of the chiral amine moiety is a key challenge for synthetic organic chemistry due to its prevalence in many biologically active molecules. Imine reductase and amine oxidase enzymes have enabled the biocatalytic synthesis of a host of chiral amine compounds. In this chapter, procedures for the synthesis of chiral amines using imine reductases (IREDs), the recently discovered IRED homologues reductive aminases, and amine oxidases (AOs) are described. Amine oxidases have been the subject of mutagenesis approaches for improvement of substrate scope. The high-throughput screening method for determining active variants in amine oxidase libraries is illustrated. Finally, in an approach which takes inspiration from nature, many enzymes can be combined with each other in cascade reactions. The incorporation of imine reductase and monoamine oxidase biocatalysts into several cascade reactions, both in vitro and in vivo (where the approach moves toward synthetic biology), is reported.",
keywords = "Amine oxidase, Amine oxidase library screening, Biotransformations, Imine reductase, In vivo and in vitro cascade reactions, Reductive aminase",
author = "Cosgrove, {S. C.} and A. Brzezniak and France, {S. P.} and Ramsden, {J. I.} and J. Mangas-Sanchez and Montgomery, {S. L.} and Heath, {R. S.} and Turner, {N. J.}",
year = "2018",
month = "1",
day = "1",
doi = "10.1016/bs.mie.2018.04.022",
language = "English",
journal = "Methods in Enzymology",
issn = "0076-6879",
publisher = "Elsevier BV",

}

RIS

TY - JOUR

T1 - Imine Reductases, Reductive Aminases, and Amine Oxidases for the Synthesis of Chiral Amines

T2 - Discovery, Characterization, and Synthetic Applications

AU - Cosgrove, S. C.

AU - Brzezniak, A.

AU - France, S. P.

AU - Ramsden, J. I.

AU - Mangas-Sanchez, J.

AU - Montgomery, S. L.

AU - Heath, R. S.

AU - Turner, N. J.

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Synthesis of the chiral amine moiety is a key challenge for synthetic organic chemistry due to its prevalence in many biologically active molecules. Imine reductase and amine oxidase enzymes have enabled the biocatalytic synthesis of a host of chiral amine compounds. In this chapter, procedures for the synthesis of chiral amines using imine reductases (IREDs), the recently discovered IRED homologues reductive aminases, and amine oxidases (AOs) are described. Amine oxidases have been the subject of mutagenesis approaches for improvement of substrate scope. The high-throughput screening method for determining active variants in amine oxidase libraries is illustrated. Finally, in an approach which takes inspiration from nature, many enzymes can be combined with each other in cascade reactions. The incorporation of imine reductase and monoamine oxidase biocatalysts into several cascade reactions, both in vitro and in vivo (where the approach moves toward synthetic biology), is reported.

AB - Synthesis of the chiral amine moiety is a key challenge for synthetic organic chemistry due to its prevalence in many biologically active molecules. Imine reductase and amine oxidase enzymes have enabled the biocatalytic synthesis of a host of chiral amine compounds. In this chapter, procedures for the synthesis of chiral amines using imine reductases (IREDs), the recently discovered IRED homologues reductive aminases, and amine oxidases (AOs) are described. Amine oxidases have been the subject of mutagenesis approaches for improvement of substrate scope. The high-throughput screening method for determining active variants in amine oxidase libraries is illustrated. Finally, in an approach which takes inspiration from nature, many enzymes can be combined with each other in cascade reactions. The incorporation of imine reductase and monoamine oxidase biocatalysts into several cascade reactions, both in vitro and in vivo (where the approach moves toward synthetic biology), is reported.

KW - Amine oxidase

KW - Amine oxidase library screening

KW - Biotransformations

KW - Imine reductase

KW - In vivo and in vitro cascade reactions

KW - Reductive aminase

UR - http://www.scopus.com/inward/record.url?scp=85047190237&partnerID=8YFLogxK

U2 - 10.1016/bs.mie.2018.04.022

DO - 10.1016/bs.mie.2018.04.022

M3 - Article

JO - Methods in Enzymology

JF - Methods in Enzymology

SN - 0076-6879

ER -