Human spermbots for patient-representative 3D ovarian cancer cell treatmentCitation formats

  • External authors:
  • Haifeng Xu
  • Mariana Medina-sánchez
  • Wunan Zhang
  • Kang Zeng
  • Steven Bagley
  • Carla Ribeiro
  • Lina P. Restrepo
  • Elkin Lucena
  • Christine K. Schmidt
  • Oliver G. Schmidt

Standard

Human spermbots for patient-representative 3D ovarian cancer cell treatment. / Xu, Haifeng; Medina-sánchez, Mariana; Zhang, Wunan et al.

In: Nanoscale, 23.09.2020.

Research output: Contribution to journalArticlepeer-review

Harvard

Xu, H, Medina-sánchez, M, Zhang, W, Seaton, MPH, Brison, DR, Edmondson, R, Taylor, SS, Nelson, L, Zeng, K, Bagley, S, Ribeiro, C, Restrepo, LP, Lucena, E, Schmidt, CK & Schmidt, OG 2020, 'Human spermbots for patient-representative 3D ovarian cancer cell treatment', Nanoscale. https://doi.org/10.1039/D0NR04488A

APA

Xu, H., Medina-sánchez, M., Zhang, W., Seaton, M. P. H., Brison, D. R., Edmondson, R., Taylor, S. S., Nelson, L., Zeng, K., Bagley, S., Ribeiro, C., Restrepo, L. P., Lucena, E., Schmidt, C. K., & Schmidt, O. G. (2020). Human spermbots for patient-representative 3D ovarian cancer cell treatment. Nanoscale. https://doi.org/10.1039/D0NR04488A

Vancouver

Xu H, Medina-sánchez M, Zhang W, Seaton MPH, Brison DR, Edmondson R et al. Human spermbots for patient-representative 3D ovarian cancer cell treatment. Nanoscale. 2020 Sep 23. Epub 2020 Sep 23. doi: 10.1039/D0NR04488A

Author

Xu, Haifeng ; Medina-sánchez, Mariana ; Zhang, Wunan et al. / Human spermbots for patient-representative 3D ovarian cancer cell treatment. In: Nanoscale. 2020.

Bibtex

@article{c3ee0c9b84954da9b910a2b1f698d4f5,
title = "Human spermbots for patient-representative 3D ovarian cancer cell treatment",
abstract = "Cellular micromotors are attractive for locally delivering high concentrations of drug, and targeting hard-to-reach disease sites such as cervical cancer and early ovarian cancer lesions by non-invasive means. Spermatozoa are highly efficient micromotors perfectly adapted to traveling up the female reproductive system. Indeed, bovine sperm-based micromotors have shown potential to carry drugs toward gynecological cancers. However, due to major differences in the molecular make-up of bovine and human sperm, a key translational bottleneck for bringing this technology closer to the clinic is to transfer this concept to human material. Here, we successfully load human sperm with Doxorubicin (DOX) and perform treatment of 3D cervical cancer and patient-representative ovarian cancer cell cultures, resulting in strong anticancer cell effects. Additionally, we define the subcellular localization of the chemotherapeutic drug within human sperm, using high-resolution optical microscopy. We also assess drug effects on sperm motility and viability over time, employing sperm samples from healty donors as well as assisted reproduction patients. Finally, we demonstrate guidance and release of human drug-loaded sperm onto cancer tissues using magnetic microcaps, and show the sperm microcap loading with a second anticancer drug, camptothecin (CPT), which unlike DOX is not suitable for directly loading into sperm due to its hydrophobic nature. This co-drug delivery approach opens up novel targeted combinatorial drug therapies for future applications.",
author = "Haifeng Xu and Mariana Medina-s{\'a}nchez and Wunan Zhang and Seaton, {Melanie Patricia Hannah} and Brison, {Daniel R.} and Richard Edmondson and Taylor, {Stephen S.} and Louisa Nelson and Kang Zeng and Steven Bagley and Carla Ribeiro and Restrepo, {Lina P.} and Elkin Lucena and Schmidt, {Christine K.} and Schmidt, {Oliver G.}",
year = "2020",
month = sep,
day = "23",
doi = "10.1039/D0NR04488A",
language = "English",
journal = "Nanoscale",
issn = "2040-3372",
publisher = "Royal Society of Chemistry",

}

RIS

TY - JOUR

T1 - Human spermbots for patient-representative 3D ovarian cancer cell treatment

AU - Xu, Haifeng

AU - Medina-sánchez, Mariana

AU - Zhang, Wunan

AU - Seaton, Melanie Patricia Hannah

AU - Brison, Daniel R.

AU - Edmondson, Richard

AU - Taylor, Stephen S.

AU - Nelson, Louisa

AU - Zeng, Kang

AU - Bagley, Steven

AU - Ribeiro, Carla

AU - Restrepo, Lina P.

AU - Lucena, Elkin

AU - Schmidt, Christine K.

AU - Schmidt, Oliver G.

PY - 2020/9/23

Y1 - 2020/9/23

N2 - Cellular micromotors are attractive for locally delivering high concentrations of drug, and targeting hard-to-reach disease sites such as cervical cancer and early ovarian cancer lesions by non-invasive means. Spermatozoa are highly efficient micromotors perfectly adapted to traveling up the female reproductive system. Indeed, bovine sperm-based micromotors have shown potential to carry drugs toward gynecological cancers. However, due to major differences in the molecular make-up of bovine and human sperm, a key translational bottleneck for bringing this technology closer to the clinic is to transfer this concept to human material. Here, we successfully load human sperm with Doxorubicin (DOX) and perform treatment of 3D cervical cancer and patient-representative ovarian cancer cell cultures, resulting in strong anticancer cell effects. Additionally, we define the subcellular localization of the chemotherapeutic drug within human sperm, using high-resolution optical microscopy. We also assess drug effects on sperm motility and viability over time, employing sperm samples from healty donors as well as assisted reproduction patients. Finally, we demonstrate guidance and release of human drug-loaded sperm onto cancer tissues using magnetic microcaps, and show the sperm microcap loading with a second anticancer drug, camptothecin (CPT), which unlike DOX is not suitable for directly loading into sperm due to its hydrophobic nature. This co-drug delivery approach opens up novel targeted combinatorial drug therapies for future applications.

AB - Cellular micromotors are attractive for locally delivering high concentrations of drug, and targeting hard-to-reach disease sites such as cervical cancer and early ovarian cancer lesions by non-invasive means. Spermatozoa are highly efficient micromotors perfectly adapted to traveling up the female reproductive system. Indeed, bovine sperm-based micromotors have shown potential to carry drugs toward gynecological cancers. However, due to major differences in the molecular make-up of bovine and human sperm, a key translational bottleneck for bringing this technology closer to the clinic is to transfer this concept to human material. Here, we successfully load human sperm with Doxorubicin (DOX) and perform treatment of 3D cervical cancer and patient-representative ovarian cancer cell cultures, resulting in strong anticancer cell effects. Additionally, we define the subcellular localization of the chemotherapeutic drug within human sperm, using high-resolution optical microscopy. We also assess drug effects on sperm motility and viability over time, employing sperm samples from healty donors as well as assisted reproduction patients. Finally, we demonstrate guidance and release of human drug-loaded sperm onto cancer tissues using magnetic microcaps, and show the sperm microcap loading with a second anticancer drug, camptothecin (CPT), which unlike DOX is not suitable for directly loading into sperm due to its hydrophobic nature. This co-drug delivery approach opens up novel targeted combinatorial drug therapies for future applications.

U2 - 10.1039/D0NR04488A

DO - 10.1039/D0NR04488A

M3 - Article

JO - Nanoscale

JF - Nanoscale

SN - 2040-3372

ER -