Human monocytes and macrophages regulate immune tolerance via integrin αvβ8–mediated TGFβ activationCitation formats

  • External authors:
  • Aoife Kelly
  • Sezin Gunaltay
  • Craig P. Mcentee
  • Catherine Smedley
  • Stephanie A. Houston
  • Thomas M. Fenton
  • Scott Levison

Standard

Human monocytes and macrophages regulate immune tolerance via integrin αvβ8–mediated TGFβ activation. / Kelly, Aoife; Gunaltay, Sezin; Mcentee, Craig P.; Shuttleworth, Elinor E.; Smedley, Catherine; Houston, Stephanie A.; Fenton, Thomas M.; Levison, Scott; Mann, Elizabeth R.; Travis, Mark A.

In: The Journal of experimental medicine, Vol. 215, No. 11, 05.11.2018.

Research output: Contribution to journalArticle

Harvard

Kelly, A, Gunaltay, S, Mcentee, CP, Shuttleworth, EE, Smedley, C, Houston, SA, Fenton, TM, Levison, S, Mann, ER & Travis, MA 2018, 'Human monocytes and macrophages regulate immune tolerance via integrin αvβ8–mediated TGFβ activation', The Journal of experimental medicine, vol. 215, no. 11. https://doi.org/10.1084/jem.20171491

APA

Kelly, A., Gunaltay, S., Mcentee, C. P., Shuttleworth, E. E., Smedley, C., Houston, S. A., ... Travis, M. A. (2018). Human monocytes and macrophages regulate immune tolerance via integrin αvβ8–mediated TGFβ activation. The Journal of experimental medicine, 215(11). https://doi.org/10.1084/jem.20171491

Vancouver

Kelly A, Gunaltay S, Mcentee CP, Shuttleworth EE, Smedley C, Houston SA et al. Human monocytes and macrophages regulate immune tolerance via integrin αvβ8–mediated TGFβ activation. The Journal of experimental medicine. 2018 Nov 5;215(11). https://doi.org/10.1084/jem.20171491

Author

Kelly, Aoife ; Gunaltay, Sezin ; Mcentee, Craig P. ; Shuttleworth, Elinor E. ; Smedley, Catherine ; Houston, Stephanie A. ; Fenton, Thomas M. ; Levison, Scott ; Mann, Elizabeth R. ; Travis, Mark A. / Human monocytes and macrophages regulate immune tolerance via integrin αvβ8–mediated TGFβ activation. In: The Journal of experimental medicine. 2018 ; Vol. 215, No. 11.

Bibtex

@article{54cc0dfd7f374af786c7b7a58eb7da23,
title = "Human monocytes and macrophages regulate immune tolerance via integrin αvβ8–mediated TGFβ activation",
abstract = "Monocytes are crucial immune cells involved in regulation of inflammation either directly or via differentiation into macrophages in tissues. However, many aspects of how their function is controlled in health and disease are not understood. Here we show that human blood monocytes activate high levels of the cytokine TGFβ, a pathway that is not evident in mouse monocytes. Human CD14+, but not CD16+, monocytes activate TGFβ via expression of the integrin αvβ8 and matrix metalloproteinase 14, which dampens their production of TNFα in response to LPS. Additionally, when monocytes differentiate into macrophages, integrin expression and TGFβ-activating ability are maintained in anti-inflammatory macrophages but down-regulated in pro-inflammatory macrophages. In the healthy human intestine, integrin αvβ8 is highly expressed on mature tissue macrophages, with these cells and their integrin expression being significantly reduced in active inflammatory bowel disease. Thus, our data suggest that integrin αvβ8–mediated TGFβ activation plays a key role in regulation of monocyte inflammatory responses and intestinal macrophage homeostasis.",
author = "Aoife Kelly and Sezin Gunaltay and Mcentee, {Craig P.} and Shuttleworth, {Elinor E.} and Catherine Smedley and Houston, {Stephanie A.} and Fenton, {Thomas M.} and Scott Levison and Mann, {Elizabeth R.} and Travis, {Mark A.}",
year = "2018",
month = "11",
day = "5",
doi = "10.1084/jem.20171491",
language = "English",
volume = "215",
journal = "Journal of Experimental Medicine",
issn = "0022-1007",
publisher = "Rockefeller University Press",
number = "11",

}

RIS

TY - JOUR

T1 - Human monocytes and macrophages regulate immune tolerance via integrin αvβ8–mediated TGFβ activation

AU - Kelly, Aoife

AU - Gunaltay, Sezin

AU - Mcentee, Craig P.

AU - Shuttleworth, Elinor E.

AU - Smedley, Catherine

AU - Houston, Stephanie A.

AU - Fenton, Thomas M.

AU - Levison, Scott

AU - Mann, Elizabeth R.

AU - Travis, Mark A.

PY - 2018/11/5

Y1 - 2018/11/5

N2 - Monocytes are crucial immune cells involved in regulation of inflammation either directly or via differentiation into macrophages in tissues. However, many aspects of how their function is controlled in health and disease are not understood. Here we show that human blood monocytes activate high levels of the cytokine TGFβ, a pathway that is not evident in mouse monocytes. Human CD14+, but not CD16+, monocytes activate TGFβ via expression of the integrin αvβ8 and matrix metalloproteinase 14, which dampens their production of TNFα in response to LPS. Additionally, when monocytes differentiate into macrophages, integrin expression and TGFβ-activating ability are maintained in anti-inflammatory macrophages but down-regulated in pro-inflammatory macrophages. In the healthy human intestine, integrin αvβ8 is highly expressed on mature tissue macrophages, with these cells and their integrin expression being significantly reduced in active inflammatory bowel disease. Thus, our data suggest that integrin αvβ8–mediated TGFβ activation plays a key role in regulation of monocyte inflammatory responses and intestinal macrophage homeostasis.

AB - Monocytes are crucial immune cells involved in regulation of inflammation either directly or via differentiation into macrophages in tissues. However, many aspects of how their function is controlled in health and disease are not understood. Here we show that human blood monocytes activate high levels of the cytokine TGFβ, a pathway that is not evident in mouse monocytes. Human CD14+, but not CD16+, monocytes activate TGFβ via expression of the integrin αvβ8 and matrix metalloproteinase 14, which dampens their production of TNFα in response to LPS. Additionally, when monocytes differentiate into macrophages, integrin expression and TGFβ-activating ability are maintained in anti-inflammatory macrophages but down-regulated in pro-inflammatory macrophages. In the healthy human intestine, integrin αvβ8 is highly expressed on mature tissue macrophages, with these cells and their integrin expression being significantly reduced in active inflammatory bowel disease. Thus, our data suggest that integrin αvβ8–mediated TGFβ activation plays a key role in regulation of monocyte inflammatory responses and intestinal macrophage homeostasis.

U2 - 10.1084/jem.20171491

DO - 10.1084/jem.20171491

M3 - Article

VL - 215

JO - Journal of Experimental Medicine

JF - Journal of Experimental Medicine

SN - 0022-1007

IS - 11

ER -