Human hair follicle epithelium has an antimicrobial defence system that includes the inducible antimicrobial peptide psoriasin (S100A7) and RNase 7Citation formats

  • External authors:
  • K. Reithmayer
  • K. C. Meyer
  • P. Kleditzsch
  • S. Tiede
  • S. K. Uppalapati
  • R. Gläser
  • J. Harder
  • J. M. Schröder

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Human hair follicle epithelium has an antimicrobial defence system that includes the inducible antimicrobial peptide psoriasin (S100A7) and RNase 7. / Reithmayer, K.; Meyer, K. C.; Kleditzsch, P.; Tiede, S.; Uppalapati, S. K.; Gläser, R.; Harder, J.; Schröder, J. M.; Paus, R.

In: British Journal of Dermatology, Vol. 161, No. 1, 07.2009, p. 78-89.

Research output: Contribution to journalArticle

Harvard

Reithmayer, K, Meyer, KC, Kleditzsch, P, Tiede, S, Uppalapati, SK, Gläser, R, Harder, J, Schröder, JM & Paus, R 2009, 'Human hair follicle epithelium has an antimicrobial defence system that includes the inducible antimicrobial peptide psoriasin (S100A7) and RNase 7', British Journal of Dermatology, vol. 161, no. 1, pp. 78-89. https://doi.org/10.1111/j.1365-2133.2009.09154.x

APA

Reithmayer, K., Meyer, K. C., Kleditzsch, P., Tiede, S., Uppalapati, S. K., Gläser, R., ... Paus, R. (2009). Human hair follicle epithelium has an antimicrobial defence system that includes the inducible antimicrobial peptide psoriasin (S100A7) and RNase 7. British Journal of Dermatology, 161(1), 78-89. https://doi.org/10.1111/j.1365-2133.2009.09154.x

Vancouver

Reithmayer K, Meyer KC, Kleditzsch P, Tiede S, Uppalapati SK, Gläser R et al. Human hair follicle epithelium has an antimicrobial defence system that includes the inducible antimicrobial peptide psoriasin (S100A7) and RNase 7. British Journal of Dermatology. 2009 Jul;161(1):78-89. https://doi.org/10.1111/j.1365-2133.2009.09154.x

Author

Reithmayer, K. ; Meyer, K. C. ; Kleditzsch, P. ; Tiede, S. ; Uppalapati, S. K. ; Gläser, R. ; Harder, J. ; Schröder, J. M. ; Paus, R. / Human hair follicle epithelium has an antimicrobial defence system that includes the inducible antimicrobial peptide psoriasin (S100A7) and RNase 7. In: British Journal of Dermatology. 2009 ; Vol. 161, No. 1. pp. 78-89.

Bibtex

@article{be5ad7a2146446479ed4eb00f51a1ec9,
title = "Human hair follicle epithelium has an antimicrobial defence system that includes the inducible antimicrobial peptide psoriasin (S100A7) and RNase 7",
abstract = "Background Hair follicle (HF) ostia represent a potential port of microbial entry into the skin. However, they rarely show clinical signs of infection. This suggests the presence of local, efficient, antimicrobial defence systems, which may include antimicrobial peptides (AMPs). Objectives We determined the presence and distribution of the major AMPs, RNase 7 and psoriasin (S100A7), in human scalp HFs. We investigated whether HF production of these AMPs was induced by prototypic microbial products and proinflammatory cytokines, i.e. interferon (IFN)-γ. Finally, we examined whether the classical pathways for AMP induction, such as toll-like receptor (TLR)4 and TLR5 expression, are present in human HFs and up-regulated after stimulation with bacterium-associated ligands. Methods Cryosections from fresh or organ-cultured full-thickness normal human scalp skin treated with lipopolysaccharide (LPS), flagellin, protein A, lipoteichoic acid (LTA) or IFN-γ were stained for psoriasin and RNase 7 immunoreactivity (IR) as well as for TLR4 and TLR5. In addition, outer root sheath cell culture and semiquantitative analysis of mRNA expression levels of RNase 7 and psoriasin were performed. Results Specific RNase 7 IR was present throughout the entire HF outer root sheath in situ and in cell culture, whereas psoriasin IR was present only in the most distal compartment and not detectable in cultured ORS cells. Upon treatment with Gram-positive (LTA, protein A) or Gram-negative bacterial (LPS, flagellin) cell wall components, or with the cytokine IFN-γ, the IR of both psoriasin and RNase 7 was modified. TLR4 and TLR5 IR was detected in the normal HF epithelium and were upregulated after treatment with their respective ligand. The mRNA analysis confirmed the immunohistochemistry results. Conclusions This pilot study suggests that normal human scalp HF epithelium possesses a functional antimicrobial defence system, which includes the AMPs RNase 7 and psoriasin, and TLRs, and that these are induced by classical microbial products. {\circledC} 2009 British Association of Dermatologists.",
keywords = "Antimicrobial peptides, Human hair follicle, Psoriasin, S100A7",
author = "K. Reithmayer and Meyer, {K. C.} and P. Kleditzsch and S. Tiede and Uppalapati, {S. K.} and R. Gl{\"a}ser and J. Harder and Schr{\"o}der, {J. M.} and R. Paus",
year = "2009",
month = "7",
doi = "10.1111/j.1365-2133.2009.09154.x",
language = "English",
volume = "161",
pages = "78--89",
journal = "British Journal of Dermatology",
issn = "0007-0963",
publisher = "John Wiley & Sons Ltd",
number = "1",

}

RIS

TY - JOUR

T1 - Human hair follicle epithelium has an antimicrobial defence system that includes the inducible antimicrobial peptide psoriasin (S100A7) and RNase 7

AU - Reithmayer, K.

AU - Meyer, K. C.

AU - Kleditzsch, P.

AU - Tiede, S.

AU - Uppalapati, S. K.

AU - Gläser, R.

AU - Harder, J.

AU - Schröder, J. M.

AU - Paus, R.

PY - 2009/7

Y1 - 2009/7

N2 - Background Hair follicle (HF) ostia represent a potential port of microbial entry into the skin. However, they rarely show clinical signs of infection. This suggests the presence of local, efficient, antimicrobial defence systems, which may include antimicrobial peptides (AMPs). Objectives We determined the presence and distribution of the major AMPs, RNase 7 and psoriasin (S100A7), in human scalp HFs. We investigated whether HF production of these AMPs was induced by prototypic microbial products and proinflammatory cytokines, i.e. interferon (IFN)-γ. Finally, we examined whether the classical pathways for AMP induction, such as toll-like receptor (TLR)4 and TLR5 expression, are present in human HFs and up-regulated after stimulation with bacterium-associated ligands. Methods Cryosections from fresh or organ-cultured full-thickness normal human scalp skin treated with lipopolysaccharide (LPS), flagellin, protein A, lipoteichoic acid (LTA) or IFN-γ were stained for psoriasin and RNase 7 immunoreactivity (IR) as well as for TLR4 and TLR5. In addition, outer root sheath cell culture and semiquantitative analysis of mRNA expression levels of RNase 7 and psoriasin were performed. Results Specific RNase 7 IR was present throughout the entire HF outer root sheath in situ and in cell culture, whereas psoriasin IR was present only in the most distal compartment and not detectable in cultured ORS cells. Upon treatment with Gram-positive (LTA, protein A) or Gram-negative bacterial (LPS, flagellin) cell wall components, or with the cytokine IFN-γ, the IR of both psoriasin and RNase 7 was modified. TLR4 and TLR5 IR was detected in the normal HF epithelium and were upregulated after treatment with their respective ligand. The mRNA analysis confirmed the immunohistochemistry results. Conclusions This pilot study suggests that normal human scalp HF epithelium possesses a functional antimicrobial defence system, which includes the AMPs RNase 7 and psoriasin, and TLRs, and that these are induced by classical microbial products. © 2009 British Association of Dermatologists.

AB - Background Hair follicle (HF) ostia represent a potential port of microbial entry into the skin. However, they rarely show clinical signs of infection. This suggests the presence of local, efficient, antimicrobial defence systems, which may include antimicrobial peptides (AMPs). Objectives We determined the presence and distribution of the major AMPs, RNase 7 and psoriasin (S100A7), in human scalp HFs. We investigated whether HF production of these AMPs was induced by prototypic microbial products and proinflammatory cytokines, i.e. interferon (IFN)-γ. Finally, we examined whether the classical pathways for AMP induction, such as toll-like receptor (TLR)4 and TLR5 expression, are present in human HFs and up-regulated after stimulation with bacterium-associated ligands. Methods Cryosections from fresh or organ-cultured full-thickness normal human scalp skin treated with lipopolysaccharide (LPS), flagellin, protein A, lipoteichoic acid (LTA) or IFN-γ were stained for psoriasin and RNase 7 immunoreactivity (IR) as well as for TLR4 and TLR5. In addition, outer root sheath cell culture and semiquantitative analysis of mRNA expression levels of RNase 7 and psoriasin were performed. Results Specific RNase 7 IR was present throughout the entire HF outer root sheath in situ and in cell culture, whereas psoriasin IR was present only in the most distal compartment and not detectable in cultured ORS cells. Upon treatment with Gram-positive (LTA, protein A) or Gram-negative bacterial (LPS, flagellin) cell wall components, or with the cytokine IFN-γ, the IR of both psoriasin and RNase 7 was modified. TLR4 and TLR5 IR was detected in the normal HF epithelium and were upregulated after treatment with their respective ligand. The mRNA analysis confirmed the immunohistochemistry results. Conclusions This pilot study suggests that normal human scalp HF epithelium possesses a functional antimicrobial defence system, which includes the AMPs RNase 7 and psoriasin, and TLRs, and that these are induced by classical microbial products. © 2009 British Association of Dermatologists.

KW - Antimicrobial peptides

KW - Human hair follicle

KW - Psoriasin

KW - S100A7

U2 - 10.1111/j.1365-2133.2009.09154.x

DO - 10.1111/j.1365-2133.2009.09154.x

M3 - Article

VL - 161

SP - 78

EP - 89

JO - British Journal of Dermatology

JF - British Journal of Dermatology

SN - 0007-0963

IS - 1

ER -