Human feeder cell line for derivation and culture of hESc/hiPScCitation formats

  • External authors:
  • Tristan R McKay
  • Maria V Camarasa
  • Banu Iskender
  • Jinpei Ye
  • Duncan Miller
  • Jayne C Fitzsimmons
  • Daniel Foxler
  • Maureen Mee
  • Tyson V Sharp

Standard

Human feeder cell line for derivation and culture of hESc/hiPSc. / McKay, Tristan R; Camarasa, Maria V; Iskender, Banu; Ye, Jinpei; Bates, Nicola; Miller, Duncan; Fitzsimmons, Jayne C; Foxler, Daniel; Mee, Maureen; Sharp, Tyson V; Aplin, John; Brison, Daniel R; Kimber, Susan J.

In: Stem Cell Research, Vol. 7, No. 2, 09.2011, p. 154-162.

Research output: Contribution to journalArticle

Harvard

McKay, TR, Camarasa, MV, Iskender, B, Ye, J, Bates, N, Miller, D, Fitzsimmons, JC, Foxler, D, Mee, M, Sharp, TV, Aplin, J, Brison, DR & Kimber, SJ 2011, 'Human feeder cell line for derivation and culture of hESc/hiPSc', Stem Cell Research, vol. 7, no. 2, pp. 154-162. https://doi.org/10.1016/j.scr.2011.04.005, https://doi.org/10.1016/j.scr.2011.04.005

APA

McKay, T. R., Camarasa, M. V., Iskender, B., Ye, J., Bates, N., Miller, D., Fitzsimmons, J. C., Foxler, D., Mee, M., Sharp, T. V., Aplin, J., Brison, D. R., & Kimber, S. J. (2011). Human feeder cell line for derivation and culture of hESc/hiPSc. Stem Cell Research, 7(2), 154-162. https://doi.org/10.1016/j.scr.2011.04.005, https://doi.org/10.1016/j.scr.2011.04.005

Vancouver

McKay TR, Camarasa MV, Iskender B, Ye J, Bates N, Miller D et al. Human feeder cell line for derivation and culture of hESc/hiPSc. Stem Cell Research. 2011 Sep;7(2):154-162. https://doi.org/10.1016/j.scr.2011.04.005, https://doi.org/10.1016/j.scr.2011.04.005

Author

McKay, Tristan R ; Camarasa, Maria V ; Iskender, Banu ; Ye, Jinpei ; Bates, Nicola ; Miller, Duncan ; Fitzsimmons, Jayne C ; Foxler, Daniel ; Mee, Maureen ; Sharp, Tyson V ; Aplin, John ; Brison, Daniel R ; Kimber, Susan J. / Human feeder cell line for derivation and culture of hESc/hiPSc. In: Stem Cell Research. 2011 ; Vol. 7, No. 2. pp. 154-162.

Bibtex

@article{5baccd4b88304f8cbd049fbe7d8d09d6,
title = "Human feeder cell line for derivation and culture of hESc/hiPSc",
abstract = "We have generated a human feeder cell line from early second trimester Placental Stromal Fibroblasts (ihPSF) stably over-expressing the polycomb protein BMI-1. These feeder cells retain the ability to maintain human Embryonic Stem cells (hESc) over long-term culture whereas hTERT or BMI-1/hTERT immortalised feeder cell lines do not. ihPSFs were able to support the derivation of a new hESc line in near xenofree (free of non-human animal components) conditions and support continued culture of newly derived hESc and human induced Pluripotent Stem (hiPS) cell lines in complete xenofree conditions necessary for clinical use.",
author = "McKay, {Tristan R} and Camarasa, {Maria V} and Banu Iskender and Jinpei Ye and Nicola Bates and Duncan Miller and Fitzsimmons, {Jayne C} and Daniel Foxler and Maureen Mee and Sharp, {Tyson V} and John Aplin and Brison, {Daniel R} and Kimber, {Susan J}",
note = "Copyright {\textcopyright} 2011 Elsevier B.V. All rights reserved.",
year = "2011",
month = sep
doi = "10.1016/j.scr.2011.04.005",
language = "English",
volume = "7",
pages = "154--162",
journal = "Stem Cell Research",
issn = "1873-5061",
publisher = "Elsevier BV",
number = "2",

}

RIS

TY - JOUR

T1 - Human feeder cell line for derivation and culture of hESc/hiPSc

AU - McKay, Tristan R

AU - Camarasa, Maria V

AU - Iskender, Banu

AU - Ye, Jinpei

AU - Bates, Nicola

AU - Miller, Duncan

AU - Fitzsimmons, Jayne C

AU - Foxler, Daniel

AU - Mee, Maureen

AU - Sharp, Tyson V

AU - Aplin, John

AU - Brison, Daniel R

AU - Kimber, Susan J

N1 - Copyright © 2011 Elsevier B.V. All rights reserved.

PY - 2011/9

Y1 - 2011/9

N2 - We have generated a human feeder cell line from early second trimester Placental Stromal Fibroblasts (ihPSF) stably over-expressing the polycomb protein BMI-1. These feeder cells retain the ability to maintain human Embryonic Stem cells (hESc) over long-term culture whereas hTERT or BMI-1/hTERT immortalised feeder cell lines do not. ihPSFs were able to support the derivation of a new hESc line in near xenofree (free of non-human animal components) conditions and support continued culture of newly derived hESc and human induced Pluripotent Stem (hiPS) cell lines in complete xenofree conditions necessary for clinical use.

AB - We have generated a human feeder cell line from early second trimester Placental Stromal Fibroblasts (ihPSF) stably over-expressing the polycomb protein BMI-1. These feeder cells retain the ability to maintain human Embryonic Stem cells (hESc) over long-term culture whereas hTERT or BMI-1/hTERT immortalised feeder cell lines do not. ihPSFs were able to support the derivation of a new hESc line in near xenofree (free of non-human animal components) conditions and support continued culture of newly derived hESc and human induced Pluripotent Stem (hiPS) cell lines in complete xenofree conditions necessary for clinical use.

U2 - 10.1016/j.scr.2011.04.005

DO - 10.1016/j.scr.2011.04.005

M3 - Article

C2 - 21763623

VL - 7

SP - 154

EP - 162

JO - Stem Cell Research

JF - Stem Cell Research

SN - 1873-5061

IS - 2

ER -