Human feeder cell line for derivation and culture of hESc/hiPSc

Research output: Contribution to journalArticle

  • External authors:
  • Tristan R McKay
  • Maria V Camarasa
  • Banu Iskender
  • Jinpei Ye
  • Duncan Miller
  • Jayne C Fitzsimmons
  • Daniel Foxler
  • Maureen Mee
  • Tyson V Sharp
  • Daniel R Brison

Abstract

We have generated a human feeder cell line from early second trimester Placental Stromal Fibroblasts (ihPSF) stably over-expressing the polycomb protein BMI-1. These feeder cells retain the ability to maintain human Embryonic Stem cells (hESc) over long-term culture whereas hTERT or BMI-1/hTERT immortalised feeder cell lines do not. ihPSFs were able to support the derivation of a new hESc line in near xenofree (free of non-human animal components) conditions and support continued culture of newly derived hESc and human induced Pluripotent Stem (hiPS) cell lines in complete xenofree conditions necessary for clinical use.

Bibliographical metadata

Original languageEnglish
Pages (from-to)154-162
Number of pages9
JournalStem Cell Research
Volume7
Issue number2
DOIs
Publication statusPublished - Sep 2011