High throughput gene replacement in Aspergillus fumigatus

Research output: Contribution to journalArticle

  • External authors:
  • Lauren Dineen
  • Ressa Lebedinec
  • Juliane Macheleidt
  • Thorsten Heinekamp
  • Axel A. Brakhage

Abstract

Aspergillus fumigatus is a human pathogen and the principal etiological
agent of invasive and chronic aspergillosis leading to several hundreds of
thousands of deaths every year. Very few antifungals are available to treat infections caused by A. fumigatus and resistance is developing to those we have. Our understanding of the molecular mechanisms that drive pathogenicity and drug resistance have been hampered by the lack of large mutant collections which limit our ability to perform functional genomics analysis. Here we present a high-throughput gene knockout method that combines a highly reproducible fusion PCR method to enable generation of gene replacement cassettes with a multi-well format transformation procedure. This process can be used to generate96 null mutants within 5 days by a single person at a cost of less than £18 ($24) per mutant and is being employed in our laboratory to generate a barcoded genome-wide knockout library in A. fumigatus.

Bibliographical metadata

Original languageEnglish
JournalCurrent Protocols in Microbiology
Early online date7 Aug 2019
DOIs
Publication statusE-pub ahead of print - 7 Aug 2019