Hierarchical porous silk fibroin/poly(L-lactic acid) fibrous membranes towards vascular scaffoldsCitation formats

  • External authors:
  • Zhongda Chen
  • Luis Larrea
  • Dexin Tang

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Hierarchical porous silk fibroin/poly(L-lactic acid) fibrous membranes towards vascular scaffolds. / Song, Jun; Chen, Zhongda; Larrea, Luis; Tang, Dexin; Meng, Chen; Zhong, Xiangli; Wang, Tao (Corresponding); Li, Jiashen (Corresponding).

In: International Journal of Biological Macromolecules, Vol. 166, 04.11.2020, p. 1111-1120.

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Song J, Chen Z, Larrea L, Tang D, Meng C, Zhong X et al. Hierarchical porous silk fibroin/poly(L-lactic acid) fibrous membranes towards vascular scaffolds. International Journal of Biological Macromolecules. 2020 Nov 4;166:1111-1120. https://doi.org/10.1016/j.ijbiomac.2020.10.266

Author

Song, Jun ; Chen, Zhongda ; Larrea, Luis ; Tang, Dexin ; Meng, Chen ; Zhong, Xiangli ; Wang, Tao ; Li, Jiashen. / Hierarchical porous silk fibroin/poly(L-lactic acid) fibrous membranes towards vascular scaffolds. In: International Journal of Biological Macromolecules. 2020 ; Vol. 166. pp. 1111-1120.

Bibtex

@article{a1e7cb7990f04dd58b76a270c462f8ae,
title = "Hierarchical porous silk fibroin/poly(L-lactic acid) fibrous membranes towards vascular scaffolds",
abstract = "Fibrous membranes played an important role to prepare tubular scaffolds for muscular artery regeneration. In this study, a strategy has been developed to combine silk fibroin (SF) with highly porous electrospun poly(L-lactic acid) (PLLA) fibrous membrane towards vascular scaffolds. After PLLA fibres were electrospun and collected, they were immersed into acetone to generate a porous structure with ultra-high surface area. While the pores on PLLA fibres were fulfilled with SF solution and dried, SF was coated uniformly and tightly on PLLA fibres. A multi-layer tubular structure of the tunica media was simulated by winding and stacking a strip of electrospun fibrous membrane. In vitro viability and morphology studies of A7r5 smooth muscle cells were undertaken for up to 14 days. Because the hydrophilicity of SF/PLLA composite fibres were improved dramatically, it had a positive effect on cell adhesion rate (97%) and proliferation (64.4%). Moreover, good cell morphology was observed via a multiphoton laser confocal microscope on SF/PLLA bioactive materials. These results demonstrated that the hierarchical porous SF/PLLA fibrous membranes are promising off-the-shelf scaffolds for muscular artery regeneration.",
keywords = "Electrospinning, Hierarchical porosity, Poly(L-lactic acid), Silk fibroin, Vascular scaffold",
author = "Jun Song and Zhongda Chen and Luis Larrea and Dexin Tang and Chen Meng and Xiangli Zhong and Tao Wang and Jiashen Li",
note = "Funding Information: The authors would like to express thanks to Dr. John Waters in Department of Earth and Environmental Sciences, The University of Manchester, U. K. for his help on BET characterization and analysis. The authors also acknowledge the support of the Electron Microscopy Center and XRD suite in The University of Manchester. Finally, the authors thank to Corbion Company, The Netherlands, for their supply of PLLA material. Luis Larrea thanks EPSRC & MRC Centre for Doctoral Training (CDT) program in Regenerative Medicine. Publisher Copyright: {\textcopyright} 2020",
year = "2020",
month = nov,
day = "4",
doi = "10.1016/j.ijbiomac.2020.10.266",
language = "English",
volume = "166",
pages = "1111--1120",
journal = "International Journal of Biological Macromolecules",
issn = "0141-8130",
publisher = "Elsevier BV",

}

RIS

TY - JOUR

T1 - Hierarchical porous silk fibroin/poly(L-lactic acid) fibrous membranes towards vascular scaffolds

AU - Song, Jun

AU - Chen, Zhongda

AU - Larrea, Luis

AU - Tang, Dexin

AU - Meng, Chen

AU - Zhong, Xiangli

A2 - Wang, Tao

A2 - Li, Jiashen

N1 - Funding Information: The authors would like to express thanks to Dr. John Waters in Department of Earth and Environmental Sciences, The University of Manchester, U. K. for his help on BET characterization and analysis. The authors also acknowledge the support of the Electron Microscopy Center and XRD suite in The University of Manchester. Finally, the authors thank to Corbion Company, The Netherlands, for their supply of PLLA material. Luis Larrea thanks EPSRC & MRC Centre for Doctoral Training (CDT) program in Regenerative Medicine. Publisher Copyright: © 2020

PY - 2020/11/4

Y1 - 2020/11/4

N2 - Fibrous membranes played an important role to prepare tubular scaffolds for muscular artery regeneration. In this study, a strategy has been developed to combine silk fibroin (SF) with highly porous electrospun poly(L-lactic acid) (PLLA) fibrous membrane towards vascular scaffolds. After PLLA fibres were electrospun and collected, they were immersed into acetone to generate a porous structure with ultra-high surface area. While the pores on PLLA fibres were fulfilled with SF solution and dried, SF was coated uniformly and tightly on PLLA fibres. A multi-layer tubular structure of the tunica media was simulated by winding and stacking a strip of electrospun fibrous membrane. In vitro viability and morphology studies of A7r5 smooth muscle cells were undertaken for up to 14 days. Because the hydrophilicity of SF/PLLA composite fibres were improved dramatically, it had a positive effect on cell adhesion rate (97%) and proliferation (64.4%). Moreover, good cell morphology was observed via a multiphoton laser confocal microscope on SF/PLLA bioactive materials. These results demonstrated that the hierarchical porous SF/PLLA fibrous membranes are promising off-the-shelf scaffolds for muscular artery regeneration.

AB - Fibrous membranes played an important role to prepare tubular scaffolds for muscular artery regeneration. In this study, a strategy has been developed to combine silk fibroin (SF) with highly porous electrospun poly(L-lactic acid) (PLLA) fibrous membrane towards vascular scaffolds. After PLLA fibres were electrospun and collected, they were immersed into acetone to generate a porous structure with ultra-high surface area. While the pores on PLLA fibres were fulfilled with SF solution and dried, SF was coated uniformly and tightly on PLLA fibres. A multi-layer tubular structure of the tunica media was simulated by winding and stacking a strip of electrospun fibrous membrane. In vitro viability and morphology studies of A7r5 smooth muscle cells were undertaken for up to 14 days. Because the hydrophilicity of SF/PLLA composite fibres were improved dramatically, it had a positive effect on cell adhesion rate (97%) and proliferation (64.4%). Moreover, good cell morphology was observed via a multiphoton laser confocal microscope on SF/PLLA bioactive materials. These results demonstrated that the hierarchical porous SF/PLLA fibrous membranes are promising off-the-shelf scaffolds for muscular artery regeneration.

KW - Electrospinning

KW - Hierarchical porosity

KW - Poly(L-lactic acid)

KW - Silk fibroin

KW - Vascular scaffold

U2 - 10.1016/j.ijbiomac.2020.10.266

DO - 10.1016/j.ijbiomac.2020.10.266

M3 - Article

VL - 166

SP - 1111

EP - 1120

JO - International Journal of Biological Macromolecules

JF - International Journal of Biological Macromolecules

SN - 0141-8130

ER -