Heterogeneity of mucus glycoproteins from cystic fibrotic sputum. Are there different families of mucins?

Research output: Contribution to journalArticle

Abstract

High-Mr mucin glycopeptides prepared from sputum of an individual with cystic fibrosis (CF) were studied by ion-exchange h.p.l.c. The glycopeptides were heterogeneous and a number of partially resolved populations were identified. Whole mucins from the gel phase were separated into four fractions by isopycnic density-gradient centrifugation in CsCl, and high-Mr glycopeptides from these fractions were examined by ion-exchange h.p.l.c. The acidic nature of the high-Mr glycopeptides increased with increasing buoyant density of the intact mucins, and a periodate-Schiff (PAS)-rich and an extremely high-iron diamine (HID)-reactive component were present in the lowest and highest density fractions respectively. The various glycopeptide populations were identified in different proportions in mucins from four other individuals with CF. CF sputum thus seems to contain distinct mucin populations containing different oligosaccharide clusters corresponding to these high-Mr glycopeptides.

Bibliographical metadata

Original languageEnglish
Pages (from-to)677-682
Number of pages6
JournalThe Biochemical Journal
Volume276 ( Pt 3)
Publication statusPublished - 15 Jun 1991