Supported liquid membranes (SLMs) containing novel green solvents are proposed as a sustainable alternative separation process in the recovery of biomolecules. In this work, succinic acid has been successfully extracted from model fermentation broths through a stripping phase-facilitated transport mechanism with four different green supported liquid membranes: two eutectic solvents (DL-menthol:OctA and N
4444Cl:OctA), the bio-based solvent eucalyptol and the ionic liquid [C
2N]. A permeability activity-based model that takes into account for the first time solute-phase affinities has been developed using the quantum chemical COSMO-RS method; the model corrects the mass transfer driving force and allows extraction predictions beyond the concentration equilibrium. The best recovery has been achieved experimentally for the eucalyptol-based SLM (concentration factor of 1.4) using an alkaline aqueous solution (0.5 M NaOH) as the stripping phase. A countercurrent cascade extraction process design is proposed, and a graphical method to determine the stage number, interstage concentrations as well as mass transfer area requirements is presented. This new tool, the Permeability Activity-Based Linear Operation (PABLO) method, will substantially enhance the process design of SLMs technology for the biorefinery industry.