Graphene Oxide Substrate Promotes Neurotrophic Factor Secretion and Survival of Human Schwann-Like Adipose Mesenchymal Stromal CellsCitation formats

  • External authors:
  • Steffan Llewellyn
  • Cian Bartlam

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Graphene Oxide Substrate Promotes Neurotrophic Factor Secretion and Survival of Human Schwann-Like Adipose Mesenchymal Stromal Cells. / Llewellyn, Steffan; Faroni, Alessandro; Iliut, Maria; Bartlam, Cian; Vijayaraghavan, Aravind; Reid, Adam.

In: Advanced Biology, 04.03.2021.

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@article{1d4d049c58e041488fe87a00f3d62fd8,
title = "Graphene Oxide Substrate Promotes Neurotrophic Factor Secretion and Survival of Human Schwann-Like Adipose Mesenchymal Stromal Cells",
abstract = "Mesenchymal stromal cells from adipose tissue (AD-MSCs) exhibit favourable clinical traits for autologous transplantation and can develop a {\textquoteleft}Schwann-like{\textquoteright} phenotype (sAD-MSCs) to improve peripheral nerve regeneration, where severe injuries yield insufficient recovery. However, sAD-MSCs regress without biochemical stimulation and detach from conduits under unfavourable transplant conditions, negating their paracrine effects. Graphene-derived materials support AD-MSC attachment, regulating cell adhesion and function through physiochemistry and topography. We report graphene oxide (GO) as a suitable substrate for human sAD-MSCs incubation towards severe peripheral nerve injuries, through evaluating transcriptome changes, neurotrophic factor expression over a 7-day period, and cell viability in apoptotic conditions. Transcriptome changes from GO incubation across four patients were minor compared to biological variance. Nerve growth factor (NGF), brain-derived growth factor (BDNF) and glial-derived growth factor (GDNF) gene expression did not change from sAD-MSCs on GO substrates, but NGF and GDNF protein secretion increased at day 3 and 7. Secretome changes did not improve DRG neuron axon outgrowth or sprouting in conditioned media culture models. Fewer sAD-MSCs detached from GO substrates compared to glass following PBS exposure, which simulated apoptotic conditions. Overall, GO substrates are compatible with sAD-MSC primed for peripheral nerve regeneration strategies and protects cell population in harsh environments. ",
author = "Steffan Llewellyn and Alessandro Faroni and Maria Iliut and Cian Bartlam and Aravind Vijayaraghavan and Adam Reid",
year = "2021",
month = mar,
day = "4",
doi = "10.1002/adbi.202000271",
language = "English",
journal = "Advanced Biology",
issn = "2701-0198",
publisher = "John Wiley & Sons Ltd",

}

RIS

TY - JOUR

T1 - Graphene Oxide Substrate Promotes Neurotrophic Factor Secretion and Survival of Human Schwann-Like Adipose Mesenchymal Stromal Cells

AU - Llewellyn, Steffan

AU - Faroni, Alessandro

AU - Iliut, Maria

AU - Bartlam, Cian

AU - Vijayaraghavan, Aravind

AU - Reid, Adam

PY - 2021/3/4

Y1 - 2021/3/4

N2 - Mesenchymal stromal cells from adipose tissue (AD-MSCs) exhibit favourable clinical traits for autologous transplantation and can develop a ‘Schwann-like’ phenotype (sAD-MSCs) to improve peripheral nerve regeneration, where severe injuries yield insufficient recovery. However, sAD-MSCs regress without biochemical stimulation and detach from conduits under unfavourable transplant conditions, negating their paracrine effects. Graphene-derived materials support AD-MSC attachment, regulating cell adhesion and function through physiochemistry and topography. We report graphene oxide (GO) as a suitable substrate for human sAD-MSCs incubation towards severe peripheral nerve injuries, through evaluating transcriptome changes, neurotrophic factor expression over a 7-day period, and cell viability in apoptotic conditions. Transcriptome changes from GO incubation across four patients were minor compared to biological variance. Nerve growth factor (NGF), brain-derived growth factor (BDNF) and glial-derived growth factor (GDNF) gene expression did not change from sAD-MSCs on GO substrates, but NGF and GDNF protein secretion increased at day 3 and 7. Secretome changes did not improve DRG neuron axon outgrowth or sprouting in conditioned media culture models. Fewer sAD-MSCs detached from GO substrates compared to glass following PBS exposure, which simulated apoptotic conditions. Overall, GO substrates are compatible with sAD-MSC primed for peripheral nerve regeneration strategies and protects cell population in harsh environments.

AB - Mesenchymal stromal cells from adipose tissue (AD-MSCs) exhibit favourable clinical traits for autologous transplantation and can develop a ‘Schwann-like’ phenotype (sAD-MSCs) to improve peripheral nerve regeneration, where severe injuries yield insufficient recovery. However, sAD-MSCs regress without biochemical stimulation and detach from conduits under unfavourable transplant conditions, negating their paracrine effects. Graphene-derived materials support AD-MSC attachment, regulating cell adhesion and function through physiochemistry and topography. We report graphene oxide (GO) as a suitable substrate for human sAD-MSCs incubation towards severe peripheral nerve injuries, through evaluating transcriptome changes, neurotrophic factor expression over a 7-day period, and cell viability in apoptotic conditions. Transcriptome changes from GO incubation across four patients were minor compared to biological variance. Nerve growth factor (NGF), brain-derived growth factor (BDNF) and glial-derived growth factor (GDNF) gene expression did not change from sAD-MSCs on GO substrates, but NGF and GDNF protein secretion increased at day 3 and 7. Secretome changes did not improve DRG neuron axon outgrowth or sprouting in conditioned media culture models. Fewer sAD-MSCs detached from GO substrates compared to glass following PBS exposure, which simulated apoptotic conditions. Overall, GO substrates are compatible with sAD-MSC primed for peripheral nerve regeneration strategies and protects cell population in harsh environments.

U2 - 10.1002/adbi.202000271

DO - 10.1002/adbi.202000271

M3 - Article

JO - Advanced Biology

JF - Advanced Biology

SN - 2701-0198

ER -