Graphene oxide is degraded by neutrophils and the degradation products are non-genotoxicCitation formats

  • External authors:
  • Sourav P. Mukherjee
  • Anda R. Gliga
  • Beatrice Lazzaretto
  • Birgit Brandner
  • Matthew Fielden
  • Carmen Vogt
  • Leon Newman
  • Wenting Shao
  • Philip M. Fournier
  • Muhammet S. Toprak
  • Alexander Star
  • Kunal Bhattacharya
  • Bengt Fadeel

Standard

Graphene oxide is degraded by neutrophils and the degradation products are non-genotoxic. / Mukherjee, Sourav P.; Gliga, Anda R.; Lazzaretto, Beatrice; Brandner, Birgit; Fielden, Matthew; Vogt, Carmen; Newman, Leon; Rodrigues, Artur F.; Shao, Wenting; Fournier, Philip M.; Toprak, Muhammet S.; Star, Alexander; Kostarelos, Kostas; Bhattacharya, Kunal; Fadeel, Bengt.

In: Nanoscale, Vol. 10, No. 3, 2017, p. 1180-1188.

Research output: Contribution to journalArticle

Harvard

Mukherjee, SP, Gliga, AR, Lazzaretto, B, Brandner, B, Fielden, M, Vogt, C, Newman, L, Rodrigues, AF, Shao, W, Fournier, PM, Toprak, MS, Star, A, Kostarelos, K, Bhattacharya, K & Fadeel, B 2017, 'Graphene oxide is degraded by neutrophils and the degradation products are non-genotoxic' Nanoscale, vol. 10, no. 3, pp. 1180-1188. https://doi.org/10.1039/c7nr03552g

APA

Mukherjee, S. P., Gliga, A. R., Lazzaretto, B., Brandner, B., Fielden, M., Vogt, C., ... Fadeel, B. (2017). Graphene oxide is degraded by neutrophils and the degradation products are non-genotoxic. Nanoscale, 10(3), 1180-1188. https://doi.org/10.1039/c7nr03552g

Vancouver

Mukherjee SP, Gliga AR, Lazzaretto B, Brandner B, Fielden M, Vogt C et al. Graphene oxide is degraded by neutrophils and the degradation products are non-genotoxic. Nanoscale. 2017;10(3):1180-1188. https://doi.org/10.1039/c7nr03552g

Author

Mukherjee, Sourav P. ; Gliga, Anda R. ; Lazzaretto, Beatrice ; Brandner, Birgit ; Fielden, Matthew ; Vogt, Carmen ; Newman, Leon ; Rodrigues, Artur F. ; Shao, Wenting ; Fournier, Philip M. ; Toprak, Muhammet S. ; Star, Alexander ; Kostarelos, Kostas ; Bhattacharya, Kunal ; Fadeel, Bengt. / Graphene oxide is degraded by neutrophils and the degradation products are non-genotoxic. In: Nanoscale. 2017 ; Vol. 10, No. 3. pp. 1180-1188.

Bibtex

@article{b66e9e4e11874bc88df242731db719e8,
title = "Graphene oxide is degraded by neutrophils and the degradation products are non-genotoxic",
abstract = "Neutrophils were previously shown to digest oxidized carbon nanotubes through a myeloperoxidase (MPO)-dependent mechanism, and graphene oxide (GO) was found to undergo degradation when incubated with purified MPO, but there are no studies to date showing degradation of GO by neutrophils. Here we produced endotoxin-free GO by a modified Hummers' method and asked whether primary human neutrophils stimulated to produce neutrophil extracellular traps or activated to undergo degranulation are capable of digesting GO. Biodegradation was assessed using a range of techniques including Raman spectroscopy, transmission electron microscopy, atomic force microscopy, and mass spectrometry. GO sheets of differing lateral dimensions were effectively degraded by neutrophils. As the degradation products could have toxicological implications, we also evaluated the impact of degraded GO on the bronchial epithelial cell line BEAS-2B. MPO-degraded GO was found to be non-cytotoxic and did not elicit any DNA damage. Taken together, these studies have shown that neutrophils can digest GO and that the biodegraded GO is non-toxic for human lung cells.",
author = "Mukherjee, {Sourav P.} and Gliga, {Anda R.} and Beatrice Lazzaretto and Birgit Brandner and Matthew Fielden and Carmen Vogt and Leon Newman and Rodrigues, {Artur F.} and Wenting Shao and Fournier, {Philip M.} and Toprak, {Muhammet S.} and Alexander Star and Kostas Kostarelos and Kunal Bhattacharya and Bengt Fadeel",
year = "2017",
doi = "10.1039/c7nr03552g",
language = "English",
volume = "10",
pages = "1180--1188",
journal = "Nanoscale",
issn = "2040-3372",
publisher = "Royal Society of Chemistry",
number = "3",

}

RIS

TY - JOUR

T1 - Graphene oxide is degraded by neutrophils and the degradation products are non-genotoxic

AU - Mukherjee, Sourav P.

AU - Gliga, Anda R.

AU - Lazzaretto, Beatrice

AU - Brandner, Birgit

AU - Fielden, Matthew

AU - Vogt, Carmen

AU - Newman, Leon

AU - Rodrigues, Artur F.

AU - Shao, Wenting

AU - Fournier, Philip M.

AU - Toprak, Muhammet S.

AU - Star, Alexander

AU - Kostarelos, Kostas

AU - Bhattacharya, Kunal

AU - Fadeel, Bengt

PY - 2017

Y1 - 2017

N2 - Neutrophils were previously shown to digest oxidized carbon nanotubes through a myeloperoxidase (MPO)-dependent mechanism, and graphene oxide (GO) was found to undergo degradation when incubated with purified MPO, but there are no studies to date showing degradation of GO by neutrophils. Here we produced endotoxin-free GO by a modified Hummers' method and asked whether primary human neutrophils stimulated to produce neutrophil extracellular traps or activated to undergo degranulation are capable of digesting GO. Biodegradation was assessed using a range of techniques including Raman spectroscopy, transmission electron microscopy, atomic force microscopy, and mass spectrometry. GO sheets of differing lateral dimensions were effectively degraded by neutrophils. As the degradation products could have toxicological implications, we also evaluated the impact of degraded GO on the bronchial epithelial cell line BEAS-2B. MPO-degraded GO was found to be non-cytotoxic and did not elicit any DNA damage. Taken together, these studies have shown that neutrophils can digest GO and that the biodegraded GO is non-toxic for human lung cells.

AB - Neutrophils were previously shown to digest oxidized carbon nanotubes through a myeloperoxidase (MPO)-dependent mechanism, and graphene oxide (GO) was found to undergo degradation when incubated with purified MPO, but there are no studies to date showing degradation of GO by neutrophils. Here we produced endotoxin-free GO by a modified Hummers' method and asked whether primary human neutrophils stimulated to produce neutrophil extracellular traps or activated to undergo degranulation are capable of digesting GO. Biodegradation was assessed using a range of techniques including Raman spectroscopy, transmission electron microscopy, atomic force microscopy, and mass spectrometry. GO sheets of differing lateral dimensions were effectively degraded by neutrophils. As the degradation products could have toxicological implications, we also evaluated the impact of degraded GO on the bronchial epithelial cell line BEAS-2B. MPO-degraded GO was found to be non-cytotoxic and did not elicit any DNA damage. Taken together, these studies have shown that neutrophils can digest GO and that the biodegraded GO is non-toxic for human lung cells.

UR - http://www.scopus.com/inward/record.url?scp=85040917284&partnerID=8YFLogxK

U2 - 10.1039/c7nr03552g

DO - 10.1039/c7nr03552g

M3 - Article

VL - 10

SP - 1180

EP - 1188

JO - Nanoscale

JF - Nanoscale

SN - 2040-3372

IS - 3

ER -