Graphene Oxide Flakes Tune Excitatory Neurotransmission in Vivo by Targeting Hippocampal SynapsesCitation formats

  • External authors:
  • Rossana Rauti
  • Manuela Medelin
  • Leon Newman
  • Giacomo Reina
  • Alberto Bianco
  • Maurizio Prato
  • Laura Ballerini

Standard

Graphene Oxide Flakes Tune Excitatory Neurotransmission in Vivo by Targeting Hippocampal Synapses. / Rauti, Rossana; Medelin, Manuela; Newman, Leon; Vranic, Sandra; Reina, Giacomo; Bianco, Alberto; Prato, Maurizio; Kostarelos, Kostas; Ballerini, Laura.

In: Nano Letters, Vol. 19, No. 5, 08.05.2019, p. 2858-2870.

Research output: Contribution to journalArticle

Harvard

Rauti, R, Medelin, M, Newman, L, Vranic, S, Reina, G, Bianco, A, Prato, M, Kostarelos, K & Ballerini, L 2019, 'Graphene Oxide Flakes Tune Excitatory Neurotransmission in Vivo by Targeting Hippocampal Synapses', Nano Letters, vol. 19, no. 5, pp. 2858-2870. https://doi.org/10.1021/acs.nanolett.8b04903

APA

Rauti, R., Medelin, M., Newman, L., Vranic, S., Reina, G., Bianco, A., ... Ballerini, L. (2019). Graphene Oxide Flakes Tune Excitatory Neurotransmission in Vivo by Targeting Hippocampal Synapses. Nano Letters, 19(5), 2858-2870. https://doi.org/10.1021/acs.nanolett.8b04903

Vancouver

Author

Rauti, Rossana ; Medelin, Manuela ; Newman, Leon ; Vranic, Sandra ; Reina, Giacomo ; Bianco, Alberto ; Prato, Maurizio ; Kostarelos, Kostas ; Ballerini, Laura. / Graphene Oxide Flakes Tune Excitatory Neurotransmission in Vivo by Targeting Hippocampal Synapses. In: Nano Letters. 2019 ; Vol. 19, No. 5. pp. 2858-2870.

Bibtex

@article{57455a7a33b749d2a5c12f37d1bc9f18,
title = "Graphene Oxide Flakes Tune Excitatory Neurotransmission in Vivo by Targeting Hippocampal Synapses",
abstract = "Synapses compute and transmit information to connect neural circuits and are at the basis of brain operations. Alterations in their function contribute to a vast range of neuropsychiatric and neurodegenerative disorders and synapse-based therapeutic intervention, such as selective inhibition of synaptic transmission, may significantly help against serious pathologies. Graphene is a two-dimensional nanomaterial largely exploited in multiple domains of science and technology, including biomedical applications. In hippocampal neurons in culture, small graphene oxide nanosheets (s-GO) selectively depress glutamatergic activity without altering cell viability. Glutamate is the main excitatory neurotransmitter in the central nervous system and growing evidence suggests its involvement in neuropsychiatric disorders. Here we demonstrate that s-GO directly targets the release of presynaptic vesicle. We propose that s-GO flakes reduce the availability of transmitter, via promoting its fast release and subsequent depletion, leading to a decline ofglutamatergic neurotransmission. We injected s-GO in the hippocampus in vivo, and 48 h after surgery ex vivo patch-clamp recordings from brain slices show a significant reduction in glutamatergic synaptic activity in respect to saline injections.",
keywords = "glutamate, Graphene, hippocampal network, quantum dots, synapses",
author = "Rossana Rauti and Manuela Medelin and Leon Newman and Sandra Vranic and Giacomo Reina and Alberto Bianco and Maurizio Prato and Kostas Kostarelos and Laura Ballerini",
year = "2019",
month = "5",
day = "8",
doi = "10.1021/acs.nanolett.8b04903",
language = "English",
volume = "19",
pages = "2858--2870",
journal = "Nano Letters",
issn = "1530-6984",
publisher = "American Chemical Society",
number = "5",

}

RIS

TY - JOUR

T1 - Graphene Oxide Flakes Tune Excitatory Neurotransmission in Vivo by Targeting Hippocampal Synapses

AU - Rauti, Rossana

AU - Medelin, Manuela

AU - Newman, Leon

AU - Vranic, Sandra

AU - Reina, Giacomo

AU - Bianco, Alberto

AU - Prato, Maurizio

AU - Kostarelos, Kostas

AU - Ballerini, Laura

PY - 2019/5/8

Y1 - 2019/5/8

N2 - Synapses compute and transmit information to connect neural circuits and are at the basis of brain operations. Alterations in their function contribute to a vast range of neuropsychiatric and neurodegenerative disorders and synapse-based therapeutic intervention, such as selective inhibition of synaptic transmission, may significantly help against serious pathologies. Graphene is a two-dimensional nanomaterial largely exploited in multiple domains of science and technology, including biomedical applications. In hippocampal neurons in culture, small graphene oxide nanosheets (s-GO) selectively depress glutamatergic activity without altering cell viability. Glutamate is the main excitatory neurotransmitter in the central nervous system and growing evidence suggests its involvement in neuropsychiatric disorders. Here we demonstrate that s-GO directly targets the release of presynaptic vesicle. We propose that s-GO flakes reduce the availability of transmitter, via promoting its fast release and subsequent depletion, leading to a decline ofglutamatergic neurotransmission. We injected s-GO in the hippocampus in vivo, and 48 h after surgery ex vivo patch-clamp recordings from brain slices show a significant reduction in glutamatergic synaptic activity in respect to saline injections.

AB - Synapses compute and transmit information to connect neural circuits and are at the basis of brain operations. Alterations in their function contribute to a vast range of neuropsychiatric and neurodegenerative disorders and synapse-based therapeutic intervention, such as selective inhibition of synaptic transmission, may significantly help against serious pathologies. Graphene is a two-dimensional nanomaterial largely exploited in multiple domains of science and technology, including biomedical applications. In hippocampal neurons in culture, small graphene oxide nanosheets (s-GO) selectively depress glutamatergic activity without altering cell viability. Glutamate is the main excitatory neurotransmitter in the central nervous system and growing evidence suggests its involvement in neuropsychiatric disorders. Here we demonstrate that s-GO directly targets the release of presynaptic vesicle. We propose that s-GO flakes reduce the availability of transmitter, via promoting its fast release and subsequent depletion, leading to a decline ofglutamatergic neurotransmission. We injected s-GO in the hippocampus in vivo, and 48 h after surgery ex vivo patch-clamp recordings from brain slices show a significant reduction in glutamatergic synaptic activity in respect to saline injections.

KW - glutamate

KW - Graphene

KW - hippocampal network

KW - quantum dots

KW - synapses

U2 - 10.1021/acs.nanolett.8b04903

DO - 10.1021/acs.nanolett.8b04903

M3 - Article

VL - 19

SP - 2858

EP - 2870

JO - Nano Letters

JF - Nano Letters

SN - 1530-6984

IS - 5

ER -