Glycoprotein labeling using engineered variants of galactose oxidase obtained by directed evolution

Research output: Contribution to journalArticle

  • External authors:
  • Julie B. Rannes
  • Avgousta Ioannou
  • Simon C. Willies
  • Gideon Grogan
  • Carsten Behrens

Abstract

A directed evolution approach has been used for the generation of variants of galactose oxidase (GOase) that can selectively oxidize glycans on glycoproteins. The aldehyde function introduced on the glycans d-mannose (Man) and d-N-acetyl glucosamine (GlcNAc) by the enzyme variants could then be used to label the glycoproteins and also whole cells that display mannosides on their surface. © 2011 American Chemical Society.

Bibliographical metadata

Original languageEnglish
Pages (from-to)8436-8439
Number of pages3
JournalJournal of the American Chemical Society
Volume133
Issue number22
DOIs
Publication statusPublished - 8 Jun 2011