Glycolytic flux in Saccharomyces cerevisiae is dependent on RNA polymerase III and its negative regulator Maf1

Research output: Contribution to journalArticle

  • External authors:
  • Roza Szatkowska
  • Manuel Garcia-Albornoz
  • Katarzyna Roszkowska
  • Stephen W. Holman
  • Emil Furmanek
  • Robert J. Beynon
  • Malgorzata Adamczyk

Abstract

Protein biosynthesis is energetically costly, is tightly regulated and is coupled to stress conditions including glucose deprivation. RNA polymerase III (RNAP III)-driven transcription of tDNA genes for production of tRNAs is a key element in efficient protein biosynthesis. Here we present an analysis of the effects of altered RNAP III activity on the Saccharomyces cerevisiae proteome and metabolism under glucose-rich conditions. We show for the first time that RNAP III is tightly coupled to the glycolytic system at the molecular systems level. Decreased RNAP III activity or the absence of the RNAP III negative regulator, Maf1 elicit broad changes in the abundance profiles of enzymes engaged in fundamental metabolism in S. cerevisiae. In a mutant compromised in RNAP III activity, there is a repartitioning towards amino acids synthesis de novo at the expense of glycolytic throughput. Conversely, cells lacking Maf1 protein have greater potential for glycolytic flux.

Bibliographical metadata

Original languageEnglish
Pages (from-to)1053-1082
Number of pages30
JournalBiochemical Journal
Volume476
Issue number7
Early online date4 Apr 2019
DOIs
Publication statusE-pub ahead of print - 4 Apr 2019