Glut-1 and CAIX as intrinsic markers of hypoxia in carcinoma of the cervix: Relationship to pimonidazole bindingCitation formats

  • External authors:
  • Rachel E. Airley
  • Juliette Loncaster
  • James A. Raleigh
  • Adrian L. Harris
  • Susan E. Davidson
  • Robert D. Hunter
  • Catharine M L West

Standard

Glut-1 and CAIX as intrinsic markers of hypoxia in carcinoma of the cervix: Relationship to pimonidazole binding. / West, Catharine; Airley, Rachel E.; Loncaster, Juliette; Raleigh, James A.; Harris, Adrian L.; Davidson, Susan E.; Hunter, Robert D.; West, Catharine M L; Stratford, Ian J.

In: International Journal of Cancer, Vol. 104, No. 1, 10.03.2003, p. 85-91.

Research output: Contribution to journal › Article › peer-review

Harvard

West, C, Airley, RE, Loncaster, J, Raleigh, JA, Harris, AL, Davidson, SE, Hunter, RD, West, CML & Stratford, IJ 2003, 'Glut-1 and CAIX as intrinsic markers of hypoxia in carcinoma of the cervix: Relationship to pimonidazole binding', International Journal of Cancer, vol. 104, no. 1, pp. 85-91. https://doi.org/10.1002/ijc.10904

APA

West, C., Airley, R. E., Loncaster, J., Raleigh, J. A., Harris, A. L., Davidson, S. E., Hunter, R. D., West, C. M. L., & Stratford, I. J. (2003). Glut-1 and CAIX as intrinsic markers of hypoxia in carcinoma of the cervix: Relationship to pimonidazole binding. International Journal of Cancer, 104(1), 85-91. https://doi.org/10.1002/ijc.10904

Vancouver

West C, Airley RE, Loncaster J, Raleigh JA, Harris AL, Davidson SE et al. Glut-1 and CAIX as intrinsic markers of hypoxia in carcinoma of the cervix: Relationship to pimonidazole binding. International Journal of Cancer. 2003 Mar 10;104(1):85-91. https://doi.org/10.1002/ijc.10904

Author

West, Catharine ; Airley, Rachel E. ; Loncaster, Juliette ; Raleigh, James A. ; Harris, Adrian L. ; Davidson, Susan E. ; Hunter, Robert D. ; West, Catharine M L ; Stratford, Ian J. / Glut-1 and CAIX as intrinsic markers of hypoxia in carcinoma of the cervix: Relationship to pimonidazole binding. In: International Journal of Cancer. 2003 ; Vol. 104, No. 1. pp. 85-91.

Bibtex

@article{518b9fe9015c43aeb08b1716f592d1cb,
title = "Glut-1 and CAIX as intrinsic markers of hypoxia in carcinoma of the cervix: Relationship to pimonidazole binding",
abstract = "The presence of hypoxia in tumours results in the overexpression of certain genes, which are controlled via the transcription factor HIF-1. Hypoxic cells are known to be radioresistant and chemoresistant, thus, a reliable surrogate marker of hypoxia is desirable to ensure that treatment may be rationally applied. Recently, the HIF-1-regulated proteins Glut-1 and CAIX were validated as intrinsic markers of hypoxia by comparison with pO2 measured using oxygen electrodes. We compare the expression of Glut-1 and CAIX with the binding of the bioreductive drug hypoxia marker pimonidazole. Pimonidazole was administered to 42 patients with advanced carcinoma of the cervix, 16 hr before biopsy. Sections of single or multiple biopsies were then immunostained for Glut-1 and CAIX, and the area of staining scored by eye, using a {"}field-by-field{"} semi-quantitative averaging system. Using I biopsy only, Glut-1 (r = 0.54, p = <0.001) correlated with the level of pimonidazole binding, and Glut-1 and CAIX expression also correlated significantly (r = 0.40, p = <0.009). Thus, our study has shown that HIF-1 regulated genes have potential for future use as predictors of the malignant changes mediated by hypoxia, and warrant further investigation as indicators of response to cancer therapy. {\textcopyright} 2002 Wiley-Liss, Inc.",
keywords = "CAIX, Glut-1, Hypoxia, Pimonidazole",
author = "Catharine West and Airley, {Rachel E.} and Juliette Loncaster and Raleigh, {James A.} and Harris, {Adrian L.} and Davidson, {Susan E.} and Hunter, {Robert D.} and West, {Catharine M L} and Stratford, {Ian J.}",
year = "2003",
month = mar,
day = "10",
doi = "10.1002/ijc.10904",
language = "English",
volume = "104",
pages = "85--91",
journal = "International journal of cancer. Journal international du cancer",
issn = "0020-7136",
publisher = "John Wiley & Sons Ltd",
number = "1",

}

RIS

TY - JOUR

T1 - Glut-1 and CAIX as intrinsic markers of hypoxia in carcinoma of the cervix: Relationship to pimonidazole binding

AU - West, Catharine

AU - Airley, Rachel E.

AU - Loncaster, Juliette

AU - Raleigh, James A.

AU - Harris, Adrian L.

AU - Davidson, Susan E.

AU - Hunter, Robert D.

AU - West, Catharine M L

AU - Stratford, Ian J.

PY - 2003/3/10

Y1 - 2003/3/10

N2 - The presence of hypoxia in tumours results in the overexpression of certain genes, which are controlled via the transcription factor HIF-1. Hypoxic cells are known to be radioresistant and chemoresistant, thus, a reliable surrogate marker of hypoxia is desirable to ensure that treatment may be rationally applied. Recently, the HIF-1-regulated proteins Glut-1 and CAIX were validated as intrinsic markers of hypoxia by comparison with pO2 measured using oxygen electrodes. We compare the expression of Glut-1 and CAIX with the binding of the bioreductive drug hypoxia marker pimonidazole. Pimonidazole was administered to 42 patients with advanced carcinoma of the cervix, 16 hr before biopsy. Sections of single or multiple biopsies were then immunostained for Glut-1 and CAIX, and the area of staining scored by eye, using a "field-by-field" semi-quantitative averaging system. Using I biopsy only, Glut-1 (r = 0.54, p = <0.001) correlated with the level of pimonidazole binding, and Glut-1 and CAIX expression also correlated significantly (r = 0.40, p = <0.009). Thus, our study has shown that HIF-1 regulated genes have potential for future use as predictors of the malignant changes mediated by hypoxia, and warrant further investigation as indicators of response to cancer therapy. © 2002 Wiley-Liss, Inc.

AB - The presence of hypoxia in tumours results in the overexpression of certain genes, which are controlled via the transcription factor HIF-1. Hypoxic cells are known to be radioresistant and chemoresistant, thus, a reliable surrogate marker of hypoxia is desirable to ensure that treatment may be rationally applied. Recently, the HIF-1-regulated proteins Glut-1 and CAIX were validated as intrinsic markers of hypoxia by comparison with pO2 measured using oxygen electrodes. We compare the expression of Glut-1 and CAIX with the binding of the bioreductive drug hypoxia marker pimonidazole. Pimonidazole was administered to 42 patients with advanced carcinoma of the cervix, 16 hr before biopsy. Sections of single or multiple biopsies were then immunostained for Glut-1 and CAIX, and the area of staining scored by eye, using a "field-by-field" semi-quantitative averaging system. Using I biopsy only, Glut-1 (r = 0.54, p = <0.001) correlated with the level of pimonidazole binding, and Glut-1 and CAIX expression also correlated significantly (r = 0.40, p = <0.009). Thus, our study has shown that HIF-1 regulated genes have potential for future use as predictors of the malignant changes mediated by hypoxia, and warrant further investigation as indicators of response to cancer therapy. © 2002 Wiley-Liss, Inc.

KW - CAIX

KW - Glut-1

KW - Hypoxia

KW - Pimonidazole

U2 - 10.1002/ijc.10904

DO - 10.1002/ijc.10904

M3 - Article

VL - 104

SP - 85

EP - 91

JO - International journal of cancer. Journal international du cancer

JF - International journal of cancer. Journal international du cancer

SN - 0020-7136

IS - 1

ER -