Fibroblast radiosensitivity measured using the comet DNA-damage assay correlates with clonogenic survival parametersCitation formats

  • Authors:
  • A. M. Eastham
  • B. Marples
  • A. E. Kiltie
  • C. J. Orton
  • C. M L West

Standard

Fibroblast radiosensitivity measured using the comet DNA-damage assay correlates with clonogenic survival parameters. / Eastham, A. M.; Marples, B.; Kiltie, A. E.; Orton, C. J.; West, C. M L.

In: British Journal of Cancer, Vol. 79, No. 9-10, 1999, p. 1366-1371.

Research output: Contribution to journalArticlepeer-review

Harvard

Eastham, AM, Marples, B, Kiltie, AE, Orton, CJ & West, CML 1999, 'Fibroblast radiosensitivity measured using the comet DNA-damage assay correlates with clonogenic survival parameters', British Journal of Cancer, vol. 79, no. 9-10, pp. 1366-1371.

APA

Eastham, A. M., Marples, B., Kiltie, A. E., Orton, C. J., & West, C. M. L. (1999). Fibroblast radiosensitivity measured using the comet DNA-damage assay correlates with clonogenic survival parameters. British Journal of Cancer, 79(9-10), 1366-1371.

Vancouver

Eastham AM, Marples B, Kiltie AE, Orton CJ, West CML. Fibroblast radiosensitivity measured using the comet DNA-damage assay correlates with clonogenic survival parameters. British Journal of Cancer. 1999;79(9-10):1366-1371.

Author

Eastham, A. M. ; Marples, B. ; Kiltie, A. E. ; Orton, C. J. ; West, C. M L. / Fibroblast radiosensitivity measured using the comet DNA-damage assay correlates with clonogenic survival parameters. In: British Journal of Cancer. 1999 ; Vol. 79, No. 9-10. pp. 1366-1371.

Bibtex

@article{f7c07d11431a4b4a889d00fb991dd18a,
title = "Fibroblast radiosensitivity measured using the comet DNA-damage assay correlates with clonogenic survival parameters",
abstract = "A study was made of the neutral comet assay as a potential method for measuring normal cell radiosensitivity. Eleven fibroblast strains were studied comprising nine derived from vaginal biopsies from pretreatment cervical cancer patients and two strains from radiosensitive individuals. DNA double strand break (dsbs) dose-response curves for both initial and residual (20-h repair time) damage were obtained over the dose range 0-240 Gy, with slopes varying 3.2 and 8-fold respectively. Clonogenic cell survival parameters were available for all the cell strains following both high- and low-dose rate irradiation. There were no correlations between the dose-response slope of the initial level of DNA dsbs and parameters that mainly describe the initial portion of clonogenic radiation survival curves (SF2, α, D). A significant correlation (r = -0.63, P = 0.04) was found between the extent of residual DNA dsbs and clonogenicity for all 11 fibroblast strains. The parameter showing the highest correlation with fibroblast cell killing (D) for the nine normal fibroblasts alone was the ratio of initial/residual DNA dsb dose-response slope (r = 0.80, P = <0.01). A significant correlation (r = -0.67, P = 0.03) with clonogenic radiosensitivity was also found for all 11 cell strains when using the ratio of initial/residual DNA dsb damage at a single dose of 180 Gy. This study shows that fibroblast radiosensitivity measured using the neutral comet assay correlates with clonogenic radiation survival parameters, and therefore may have potential value in predictive testing of normal tissue radiosensitivity.",
keywords = "Comet assay, DNA damage, Fibroblasts, Intrinsic radiosensitivity, Predictive assays",
author = "Eastham, {A. M.} and B. Marples and Kiltie, {A. E.} and Orton, {C. J.} and West, {C. M L}",
year = "1999",
language = "English",
volume = "79",
pages = "1366--1371",
journal = "BJC",
issn = "0007-0920",
publisher = "Springer Nature",
number = "9-10",

}

RIS

TY - JOUR

T1 - Fibroblast radiosensitivity measured using the comet DNA-damage assay correlates with clonogenic survival parameters

AU - Eastham, A. M.

AU - Marples, B.

AU - Kiltie, A. E.

AU - Orton, C. J.

AU - West, C. M L

PY - 1999

Y1 - 1999

N2 - A study was made of the neutral comet assay as a potential method for measuring normal cell radiosensitivity. Eleven fibroblast strains were studied comprising nine derived from vaginal biopsies from pretreatment cervical cancer patients and two strains from radiosensitive individuals. DNA double strand break (dsbs) dose-response curves for both initial and residual (20-h repair time) damage were obtained over the dose range 0-240 Gy, with slopes varying 3.2 and 8-fold respectively. Clonogenic cell survival parameters were available for all the cell strains following both high- and low-dose rate irradiation. There were no correlations between the dose-response slope of the initial level of DNA dsbs and parameters that mainly describe the initial portion of clonogenic radiation survival curves (SF2, α, D). A significant correlation (r = -0.63, P = 0.04) was found between the extent of residual DNA dsbs and clonogenicity for all 11 fibroblast strains. The parameter showing the highest correlation with fibroblast cell killing (D) for the nine normal fibroblasts alone was the ratio of initial/residual DNA dsb dose-response slope (r = 0.80, P = <0.01). A significant correlation (r = -0.67, P = 0.03) with clonogenic radiosensitivity was also found for all 11 cell strains when using the ratio of initial/residual DNA dsb damage at a single dose of 180 Gy. This study shows that fibroblast radiosensitivity measured using the neutral comet assay correlates with clonogenic radiation survival parameters, and therefore may have potential value in predictive testing of normal tissue radiosensitivity.

AB - A study was made of the neutral comet assay as a potential method for measuring normal cell radiosensitivity. Eleven fibroblast strains were studied comprising nine derived from vaginal biopsies from pretreatment cervical cancer patients and two strains from radiosensitive individuals. DNA double strand break (dsbs) dose-response curves for both initial and residual (20-h repair time) damage were obtained over the dose range 0-240 Gy, with slopes varying 3.2 and 8-fold respectively. Clonogenic cell survival parameters were available for all the cell strains following both high- and low-dose rate irradiation. There were no correlations between the dose-response slope of the initial level of DNA dsbs and parameters that mainly describe the initial portion of clonogenic radiation survival curves (SF2, α, D). A significant correlation (r = -0.63, P = 0.04) was found between the extent of residual DNA dsbs and clonogenicity for all 11 fibroblast strains. The parameter showing the highest correlation with fibroblast cell killing (D) for the nine normal fibroblasts alone was the ratio of initial/residual DNA dsb dose-response slope (r = 0.80, P = <0.01). A significant correlation (r = -0.67, P = 0.03) with clonogenic radiosensitivity was also found for all 11 cell strains when using the ratio of initial/residual DNA dsb damage at a single dose of 180 Gy. This study shows that fibroblast radiosensitivity measured using the neutral comet assay correlates with clonogenic radiation survival parameters, and therefore may have potential value in predictive testing of normal tissue radiosensitivity.

KW - Comet assay

KW - DNA damage

KW - Fibroblasts

KW - Intrinsic radiosensitivity

KW - Predictive assays

M3 - Article

VL - 79

SP - 1366

EP - 1371

JO - BJC

JF - BJC

SN - 0007-0920

IS - 9-10

ER -