Extracellular vesicles induce protective immunity against Trichuris murisCitation formats

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Extracellular vesicles induce protective immunity against Trichuris muris. / Shears, Rebecca; Bancroft, Allison; Hughes, G. ; Grencis, Richard; Thornton, David.

In: Parasite Immunology, Vol. 40, No. 7, 27.06.2018.

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@article{647f22b26a214a2fa44b51a1468ad2f9,
title = "Extracellular vesicles induce protective immunity against Trichuris muris",
abstract = "Gastrointestinal nematodes, such as Trichuris trichiura (human whipworm), are a major source of morbidity in humans and their livestock. There is a paucity of commercially available vaccines against these parasites, and vaccine development for T. trichiura has been impeded by a lack of known host protective antigens. Experimental vaccinations with T. muris (murine whipworm) soluble Excretory/Secretory (ES) material have demonstrated that it is possible to induce protective immunity in mice; however, the potential for extracellular vesicles (EVs) as a source of antigenic material has remained relatively unexplored. Here, we demonstrate that EVs isolated from T. muris ES can induce protective immunity in mice when administered as a vaccine without adjuvant and show that the protective properties of these EVs are dependent on intact vesicles. We also identified several proteins within EV preparations that are targeted by the host antibodies following vaccination and subsequent infection with T. muris. Many of these proteins, including VWD and vitellogenin N and DUF1943‐domain‐containing protein, vacuolar protein sorting‐associated protein 52 and TSP‐1 domain‐containing protein, were detected in both soluble ES and EV samples and have homologues in other parasites of medical and veterinary importance, and as such are possible protective antigens.",
author = "Rebecca Shears and Allison Bancroft and G. Hughes and Richard Grencis and David Thornton",
year = "2018",
month = jun,
day = "27",
doi = "10.1111/pim.12536",
language = "English",
volume = "40",
journal = "Parasite Immunology",
issn = "1365-3024",
publisher = "John Wiley & Sons Ltd",
number = "7",

}

RIS

TY - JOUR

T1 - Extracellular vesicles induce protective immunity against Trichuris muris

AU - Shears, Rebecca

AU - Bancroft, Allison

AU - Hughes, G.

AU - Grencis, Richard

AU - Thornton, David

PY - 2018/6/27

Y1 - 2018/6/27

N2 - Gastrointestinal nematodes, such as Trichuris trichiura (human whipworm), are a major source of morbidity in humans and their livestock. There is a paucity of commercially available vaccines against these parasites, and vaccine development for T. trichiura has been impeded by a lack of known host protective antigens. Experimental vaccinations with T. muris (murine whipworm) soluble Excretory/Secretory (ES) material have demonstrated that it is possible to induce protective immunity in mice; however, the potential for extracellular vesicles (EVs) as a source of antigenic material has remained relatively unexplored. Here, we demonstrate that EVs isolated from T. muris ES can induce protective immunity in mice when administered as a vaccine without adjuvant and show that the protective properties of these EVs are dependent on intact vesicles. We also identified several proteins within EV preparations that are targeted by the host antibodies following vaccination and subsequent infection with T. muris. Many of these proteins, including VWD and vitellogenin N and DUF1943‐domain‐containing protein, vacuolar protein sorting‐associated protein 52 and TSP‐1 domain‐containing protein, were detected in both soluble ES and EV samples and have homologues in other parasites of medical and veterinary importance, and as such are possible protective antigens.

AB - Gastrointestinal nematodes, such as Trichuris trichiura (human whipworm), are a major source of morbidity in humans and their livestock. There is a paucity of commercially available vaccines against these parasites, and vaccine development for T. trichiura has been impeded by a lack of known host protective antigens. Experimental vaccinations with T. muris (murine whipworm) soluble Excretory/Secretory (ES) material have demonstrated that it is possible to induce protective immunity in mice; however, the potential for extracellular vesicles (EVs) as a source of antigenic material has remained relatively unexplored. Here, we demonstrate that EVs isolated from T. muris ES can induce protective immunity in mice when administered as a vaccine without adjuvant and show that the protective properties of these EVs are dependent on intact vesicles. We also identified several proteins within EV preparations that are targeted by the host antibodies following vaccination and subsequent infection with T. muris. Many of these proteins, including VWD and vitellogenin N and DUF1943‐domain‐containing protein, vacuolar protein sorting‐associated protein 52 and TSP‐1 domain‐containing protein, were detected in both soluble ES and EV samples and have homologues in other parasites of medical and veterinary importance, and as such are possible protective antigens.

U2 - 10.1111/pim.12536

DO - 10.1111/pim.12536

M3 - Article

VL - 40

JO - Parasite Immunology

JF - Parasite Immunology

SN - 1365-3024

IS - 7

ER -