Estimating fungal growth in submerged fermentation in the presence of solid particles based on colour developmentCitation formats

TY - JOUR

T1 - Estimating fungal growth in submerged fermentation in the presence of solid particles based on colour development

AU - Abdul Manan, Musaalbakri

AU - Webb, Colin

PY - 2018

Y1 - 2018

N2 - This study examines and evaluates a colourimetric technique that could be implemented as a real-time continuous monitoring system for fungal culture growth in submerged fermentation (SmF) with the presence of solid particles. Fungal SmF of Aspergillus awamori and Aspergillus oryzae was carried out on complex heterogeneous solid media: wheat bran, soybean hull and rapeseed meal, which were constituted of various soluble and insoluble solid particles. As a comparison, another set of experiments in SmF using synthetic medium was carried out. The theory of measuring colour changes in SmF by UV-Vis spectroscopy demonstrates that this colourimetric technique provides valuable information complementary to visual observations. Typical normalized UV absorption spectra were obtained using a scanning spectrophotometer for each colour solution. These demonstrated that samples used in this work have very similar patterns. The typical normalized optical density spectra show a local absorbance maximum near 300 nm, a region where colour absorbance rapidly increases. It was suggested that the colour developed during the progress of fermentation is closely related to either or both of the growth and the actual biomass of the fungus. The findings show that colour-based methods seem to be the most promising approach for biomass estimation in SmF in the presence of solid particles.

AB - This study examines and evaluates a colourimetric technique that could be implemented as a real-time continuous monitoring system for fungal culture growth in submerged fermentation (SmF) with the presence of solid particles. Fungal SmF of Aspergillus awamori and Aspergillus oryzae was carried out on complex heterogeneous solid media: wheat bran, soybean hull and rapeseed meal, which were constituted of various soluble and insoluble solid particles. As a comparison, another set of experiments in SmF using synthetic medium was carried out. The theory of measuring colour changes in SmF by UV-Vis spectroscopy demonstrates that this colourimetric technique provides valuable information complementary to visual observations. Typical normalized UV absorption spectra were obtained using a scanning spectrophotometer for each colour solution. These demonstrated that samples used in this work have very similar patterns. The typical normalized optical density spectra show a local absorbance maximum near 300 nm, a region where colour absorbance rapidly increases. It was suggested that the colour developed during the progress of fermentation is closely related to either or both of the growth and the actual biomass of the fungus. The findings show that colour-based methods seem to be the most promising approach for biomass estimation in SmF in the presence of solid particles.

KW - Aspergillus awamori

KW - Aspergillus oryzae

KW - colourimetric technique

KW - Growth estimating

KW - solid particles

KW - submerged fermentation

UR - http://www.scopus.com/inward/record.url?scp=85042459106&partnerID=8YFLogxK

U2 - 10.1080/13102818.2018.1440974

DO - 10.1080/13102818.2018.1440974

M3 - Article

AN - SCOPUS:85042459106

VL - 32

SP - 618

EP - 627

JO - Biotechnology and Biotechnological Equipment

JF - Biotechnology and Biotechnological Equipment

SN - 1310-2818

IS - 3

ER -