Enzymatic synthesis of N-acetyllactosamine from lactose enabled by recombinant β1,4-galactosyltransferases

Research output: Contribution to journalArticle

  • External authors:
  • Kun Huang
  • Helene Ledru
  • Kristian Hollingsworth
  • Jordi Mas Pons
  • Andrea Marchesi
  • Peter Both
  • Ashley P Mattey
  • Edward Pallister
  • Gregory S Bulmer
  • W Bruce Turnbull
  • M. Carmen Galan

Abstract

Utilising a fast and sensitive screening method based on imidazolium-tagged probes, we report unprecedented reversible activity of bacterial β1,4-galactosyltransferases to catalyse the transgalactosylation from lactose to N-acetylglucosamine to form N-acetyllactosamine in the presence of UDP. The process is demonstrated by the preparative scale synthesis of pNP-β-LacNAcm from lactose using β1,4-galactosyltransferase NmLgtB-B as the only biocatalyst.

Bibliographical metadata

Original languageEnglish
JournalOrganic & biomolecular chemistry
Early online date30 May 2019
DOIs
Publication statusPublished - 2019

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