Engineering and improvement of the efficiency of a chimeric [P450cam-RhFRed reductase domain] enzyme

Research output: Contribution to journalArticle

  • External authors:
  • Aélig Robin
  • Gareth A. Roberts
  • Johannes Kisch
  • Federico Sabbadin
  • Gideon Grogan
  • Neil Bruce

Abstract

A chimeric oxygenase, in which the P450cam domain was fused to the reductase host domains of a P450RhF from Rhodococcus sp. strain NCIMB 9784 was optimised to allow for a biotransformation at 30 mM substrate in 80% overall yield, with the linker region between P450 and FMN domain proving to be important for the effective biotransformation of (+)-camphor to 5-exo-hydroxycamphor. © The Royal Society of Chemistry 2009.

Bibliographical metadata

Original languageEnglish
Pages (from-to)2478-2480
Number of pages2
JournalChemical Communications
Issue number18
DOIs
Publication statusPublished - 2009