Ena orchestrates remodelling within the actin cytoskeleton to drive robust Drosophila macrophage chemotaxisCitation formats

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Ena orchestrates remodelling within the actin cytoskeleton to drive robust Drosophila macrophage chemotaxis. / Davidson, Andrew; Millard, Thomas; Evans, Iwan; Wood, William.

In: Journal of Cell Science, Vol. 132, No. 5, 18.02.2019.

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Davidson, Andrew ; Millard, Thomas ; Evans, Iwan ; Wood, William. / Ena orchestrates remodelling within the actin cytoskeleton to drive robust Drosophila macrophage chemotaxis. In: Journal of Cell Science. 2019 ; Vol. 132, No. 5.

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@article{d51f849dc8c044a4afdb3cb9f540adc4,
title = "Ena orchestrates remodelling within the actin cytoskeleton to drive robust Drosophila macrophage chemotaxis",
abstract = "The actin cytoskeleton is the engine that powers the inflammatory chemotaxis of immune cells to sites of tissue damage or infection. Here, we combine genetics with live in vivo imaging to investigate how cytoskeletal rearrangements drive macrophage recruitment to wounds in Drosophila. We find that the actin-regulatory protein Ena is a master regulator of lamellipodial dynamics in migrating macrophages, where it remodels the cytoskeleton to form linear filaments that can then be bundled together by the cross-linker Fascin (also known as Singed in flies). In contrast, the formin Dia generates rare, probing filopods for specialised functions that are not required for migration. The role of Ena in lamellipodial bundling is so fundamental that its overexpression increases bundling even in the absence of Fascin by marshalling the remaining cross-linking proteins to compensate. This reorganisation of the lamellipod generates cytoskeletal struts that push against the membrane to drive leading edge advancement and boost cell speed. Thus, Ena-mediated remodelling extracts the most from the cytoskeleton to power robust macrophage chemotaxis during their inflammatory recruitment to wounds.",
keywords = "Actin, Drosophila, Ena, Hemocyte, Macrophage, Migration",
author = "Andrew Davidson and Thomas Millard and Iwan Evans and William Wood",
year = "2019",
month = feb,
day = "18",
doi = "10.1242/jcs.224618",
language = "English",
volume = "132",
journal = "Journal of Cell Science",
issn = "0021-9533",
publisher = "Company of Biologists Ltd",
number = "5",

}

RIS

TY - JOUR

T1 - Ena orchestrates remodelling within the actin cytoskeleton to drive robust Drosophila macrophage chemotaxis

AU - Davidson, Andrew

AU - Millard, Thomas

AU - Evans, Iwan

AU - Wood, William

PY - 2019/2/18

Y1 - 2019/2/18

N2 - The actin cytoskeleton is the engine that powers the inflammatory chemotaxis of immune cells to sites of tissue damage or infection. Here, we combine genetics with live in vivo imaging to investigate how cytoskeletal rearrangements drive macrophage recruitment to wounds in Drosophila. We find that the actin-regulatory protein Ena is a master regulator of lamellipodial dynamics in migrating macrophages, where it remodels the cytoskeleton to form linear filaments that can then be bundled together by the cross-linker Fascin (also known as Singed in flies). In contrast, the formin Dia generates rare, probing filopods for specialised functions that are not required for migration. The role of Ena in lamellipodial bundling is so fundamental that its overexpression increases bundling even in the absence of Fascin by marshalling the remaining cross-linking proteins to compensate. This reorganisation of the lamellipod generates cytoskeletal struts that push against the membrane to drive leading edge advancement and boost cell speed. Thus, Ena-mediated remodelling extracts the most from the cytoskeleton to power robust macrophage chemotaxis during their inflammatory recruitment to wounds.

AB - The actin cytoskeleton is the engine that powers the inflammatory chemotaxis of immune cells to sites of tissue damage or infection. Here, we combine genetics with live in vivo imaging to investigate how cytoskeletal rearrangements drive macrophage recruitment to wounds in Drosophila. We find that the actin-regulatory protein Ena is a master regulator of lamellipodial dynamics in migrating macrophages, where it remodels the cytoskeleton to form linear filaments that can then be bundled together by the cross-linker Fascin (also known as Singed in flies). In contrast, the formin Dia generates rare, probing filopods for specialised functions that are not required for migration. The role of Ena in lamellipodial bundling is so fundamental that its overexpression increases bundling even in the absence of Fascin by marshalling the remaining cross-linking proteins to compensate. This reorganisation of the lamellipod generates cytoskeletal struts that push against the membrane to drive leading edge advancement and boost cell speed. Thus, Ena-mediated remodelling extracts the most from the cytoskeleton to power robust macrophage chemotaxis during their inflammatory recruitment to wounds.

KW - Actin

KW - Drosophila

KW - Ena

KW - Hemocyte

KW - Macrophage

KW - Migration

U2 - 10.1242/jcs.224618

DO - 10.1242/jcs.224618

M3 - Article

VL - 132

JO - Journal of Cell Science

JF - Journal of Cell Science

SN - 0021-9533

IS - 5

ER -