EMMA assembly explained: A step-by-step guide to assemble synthetic mammalian vectors

Research output: Contribution to journalArticle

Abstract

Construction of expression vectors is imperative for many areas of biological research and the biotechnology industry. Modular cloning systems for expression vector construction offer a labor- and cost-effective alternative to overcome drawbacks associated with traditional cloning methods. We developed an Extensible Mammalian Modular Assembly toolkit (EMMA) as an efficient and versatile tool to facilitate the construction of functionally diverse mammalian expression vectors from a standardized library of DNA parts. This system supports both hierarchical and combinatorial assembly, and is adaptable for automation. In this chapter, we describe the protocols to construct libraries of DNA parts and assemble these parts into mammalian expression vectors using EMMA.

Bibliographical metadata

Original languageEnglish
Pages (from-to)463-493
Number of pages31
JournalMethods in Enzymology
Volume617
Early online date13 Feb 2019
DOIs
Publication statusPublished - 2019