Effects of radiographic contrast media on proliferation and apoptosis of human vascular endothelial cellsCitation formats

  • Authors:
  • H. Zhang
  • C. M. Holt
  • N. Malik
  • L. Shepherd
  • S. K. Morcos

Standard

Effects of radiographic contrast media on proliferation and apoptosis of human vascular endothelial cells. / Zhang, H.; Holt, C. M.; Malik, N.; Shepherd, L.; Morcos, S. K.

In: British Journal of Radiology, Vol. 73, No. 874, 2000, p. 1034-1041.

Research output: Contribution to journal › Article › peer-review

Harvard

Zhang, H, Holt, CM, Malik, N, Shepherd, L & Morcos, SK 2000, 'Effects of radiographic contrast media on proliferation and apoptosis of human vascular endothelial cells', British Journal of Radiology, vol. 73, no. 874, pp. 1034-1041.

APA

Zhang, H., Holt, C. M., Malik, N., Shepherd, L., & Morcos, S. K. (2000). Effects of radiographic contrast media on proliferation and apoptosis of human vascular endothelial cells. British Journal of Radiology, 73(874), 1034-1041.

Vancouver

Zhang H, Holt CM, Malik N, Shepherd L, Morcos SK. Effects of radiographic contrast media on proliferation and apoptosis of human vascular endothelial cells. British Journal of Radiology. 2000;73(874):1034-1041.

Author

Zhang, H. ; Holt, C. M. ; Malik, N. ; Shepherd, L. ; Morcos, S. K. / Effects of radiographic contrast media on proliferation and apoptosis of human vascular endothelial cells. In: British Journal of Radiology. 2000 ; Vol. 73, No. 874. pp. 1034-1041.

Bibtex

@article{12f3a3d3c22648cf80db4d393f986593,
title = "Effects of radiographic contrast media on proliferation and apoptosis of human vascular endothelial cells",
abstract = "The aim of the study was to determine the effects of radiographic contrast media (RCM) on proliferation and apoptosis of human vascular endothelial cells. Human umbilical vein endothelial cells (HUVECs) were exposed for either 1 min or 15 min to RCM (diatrizoate, ioxaglate, iopromide, iotrolan) at an iodine concentration of 250 mgI ml-1. Controls were complete growth medium (CGM) and saturated mannitol (osmotic control). [3H]thymidine incorporation was used to determine cell proliferation 24 h after exposure. Apoptosis was determined at 1 h and 6 h by terminal uridine nick end labelling (TUNEL), time lapse video microscopy (TLVM) and DNA electrophoresis. Mean proliferation rates (%) (±SEM) (p-values compared with the CGM control) at 1 min and 15 min, respectively, were: diatrizoate: 31.9 (10.6), 5.8 (1.5) (pioxaglate>iopromide) reduced proliferation and increased apoptosis of HUVECs. The effects were more pronounced with ionic RCM and seem to depend on osmolality as well as the chemical structure of these agents. Endothelial injury and apoptosis may be responsible for some of the side effects associated with intravascular use of RCM.",
author = "H. Zhang and Holt, {C. M.} and N. Malik and L. Shepherd and Morcos, {S. K.}",
year = "2000",
language = "English",
volume = "73",
pages = "1034--1041",
journal = "British Journal of Radiology",
issn = "0007-1285",
publisher = "British Institute of Radiology",
number = "874",

}

RIS

TY - JOUR

T1 - Effects of radiographic contrast media on proliferation and apoptosis of human vascular endothelial cells

AU - Zhang, H.

AU - Holt, C. M.

AU - Malik, N.

AU - Shepherd, L.

AU - Morcos, S. K.

PY - 2000

Y1 - 2000

N2 - The aim of the study was to determine the effects of radiographic contrast media (RCM) on proliferation and apoptosis of human vascular endothelial cells. Human umbilical vein endothelial cells (HUVECs) were exposed for either 1 min or 15 min to RCM (diatrizoate, ioxaglate, iopromide, iotrolan) at an iodine concentration of 250 mgI ml-1. Controls were complete growth medium (CGM) and saturated mannitol (osmotic control). [3H]thymidine incorporation was used to determine cell proliferation 24 h after exposure. Apoptosis was determined at 1 h and 6 h by terminal uridine nick end labelling (TUNEL), time lapse video microscopy (TLVM) and DNA electrophoresis. Mean proliferation rates (%) (±SEM) (p-values compared with the CGM control) at 1 min and 15 min, respectively, were: diatrizoate: 31.9 (10.6), 5.8 (1.5) (pioxaglate>iopromide) reduced proliferation and increased apoptosis of HUVECs. The effects were more pronounced with ionic RCM and seem to depend on osmolality as well as the chemical structure of these agents. Endothelial injury and apoptosis may be responsible for some of the side effects associated with intravascular use of RCM.

AB - The aim of the study was to determine the effects of radiographic contrast media (RCM) on proliferation and apoptosis of human vascular endothelial cells. Human umbilical vein endothelial cells (HUVECs) were exposed for either 1 min or 15 min to RCM (diatrizoate, ioxaglate, iopromide, iotrolan) at an iodine concentration of 250 mgI ml-1. Controls were complete growth medium (CGM) and saturated mannitol (osmotic control). [3H]thymidine incorporation was used to determine cell proliferation 24 h after exposure. Apoptosis was determined at 1 h and 6 h by terminal uridine nick end labelling (TUNEL), time lapse video microscopy (TLVM) and DNA electrophoresis. Mean proliferation rates (%) (±SEM) (p-values compared with the CGM control) at 1 min and 15 min, respectively, were: diatrizoate: 31.9 (10.6), 5.8 (1.5) (pioxaglate>iopromide) reduced proliferation and increased apoptosis of HUVECs. The effects were more pronounced with ionic RCM and seem to depend on osmolality as well as the chemical structure of these agents. Endothelial injury and apoptosis may be responsible for some of the side effects associated with intravascular use of RCM.

M3 - Article

C2 - 11271894

VL - 73

SP - 1034

EP - 1041

JO - British Journal of Radiology

JF - British Journal of Radiology

SN - 0007-1285

IS - 874

ER -