Dynamics of Intact Immunoglobulin G Explored by Drift-Tube Ion-Mobility Mass Spectrometry and Molecular Modeling

Research output: Research - peer-reviewArticle

  • External authors:
  • K J Pacholarz
  • M Porrini
  • R A Garlish
  • R J Burnley
  • R J Taylor
  • A J Henry


Collision cross-sections (CCS) of immunoglobulins G1 and G4 have been determined using linear drift-tube ion-mobility mass spectrometry. Intact antibodies and Fc-hinge fragments present with a larger range of CCS than proteins of comparable size. This is rationalized with MD simulations, which indicate significant in vacuo dynamics between linked folded domains. The IgG4 subclass presents over a wider CCS range than the IgG1 subclass.

Bibliographical metadata

Original languageEnglish
Pages (from-to)7765-7769
Number of pages5
JournalAngewandte Chemie-International Edition
Issue number30
StatePublished - 2014