Dust mite allergens are carried on not only large particlesCitation formats

  • External authors:
  • Adnan Custovic
  • Hannah Woodcock
  • Mark Craven
  • Rebecca Hassall
  • Elizabeth Hadley
  • Ashley Woodcock

Standard

Dust mite allergens are carried on not only large particles. / Custovic, Adnan; Woodcock, Hannah; Craven, Mark; Hassall, Rebecca; Hadley, Elizabeth; Simpson, Angela; Woodcock, Ashley.

In: Pediatric Allergy and Immunology, Vol. 10, No. 4, 11.1999, p. 258-260.

Research output: Contribution to journalArticle

Harvard

Custovic, A, Woodcock, H, Craven, M, Hassall, R, Hadley, E, Simpson, A & Woodcock, A 1999, 'Dust mite allergens are carried on not only large particles' Pediatric Allergy and Immunology, vol. 10, no. 4, pp. 258-260. https://doi.org/10.1034/j.1399-3038.1999.00050.x

APA

Custovic, A., Woodcock, H., Craven, M., Hassall, R., Hadley, E., Simpson, A., & Woodcock, A. (1999). Dust mite allergens are carried on not only large particles. Pediatric Allergy and Immunology, 10(4), 258-260. https://doi.org/10.1034/j.1399-3038.1999.00050.x

Vancouver

Custovic A, Woodcock H, Craven M, Hassall R, Hadley E, Simpson A et al. Dust mite allergens are carried on not only large particles. Pediatric Allergy and Immunology. 1999 Nov;10(4):258-260. https://doi.org/10.1034/j.1399-3038.1999.00050.x

Author

Custovic, Adnan ; Woodcock, Hannah ; Craven, Mark ; Hassall, Rebecca ; Hadley, Elizabeth ; Simpson, Angela ; Woodcock, Ashley. / Dust mite allergens are carried on not only large particles. In: Pediatric Allergy and Immunology. 1999 ; Vol. 10, No. 4. pp. 258-260.

Bibtex

@article{9bcf98902f344468803ad1b5f55e34f5,
title = "Dust mite allergens are carried on not only large particles",
abstract = "The major obstacle for the successful measurement of airborne mite allergen is its very low concentration in the absence of vigorous disturbance. The aim of this study was to investigate the particle size distribution of group 2 dust mite allergen using an amplified ELISA system. Air sampling was performed using an Andersen sampler placed in the centre of the room, 1.2 m above floor level (airflow rate 28.7 l/min). This is a multistage, multiorifice cascade impactor that is comprised of six stages. Any particle greater that 4.7 μm should impact on stages 1 and 2, whilst stages 3-6 measure the predominantly respiratory range. The sampling was carried out for 30 min after 15 min of vigorous disturbance (vacuum cleaning without bag and filter). Der p 2 was measured using mAb-based ELISA with the AmpliQ amplification kit (Dako Ltd, Cambridgeshire, UK). The sensitivity was increased 15-fold as compared with standard assay, bringing the level of detection to 300 pg/ml. The majority of airborne Der p 2 (79.4{\%}) was carried on large particles (> 4.7 μm). However, a small but important proportion of airborne Der p 2 (20.6{\%}) was associated with small particles (1.1-4.7 μm). It is worth noting that all the levels measured were below the detection limit of standard assay. In conclusion, we have shown that using an amplification system, airborne mite allergen previously undetectable owing to its low concentration can be quantified. Group 2 dust mite allergen is carried not only on large particles. A small, but potentially significant proportion of this airborne allergen is associated with small particles which, when inhaled, may penetrate deep into the human respiratory tract.",
keywords = "Aerodynamics, Allergens, Der p 2, Mite",
author = "Adnan Custovic and Hannah Woodcock and Mark Craven and Rebecca Hassall and Elizabeth Hadley and Angela Simpson and Ashley Woodcock",
year = "1999",
month = "11",
doi = "10.1034/j.1399-3038.1999.00050.x",
language = "English",
volume = "10",
pages = "258--260",
journal = "Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology",
issn = "0905-6157",
publisher = "Blackwell Munksgaard",
number = "4",

}

RIS

TY - JOUR

T1 - Dust mite allergens are carried on not only large particles

AU - Custovic, Adnan

AU - Woodcock, Hannah

AU - Craven, Mark

AU - Hassall, Rebecca

AU - Hadley, Elizabeth

AU - Simpson, Angela

AU - Woodcock, Ashley

PY - 1999/11

Y1 - 1999/11

N2 - The major obstacle for the successful measurement of airborne mite allergen is its very low concentration in the absence of vigorous disturbance. The aim of this study was to investigate the particle size distribution of group 2 dust mite allergen using an amplified ELISA system. Air sampling was performed using an Andersen sampler placed in the centre of the room, 1.2 m above floor level (airflow rate 28.7 l/min). This is a multistage, multiorifice cascade impactor that is comprised of six stages. Any particle greater that 4.7 μm should impact on stages 1 and 2, whilst stages 3-6 measure the predominantly respiratory range. The sampling was carried out for 30 min after 15 min of vigorous disturbance (vacuum cleaning without bag and filter). Der p 2 was measured using mAb-based ELISA with the AmpliQ amplification kit (Dako Ltd, Cambridgeshire, UK). The sensitivity was increased 15-fold as compared with standard assay, bringing the level of detection to 300 pg/ml. The majority of airborne Der p 2 (79.4%) was carried on large particles (> 4.7 μm). However, a small but important proportion of airborne Der p 2 (20.6%) was associated with small particles (1.1-4.7 μm). It is worth noting that all the levels measured were below the detection limit of standard assay. In conclusion, we have shown that using an amplification system, airborne mite allergen previously undetectable owing to its low concentration can be quantified. Group 2 dust mite allergen is carried not only on large particles. A small, but potentially significant proportion of this airborne allergen is associated with small particles which, when inhaled, may penetrate deep into the human respiratory tract.

AB - The major obstacle for the successful measurement of airborne mite allergen is its very low concentration in the absence of vigorous disturbance. The aim of this study was to investigate the particle size distribution of group 2 dust mite allergen using an amplified ELISA system. Air sampling was performed using an Andersen sampler placed in the centre of the room, 1.2 m above floor level (airflow rate 28.7 l/min). This is a multistage, multiorifice cascade impactor that is comprised of six stages. Any particle greater that 4.7 μm should impact on stages 1 and 2, whilst stages 3-6 measure the predominantly respiratory range. The sampling was carried out for 30 min after 15 min of vigorous disturbance (vacuum cleaning without bag and filter). Der p 2 was measured using mAb-based ELISA with the AmpliQ amplification kit (Dako Ltd, Cambridgeshire, UK). The sensitivity was increased 15-fold as compared with standard assay, bringing the level of detection to 300 pg/ml. The majority of airborne Der p 2 (79.4%) was carried on large particles (> 4.7 μm). However, a small but important proportion of airborne Der p 2 (20.6%) was associated with small particles (1.1-4.7 μm). It is worth noting that all the levels measured were below the detection limit of standard assay. In conclusion, we have shown that using an amplification system, airborne mite allergen previously undetectable owing to its low concentration can be quantified. Group 2 dust mite allergen is carried not only on large particles. A small, but potentially significant proportion of this airborne allergen is associated with small particles which, when inhaled, may penetrate deep into the human respiratory tract.

KW - Aerodynamics

KW - Allergens

KW - Der p 2

KW - Mite

U2 - 10.1034/j.1399-3038.1999.00050.x

DO - 10.1034/j.1399-3038.1999.00050.x

M3 - Article

VL - 10

SP - 258

EP - 260

JO - Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology

JF - Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology

SN - 0905-6157

IS - 4

ER -