Drosophila ovarian germline stem cell cytocensor projections dynamically receive and attenuate BMP signallingCitation formats

Standard

Drosophila ovarian germline stem cell cytocensor projections dynamically receive and attenuate BMP signalling. / Wilcockson, Scott; Ashe, Hilary.

In: Developmental cell, 06.06.2019.

Research output: Contribution to journalArticle

Harvard

APA

Vancouver

Author

Bibtex

@article{d9160ea916024053ae467142e7879da0,
title = "Drosophila ovarian germline stem cell cytocensor projections dynamically receive and attenuate BMP signalling",
abstract = "In the Drosophila ovarian germline, BMP signals released by niche cells promotegermline stem cell (GSC) maintenance. Although BMP signaling is known to repress expression of a key differentiation factor, it remains unclear whether BMP-responsive transcription also contributes positively to GSC identity. Here, we identify the GSC transcriptome using RNA-seq, including the BMP-induced transcriptional network. Based on these data, we provide evidence that GSCs form two types of cellular projections. Genetic manipulation and live ex vivo imaging reveal that both classes of projection allow GSCs to access a reservoir of Dpp held away from the GSC-niche interface. Moreover, microtubule-rich projections, termed ‘cytocensors’, form downstream of BMP and have additional functionality, which is to attenuate BMP signaling. In this way cytocensors allow dynamic modulation of signal transduction to facilitate differentiation following GSC division. This ability of cytocensors to attenuate the signaling response expands the repertoire of functions associated with signaling projections.",
keywords = "BMP signalling, Drosophila, germline stem cell, cytoskeleton, niche, Dpp, Tkv, signalling filopodia",
author = "Scott Wilcockson and Hilary Ashe",
year = "2019",
month = "6",
day = "6",
doi = "10.1016/j.devcel.2019.05.020",
language = "English",
journal = "Developmental cell",
issn = "1534-5807",
publisher = "Cell Press",

}

RIS

TY - JOUR

T1 - Drosophila ovarian germline stem cell cytocensor projections dynamically receive and attenuate BMP signalling

AU - Wilcockson, Scott

AU - Ashe, Hilary

PY - 2019/6/6

Y1 - 2019/6/6

N2 - In the Drosophila ovarian germline, BMP signals released by niche cells promotegermline stem cell (GSC) maintenance. Although BMP signaling is known to repress expression of a key differentiation factor, it remains unclear whether BMP-responsive transcription also contributes positively to GSC identity. Here, we identify the GSC transcriptome using RNA-seq, including the BMP-induced transcriptional network. Based on these data, we provide evidence that GSCs form two types of cellular projections. Genetic manipulation and live ex vivo imaging reveal that both classes of projection allow GSCs to access a reservoir of Dpp held away from the GSC-niche interface. Moreover, microtubule-rich projections, termed ‘cytocensors’, form downstream of BMP and have additional functionality, which is to attenuate BMP signaling. In this way cytocensors allow dynamic modulation of signal transduction to facilitate differentiation following GSC division. This ability of cytocensors to attenuate the signaling response expands the repertoire of functions associated with signaling projections.

AB - In the Drosophila ovarian germline, BMP signals released by niche cells promotegermline stem cell (GSC) maintenance. Although BMP signaling is known to repress expression of a key differentiation factor, it remains unclear whether BMP-responsive transcription also contributes positively to GSC identity. Here, we identify the GSC transcriptome using RNA-seq, including the BMP-induced transcriptional network. Based on these data, we provide evidence that GSCs form two types of cellular projections. Genetic manipulation and live ex vivo imaging reveal that both classes of projection allow GSCs to access a reservoir of Dpp held away from the GSC-niche interface. Moreover, microtubule-rich projections, termed ‘cytocensors’, form downstream of BMP and have additional functionality, which is to attenuate BMP signaling. In this way cytocensors allow dynamic modulation of signal transduction to facilitate differentiation following GSC division. This ability of cytocensors to attenuate the signaling response expands the repertoire of functions associated with signaling projections.

KW - BMP signalling

KW - Drosophila

KW - germline stem cell

KW - cytoskeleton

KW - niche

KW - Dpp

KW - Tkv

KW - signalling filopodia

U2 - 10.1016/j.devcel.2019.05.020

DO - 10.1016/j.devcel.2019.05.020

M3 - Article

JO - Developmental cell

JF - Developmental cell

SN - 1534-5807

ER -