Direct Alkylation of Amines with Primary and Secondary Alcohols through Biocatalytic Hydrogen Borrowing

Research output: Contribution to journalArticle

  • External authors:
  • Sarah L Montgomery
  • Juan Mangas-Sanchez
  • Matthew P Thompson
  • Godwin A Aleku
  • Beatriz Dominguez


The reductive aminase from Aspergillus oryzae (AspRedAm) was combined with a single alcohol dehydrogenase (either metagenomic ADH-150, an ADH from Sphingobium yanoikuyae (SyADH), or a variant of the ADH from Thermoanaerobacter ethanolicus (TeSADH W110A)) in a redox-neutral cascade for the biocatalytic alkylation of amines using primary and secondary alcohols. Aliphatic and aromatic secondary amines were obtained in up to 99 % conversion, as well as chiral amines directly from the racemic alcohol precursors in up to >97 % ee, releasing water as the only byproduct.

Bibliographical metadata

Original languageEnglish
Pages (from-to)10491-10494
Number of pages4
JournalAngewandte Chemie (International ed. in English)
Issue number35
Early online date21 Jul 2017
Publication statusPublished - 16 Aug 2017

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