Development of an adaptable headspace sampling method for metabolic profiling of the fungal volatomeCitation formats

  • External authors:
  • Pavlos Geranios
  • Iain R White
  • Oluwasola Lawal
  • Tamara Nijsen
  • Royston Goodacre
  • Nick Read

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Development of an adaptable headspace sampling method for metabolic profiling of the fungal volatome. / Ahmed, Waqar M; Geranios, Pavlos; White, Iain R; Bromley, Michael; Lawal, Oluwasola; Nijsen, Tamara; Goodacre, Royston; Read, Nick; Fowler, Stephen J.

In: The Analyst, 2018.

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Ahmed, Waqar M ; Geranios, Pavlos ; White, Iain R ; Bromley, Michael ; Lawal, Oluwasola ; Nijsen, Tamara ; Goodacre, Royston ; Read, Nick ; Fowler, Stephen J. / Development of an adaptable headspace sampling method for metabolic profiling of the fungal volatome. In: The Analyst. 2018.

Bibtex

@article{0cc47d18ad1a4a5b96bcb5a5189d7e39,
title = "Development of an adaptable headspace sampling method for metabolic profiling of the fungal volatome",
abstract = "Pulmonary aspergillosis can cause serious complications in people with a suppressed immune system. Volatile metabolites emitted by Aspergillus spp. have shown promise for early detection of pathogenicity. However, volatile profiles require further research, as effective headspace analysis methods are required for extended chemical coverage of the volatome; in terms of both very volatile and semi-volatile compounds. In this study, we describe a novel adaptable sampling method in which fungal headspace samples can be sampled continuously throughout a defined time period using both active (pumped) and passive (diffusive) methods, with the capability for samples to be stored for later off-line analysis. For this method we utilise thermal desorption-gas chromatography-mass spectrometry to generate volatile metabolic profiles using Aspergillus fumigatus as the model organism. Several known fungal-specific volatiles associated with secondary metabolite biosynthesis (including α-pinene, camphene, limonene, and several sesquiterpenes) were identified. A comparison between the wild-type A. fumigatus with a phosphopantetheinyl transferase null mutant strain (ΔpptA) that is compromised in secondary metabolite synthesis, revealed reduced production of sesquiterpenes. We also showed the lack of terpene compounds production during the early growth phase, whilst pyrazines were identified in both early and late growth phases. We have demonstrated that the fungal volatome is dynamic and it is therefore critically necessary to sample the headspace across several time periods using a combination of active and passive sampling techniques to analyse and understand this dynamism.",
author = "Ahmed, {Waqar M} and Pavlos Geranios and White, {Iain R} and Michael Bromley and Oluwasola Lawal and Tamara Nijsen and Royston Goodacre and Nick Read and Fowler, {Stephen J}",
year = "2018",
doi = "10.1039/C8AN00841H",
language = "English",
journal = "Analyst",
issn = "0003-2654",
publisher = "Royal Society of Chemistry",

}

RIS

TY - JOUR

T1 - Development of an adaptable headspace sampling method for metabolic profiling of the fungal volatome

AU - Ahmed, Waqar M

AU - Geranios, Pavlos

AU - White, Iain R

AU - Bromley, Michael

AU - Lawal, Oluwasola

AU - Nijsen, Tamara

AU - Goodacre, Royston

AU - Read, Nick

AU - Fowler, Stephen J

PY - 2018

Y1 - 2018

N2 - Pulmonary aspergillosis can cause serious complications in people with a suppressed immune system. Volatile metabolites emitted by Aspergillus spp. have shown promise for early detection of pathogenicity. However, volatile profiles require further research, as effective headspace analysis methods are required for extended chemical coverage of the volatome; in terms of both very volatile and semi-volatile compounds. In this study, we describe a novel adaptable sampling method in which fungal headspace samples can be sampled continuously throughout a defined time period using both active (pumped) and passive (diffusive) methods, with the capability for samples to be stored for later off-line analysis. For this method we utilise thermal desorption-gas chromatography-mass spectrometry to generate volatile metabolic profiles using Aspergillus fumigatus as the model organism. Several known fungal-specific volatiles associated with secondary metabolite biosynthesis (including α-pinene, camphene, limonene, and several sesquiterpenes) were identified. A comparison between the wild-type A. fumigatus with a phosphopantetheinyl transferase null mutant strain (ΔpptA) that is compromised in secondary metabolite synthesis, revealed reduced production of sesquiterpenes. We also showed the lack of terpene compounds production during the early growth phase, whilst pyrazines were identified in both early and late growth phases. We have demonstrated that the fungal volatome is dynamic and it is therefore critically necessary to sample the headspace across several time periods using a combination of active and passive sampling techniques to analyse and understand this dynamism.

AB - Pulmonary aspergillosis can cause serious complications in people with a suppressed immune system. Volatile metabolites emitted by Aspergillus spp. have shown promise for early detection of pathogenicity. However, volatile profiles require further research, as effective headspace analysis methods are required for extended chemical coverage of the volatome; in terms of both very volatile and semi-volatile compounds. In this study, we describe a novel adaptable sampling method in which fungal headspace samples can be sampled continuously throughout a defined time period using both active (pumped) and passive (diffusive) methods, with the capability for samples to be stored for later off-line analysis. For this method we utilise thermal desorption-gas chromatography-mass spectrometry to generate volatile metabolic profiles using Aspergillus fumigatus as the model organism. Several known fungal-specific volatiles associated with secondary metabolite biosynthesis (including α-pinene, camphene, limonene, and several sesquiterpenes) were identified. A comparison between the wild-type A. fumigatus with a phosphopantetheinyl transferase null mutant strain (ΔpptA) that is compromised in secondary metabolite synthesis, revealed reduced production of sesquiterpenes. We also showed the lack of terpene compounds production during the early growth phase, whilst pyrazines were identified in both early and late growth phases. We have demonstrated that the fungal volatome is dynamic and it is therefore critically necessary to sample the headspace across several time periods using a combination of active and passive sampling techniques to analyse and understand this dynamism.

U2 - 10.1039/C8AN00841H

DO - 10.1039/C8AN00841H

M3 - Article

JO - Analyst

JF - Analyst

SN - 0003-2654

ER -