Development of a Solid Phase Array Assay for the Screening of Galactose Oxidase Activity and for Fast Identification of Inhibitors

Research output: Contribution to journalArticle

  • External authors:
  • Martin J. Weissenborn
  • Damien P Debecker
  • Samuel Golten
  • Bruno Linclau

Abstract

Galactose oxidase catalyses the highly selective oxidation of terminal galactosides on a wide range of natural glycoconjugates and has found wide applications in biotechnology, particularly in biocatalysis. Here we show that the enzyme is able to oxidise solid gold-SAM surfaces functionalised with galactose. This array platform allows for the fast identification of galactose oxidase substrates and inhibitors from a library of deoxy-fluorinated sugars using MALDI-Tof spectrometry as a label–free read-out method. In addition, the enzymatic reaction enables for the in situ activation of sugar-coated surfaces to bioorthogonal aldehydes, which can be used for subsequent chemical modifications.

Bibliographical metadata

Original languageEnglish
Pages (from-to)742-746
Number of pages5
JournalProtein and Peptide Letters
Volume24
Issue number8
DOIs
Publication statusPublished - 2017