Development of a high-throughput screening method for racemase activity and its application to the identification of alanine racemase variants with activity towards l-arginineCitation formats

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Development of a high-throughput screening method for racemase activity and its application to the identification of alanine racemase variants with activity towards l-arginine. / Willies, Simon C.; White, Jemma L.; Turner, Nicholas J.

In: Tetrahedron, Vol. 68, No. 37, 16.09.2012, p. 7564-7567.

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@article{e8dc0f4210b74650a16678b0b818cce0,
title = "Development of a high-throughput screening method for racemase activity and its application to the identification of alanine racemase variants with activity towards l-arginine",
abstract = "A high-throughput solid phase screening method has been developed for alanine racemase. Conversion of an l-amino acid to the d-amino acid is detected by a d-amino acid oxidase, which is utilised as a reporter enzyme along with horseradish peroxidase and a colourimetric dye. This system has successfully been used to identify a mutant with increased activity towards arginine. Kinetic analysis and modelling studies have been performed to understand the effect of the mutations found. {\circledC} 2012 Elsevier Ltd. All rights reserved.",
keywords = "Amino acid, Biocatalysis, High-throughput screening, Protein engineering, Racemase",
author = "Willies, {Simon C.} and White, {Jemma L.} and Turner, {Nicholas J.}",
year = "2012",
month = "9",
day = "16",
doi = "10.1016/j.tet.2012.06.062",
language = "English",
volume = "68",
pages = "7564--7567",
journal = "Tetrahedron",
issn = "0040-4020",
publisher = "Elsevier BV",
number = "37",

}

RIS

TY - JOUR

T1 - Development of a high-throughput screening method for racemase activity and its application to the identification of alanine racemase variants with activity towards l-arginine

AU - Willies, Simon C.

AU - White, Jemma L.

AU - Turner, Nicholas J.

PY - 2012/9/16

Y1 - 2012/9/16

N2 - A high-throughput solid phase screening method has been developed for alanine racemase. Conversion of an l-amino acid to the d-amino acid is detected by a d-amino acid oxidase, which is utilised as a reporter enzyme along with horseradish peroxidase and a colourimetric dye. This system has successfully been used to identify a mutant with increased activity towards arginine. Kinetic analysis and modelling studies have been performed to understand the effect of the mutations found. © 2012 Elsevier Ltd. All rights reserved.

AB - A high-throughput solid phase screening method has been developed for alanine racemase. Conversion of an l-amino acid to the d-amino acid is detected by a d-amino acid oxidase, which is utilised as a reporter enzyme along with horseradish peroxidase and a colourimetric dye. This system has successfully been used to identify a mutant with increased activity towards arginine. Kinetic analysis and modelling studies have been performed to understand the effect of the mutations found. © 2012 Elsevier Ltd. All rights reserved.

KW - Amino acid

KW - Biocatalysis

KW - High-throughput screening

KW - Protein engineering

KW - Racemase

U2 - 10.1016/j.tet.2012.06.062

DO - 10.1016/j.tet.2012.06.062

M3 - Article

VL - 68

SP - 7564

EP - 7567

JO - Tetrahedron

JF - Tetrahedron

SN - 0040-4020

IS - 37

ER -