Detection of human cytomegalovirus antigenaemia: A rapid diagnostic technique for predicting cytomegalovirus infection/pneumonitis in lung and heart transplant recipientsCitation formats

  • Authors:
  • J. J. Egan
  • L. Barber
  • J. Lomax
  • A. Fox
  • N. Yonan
  • And 7 others
  • External authors:
  • A. N. Rahman
  • C. S. Campbell
  • A. K. Deiraniya
  • K. B. Carroll
  • J. Craske
  • A. Turner
  • A. A. Woodcock

Standard

Detection of human cytomegalovirus antigenaemia: A rapid diagnostic technique for predicting cytomegalovirus infection/pneumonitis in lung and heart transplant recipients. / Egan, J. J.; Barber, L.; Lomax, J.; Fox, A.; Yonan, N.; Rahman, A. N.; Campbell, C. S.; Deiraniya, A. K.; Carroll, K. B.; Craske, J.; Turner, A.; Woodcock, A. A.

In: Thorax, Vol. 50, No. 1, 1995, p. 9-13.

Research output: Contribution to journalArticle

Harvard

Egan, JJ, Barber, L, Lomax, J, Fox, A, Yonan, N, Rahman, AN, Campbell, CS, Deiraniya, AK, Carroll, KB, Craske, J, Turner, A & Woodcock, AA 1995, 'Detection of human cytomegalovirus antigenaemia: A rapid diagnostic technique for predicting cytomegalovirus infection/pneumonitis in lung and heart transplant recipients' Thorax, vol. 50, no. 1, pp. 9-13.

APA

Egan, J. J., Barber, L., Lomax, J., Fox, A., Yonan, N., Rahman, A. N., ... Woodcock, A. A. (1995). Detection of human cytomegalovirus antigenaemia: A rapid diagnostic technique for predicting cytomegalovirus infection/pneumonitis in lung and heart transplant recipients. Thorax, 50(1), 9-13.

Vancouver

Author

Egan, J. J. ; Barber, L. ; Lomax, J. ; Fox, A. ; Yonan, N. ; Rahman, A. N. ; Campbell, C. S. ; Deiraniya, A. K. ; Carroll, K. B. ; Craske, J. ; Turner, A. ; Woodcock, A. A. / Detection of human cytomegalovirus antigenaemia: A rapid diagnostic technique for predicting cytomegalovirus infection/pneumonitis in lung and heart transplant recipients. In: Thorax. 1995 ; Vol. 50, No. 1. pp. 9-13.

Bibtex

@article{cd51839959974cf7be51e8bd9b3881f6,
title = "Detection of human cytomegalovirus antigenaemia: A rapid diagnostic technique for predicting cytomegalovirus infection/pneumonitis in lung and heart transplant recipients",
abstract = "Background - New rapid diagnostic techniques offer the opportunity of early diagnosis of human cytomegalovirus (CMV) infection in immunocompromised patients at risk of developing CMV disease. The use of human CMV antigenaemia as a predictor of clinical CMV infection and disease in lung and heart transplant recipients was studied prospectively. Methods - Twenty three heart and nine lung transplant recipients who survived 40 days were observed by standard CMV surveillance with serological testing, culture, and by sequential testing for CMV antigenaemia. CMV antigenaemia testing is a rapid and quantifiable technique in which a viral lower matrix protein is detected in cytospin preparations of peripheral blood polymorphonuclear leucocytes (PMNLs) by immunofluorescent staining. Results - Eleven patients developed CMV infection and five developed CMV disease (four pneumonitis, one duodenitis). These clinical events occurred at a median of 65 days following transplantation. CMV antigenaemia occurred in 17 patients at a median of 35 days following transplantation. Detection of CMV antigenaemia had a sensitivity of 100{\%}, a specificity of 93.7{\%}, and a positive predictive value of 94.1{\%} for CMV related illness. CMV antigenaemia was positive at a significant interval before the clinical event. High levels of CMV antigenaemia (>50 CMV antigen positive cells/2 x 105 PMNLs) occurred in 11 patients and five of these developed disease. CMV antigenaemia of >50 CMV antigen positive cells/2x105 PMNLs had a positive predictive value of 45.5{\%} for disease but a negative predictive value of 100{\%}. Patients with disease had higher levels of antigenaemia than those without disease. Conclusions - CMV antigenaemia is a rapid diagnostic technique which can identify patients likely to develop CMV disease, potentially allowing early treatment.",
keywords = "Cytomegalovirus, Heart transplantation, Lung transplantation",
author = "Egan, {J. J.} and L. Barber and J. Lomax and A. Fox and N. Yonan and Rahman, {A. N.} and Campbell, {C. S.} and Deiraniya, {A. K.} and Carroll, {K. B.} and J. Craske and A. Turner and Woodcock, {A. A.}",
year = "1995",
language = "English",
volume = "50",
pages = "9--13",
journal = "Thorax",
issn = "0040-6376",
publisher = "B M J Group",
number = "1",

}

RIS

TY - JOUR

T1 - Detection of human cytomegalovirus antigenaemia: A rapid diagnostic technique for predicting cytomegalovirus infection/pneumonitis in lung and heart transplant recipients

AU - Egan, J. J.

AU - Barber, L.

AU - Lomax, J.

AU - Fox, A.

AU - Yonan, N.

AU - Rahman, A. N.

AU - Campbell, C. S.

AU - Deiraniya, A. K.

AU - Carroll, K. B.

AU - Craske, J.

AU - Turner, A.

AU - Woodcock, A. A.

PY - 1995

Y1 - 1995

N2 - Background - New rapid diagnostic techniques offer the opportunity of early diagnosis of human cytomegalovirus (CMV) infection in immunocompromised patients at risk of developing CMV disease. The use of human CMV antigenaemia as a predictor of clinical CMV infection and disease in lung and heart transplant recipients was studied prospectively. Methods - Twenty three heart and nine lung transplant recipients who survived 40 days were observed by standard CMV surveillance with serological testing, culture, and by sequential testing for CMV antigenaemia. CMV antigenaemia testing is a rapid and quantifiable technique in which a viral lower matrix protein is detected in cytospin preparations of peripheral blood polymorphonuclear leucocytes (PMNLs) by immunofluorescent staining. Results - Eleven patients developed CMV infection and five developed CMV disease (four pneumonitis, one duodenitis). These clinical events occurred at a median of 65 days following transplantation. CMV antigenaemia occurred in 17 patients at a median of 35 days following transplantation. Detection of CMV antigenaemia had a sensitivity of 100%, a specificity of 93.7%, and a positive predictive value of 94.1% for CMV related illness. CMV antigenaemia was positive at a significant interval before the clinical event. High levels of CMV antigenaemia (>50 CMV antigen positive cells/2 x 105 PMNLs) occurred in 11 patients and five of these developed disease. CMV antigenaemia of >50 CMV antigen positive cells/2x105 PMNLs had a positive predictive value of 45.5% for disease but a negative predictive value of 100%. Patients with disease had higher levels of antigenaemia than those without disease. Conclusions - CMV antigenaemia is a rapid diagnostic technique which can identify patients likely to develop CMV disease, potentially allowing early treatment.

AB - Background - New rapid diagnostic techniques offer the opportunity of early diagnosis of human cytomegalovirus (CMV) infection in immunocompromised patients at risk of developing CMV disease. The use of human CMV antigenaemia as a predictor of clinical CMV infection and disease in lung and heart transplant recipients was studied prospectively. Methods - Twenty three heart and nine lung transplant recipients who survived 40 days were observed by standard CMV surveillance with serological testing, culture, and by sequential testing for CMV antigenaemia. CMV antigenaemia testing is a rapid and quantifiable technique in which a viral lower matrix protein is detected in cytospin preparations of peripheral blood polymorphonuclear leucocytes (PMNLs) by immunofluorescent staining. Results - Eleven patients developed CMV infection and five developed CMV disease (four pneumonitis, one duodenitis). These clinical events occurred at a median of 65 days following transplantation. CMV antigenaemia occurred in 17 patients at a median of 35 days following transplantation. Detection of CMV antigenaemia had a sensitivity of 100%, a specificity of 93.7%, and a positive predictive value of 94.1% for CMV related illness. CMV antigenaemia was positive at a significant interval before the clinical event. High levels of CMV antigenaemia (>50 CMV antigen positive cells/2 x 105 PMNLs) occurred in 11 patients and five of these developed disease. CMV antigenaemia of >50 CMV antigen positive cells/2x105 PMNLs had a positive predictive value of 45.5% for disease but a negative predictive value of 100%. Patients with disease had higher levels of antigenaemia than those without disease. Conclusions - CMV antigenaemia is a rapid diagnostic technique which can identify patients likely to develop CMV disease, potentially allowing early treatment.

KW - Cytomegalovirus

KW - Heart transplantation

KW - Lung transplantation

M3 - Article

VL - 50

SP - 9

EP - 13

JO - Thorax

JF - Thorax

SN - 0040-6376

IS - 1

ER -