Derivative manipulation in the structure solution of the integral membrane LH2 complex

Research output: Contribution to journalArticlepeer-review

  • External authors:
  • G. McDermott
  • A. A. Freer
  • M. Z. Papiz
  • A. M. Lawless
  • R. J. Cogdell
  • N. W. Isaacs

Abstract

The structure of the peripheral light-harvesting complex from Rhodopseudomonas acidophila strain 10050 was determined by multiple isomorphous replacement methods. The derivatization of the crystals was augmented by the addition of a backsoaking stage. The soak/backsoaked data comparison had greater isomorphism and showed simpler Patterson maps than the standard native/soak comparison. Amplitudes from the derivatized then backsoaked crystals and from the derivatized crystals were compared in order to extract a subset of heavy-atom sites. Using this information, the full array of sites were found from a derivative/native comparison, eventually leading to excellent electron-density maps.

Bibliographical metadata

Original languageEnglish
Pages (from-to)1428-1431
Number of pages3
JournalActa Crystallographica Section D: Biological Crystallography
Volume55
Issue number8
DOIs
Publication statusPublished - Aug 1999