Defective interferon-gamma production is common in chronic pulmonary aspergillosisCitation formats

  • External authors:
  • Rola Hashad
  • Dinakantha S. Kumararatne
  • Lourdes Ceron-Gutierrez
  • Gabriela Barcenas-Morales
  • Chris Harris
  • Rainer Doffinger

Standard

Defective interferon-gamma production is common in chronic pulmonary aspergillosis. / Colombo, Stefano A P; Hashad, Rola; Denning, David W et al.

In: The Journal of Infectious Diseases, 25.11.2021.

Research output: Contribution to journalArticlepeer-review

Harvard

Colombo, SAP, Hashad, R, Denning, DW, Kumararatne, DS, Ceron-Gutierrez, L, Barcenas-Morales, G, MacDonald, AS, Harris, C, Doffinger, R & Kosmidis, C 2021, 'Defective interferon-gamma production is common in chronic pulmonary aspergillosis', The Journal of Infectious Diseases.

APA

Colombo, S. A. P., Hashad, R., Denning, D. W., Kumararatne, D. S., Ceron-Gutierrez, L., Barcenas-Morales, G., MacDonald, A. S., Harris, C., Doffinger, R., & Kosmidis, C. (Accepted/In press). Defective interferon-gamma production is common in chronic pulmonary aspergillosis. The Journal of Infectious Diseases.

Vancouver

Colombo SAP, Hashad R, Denning DW, Kumararatne DS, Ceron-Gutierrez L, Barcenas-Morales G et al. Defective interferon-gamma production is common in chronic pulmonary aspergillosis. The Journal of Infectious Diseases. 2021 Nov 25.

Author

Bibtex

@article{2d82365abe074906bea4f796d4129d9a,
title = "Defective interferon-gamma production is common in chronic pulmonary aspergillosis",
abstract = "Background: Immune defects in chronic pulmonary aspergillosis (CPA) are poorly characterised. We compared peripheral blood cytokine profiles in patients with CPA vs healthy controls and explored the relationship with disease severity.Methods: Interferon-gamma (IFNγ), IL-17, TNFα, IL-6, IL-12 and IL-10 were measured after in vitro stimulation of whole blood with lipopolysaccharide (LPS), phytohaemagglutinin (PHA), β-glucan, zymosan (ZYM), IL-12 or IL-18, and combinations. Clinical parameters and mortality were correlated with cytokine production. Results: Cytokine profiles were evaluated in 133 patients (57.1% male, mean age 61 years). In comparison to controls, patients with CPA had significantly reduced production of IFNγ in response to stimulation with β-glucan+IL-12 (312 vs 988 pg/ml), LPS+IL-12 (252 vs 1033 pg/ml), ZYM+IL-12 (996 vs 2347 pg/ml), and IL-18+IL-12 (7193 vs 12330 pg/ml). Age >60 (p=0.05, HR 1.71, 95%CI 1.00-2.91) and COPD (p=0.039, HR 1.69, 95%CI 1.03-2.78) were associated with worse survival, whereas high IFNγ production in response to beta-glucan+IL-12 stimulation (p=0.026, HR 0.48, 95%CI 0.25-0.92) was associated with reduced mortality. Conclusion: Patients with CPA show impaired IFNγ production in peripheral blood in response to stimuli. Defective IFNγ production ability correlates with worse outcomes. Immunotherapy with IFNγ could be beneficial for patients showing impaired IFNγ production in CPA.Keywords: Chronic pulmonary aspergillosis, pathogenesis, cytokines, interferon-gamma, IL-17A, immunotherapy ",
author = "Colombo, {Stefano A P} and Rola Hashad and Denning, {David W} and Kumararatne, {Dinakantha S.} and Lourdes Ceron-Gutierrez and Gabriela Barcenas-Morales and MacDonald, {Andrew S} and Chris Harris and Rainer Doffinger and Chris Kosmidis",
year = "2021",
month = nov,
day = "25",
language = "English",
journal = "Journal of INfectious Diseases",
issn = "1537-6613",
publisher = "Oxford University Press ",

}

RIS

TY - JOUR

T1 - Defective interferon-gamma production is common in chronic pulmonary aspergillosis

AU - Colombo, Stefano A P

AU - Hashad, Rola

AU - Denning, David W

AU - Kumararatne, Dinakantha S.

AU - Ceron-Gutierrez, Lourdes

AU - Barcenas-Morales, Gabriela

AU - MacDonald, Andrew S

AU - Harris, Chris

AU - Doffinger, Rainer

AU - Kosmidis, Chris

PY - 2021/11/25

Y1 - 2021/11/25

N2 - Background: Immune defects in chronic pulmonary aspergillosis (CPA) are poorly characterised. We compared peripheral blood cytokine profiles in patients with CPA vs healthy controls and explored the relationship with disease severity.Methods: Interferon-gamma (IFNγ), IL-17, TNFα, IL-6, IL-12 and IL-10 were measured after in vitro stimulation of whole blood with lipopolysaccharide (LPS), phytohaemagglutinin (PHA), β-glucan, zymosan (ZYM), IL-12 or IL-18, and combinations. Clinical parameters and mortality were correlated with cytokine production. Results: Cytokine profiles were evaluated in 133 patients (57.1% male, mean age 61 years). In comparison to controls, patients with CPA had significantly reduced production of IFNγ in response to stimulation with β-glucan+IL-12 (312 vs 988 pg/ml), LPS+IL-12 (252 vs 1033 pg/ml), ZYM+IL-12 (996 vs 2347 pg/ml), and IL-18+IL-12 (7193 vs 12330 pg/ml). Age >60 (p=0.05, HR 1.71, 95%CI 1.00-2.91) and COPD (p=0.039, HR 1.69, 95%CI 1.03-2.78) were associated with worse survival, whereas high IFNγ production in response to beta-glucan+IL-12 stimulation (p=0.026, HR 0.48, 95%CI 0.25-0.92) was associated with reduced mortality. Conclusion: Patients with CPA show impaired IFNγ production in peripheral blood in response to stimuli. Defective IFNγ production ability correlates with worse outcomes. Immunotherapy with IFNγ could be beneficial for patients showing impaired IFNγ production in CPA.Keywords: Chronic pulmonary aspergillosis, pathogenesis, cytokines, interferon-gamma, IL-17A, immunotherapy

AB - Background: Immune defects in chronic pulmonary aspergillosis (CPA) are poorly characterised. We compared peripheral blood cytokine profiles in patients with CPA vs healthy controls and explored the relationship with disease severity.Methods: Interferon-gamma (IFNγ), IL-17, TNFα, IL-6, IL-12 and IL-10 were measured after in vitro stimulation of whole blood with lipopolysaccharide (LPS), phytohaemagglutinin (PHA), β-glucan, zymosan (ZYM), IL-12 or IL-18, and combinations. Clinical parameters and mortality were correlated with cytokine production. Results: Cytokine profiles were evaluated in 133 patients (57.1% male, mean age 61 years). In comparison to controls, patients with CPA had significantly reduced production of IFNγ in response to stimulation with β-glucan+IL-12 (312 vs 988 pg/ml), LPS+IL-12 (252 vs 1033 pg/ml), ZYM+IL-12 (996 vs 2347 pg/ml), and IL-18+IL-12 (7193 vs 12330 pg/ml). Age >60 (p=0.05, HR 1.71, 95%CI 1.00-2.91) and COPD (p=0.039, HR 1.69, 95%CI 1.03-2.78) were associated with worse survival, whereas high IFNγ production in response to beta-glucan+IL-12 stimulation (p=0.026, HR 0.48, 95%CI 0.25-0.92) was associated with reduced mortality. Conclusion: Patients with CPA show impaired IFNγ production in peripheral blood in response to stimuli. Defective IFNγ production ability correlates with worse outcomes. Immunotherapy with IFNγ could be beneficial for patients showing impaired IFNγ production in CPA.Keywords: Chronic pulmonary aspergillosis, pathogenesis, cytokines, interferon-gamma, IL-17A, immunotherapy

M3 - Article

JO - Journal of INfectious Diseases

JF - Journal of INfectious Diseases

SN - 1537-6613

ER -