Core Fermentation (CoFe) granules focus coordinated glycolytic mRNA localization and translation to fuel glucose fermentation

Research output: Contribution to journalArticlepeer-review

  • External authors:
  • Fabián Morales Polanco
  • Christian Bates
  • Jennifer Lui
  • Joe Casson
  • Clara Solari
  • Mariavittoria Pizzinga
  • Gabbie Forte
  • Claire Griffin
  • Kirsten Garner
  • Harriet Burt
  • Hannah Dixon
  • Paula Portela


Glycolysis is a fundamental metabolic pathway for glucose catabolism across biology, and glycolytic enzymes are amongst the most abundant proteins in cells. Their expression at such levels provides a particular challenge. Here we demonstrate that the glycolytic mRNAs are localized to granules in yeast and human cells. Detailed live cell and smFISH studies in yeast show that the mRNAs are actively translated in granules, and this translation appears critical for the localization. Furthermore, this arrangement is likely to facilitate the higher level organisation and control of the glycolytic pathway. Indeed, the degree of fermentation required by cells is intrinsically connected to the extent of mRNA localization to granules. On this basis, we term these granules, Core Fermentation (CoFe) granules; they appear to represent translation factories allowing high-level co-ordinated enzyme synthesis for a critical metabolic pathway.

Bibliographical metadata

Original languageEnglish
Pages (from-to)102069
Publication statusPublished - 19 Feb 2021