Chemical genetics screening reveals KIAA1363 as a cytokine-lowering target.

Research output: Research - peer-reviewArticle

  • Authors:
  • Devon M Hunerdosse
  • Patrick J Morris
  • David K Miyamoto
  • Karl J Fisher
  • Leslie A Bateman
  • And 3 others
  • External authors:
  • Jonathan R Ghazaleh
  • Sharon Zhong
  • Daniel K Nomura

Abstract

Inflammation is a hallmark of many human diseases, including pain, arthritis, atherosclerosis, obesity and diabetes, cancer, and neurodegenerative diseases. Although there are several successfully marketed small molecules anti-inflammatory drugs such as cyclooxygenase inhibitors and glucocorticoids, many of these compounds are also associated with various adverse cardiovascular or immunosuppressive side effects. Thus, identifying novel anti-inflammatory small molecules and their targets is critical for developing safer and more effective next-generation treatment strategies for inflammatory diseases. Here, we have conducted a chemical genetics screen to identify small molecules that suppress the release of the inflammatory cytokine TNFα from stimulated macrophages. We have used an enzyme class-directed chemical library for our screening efforts to facilitate subsequent target identification using activity-based protein profiling (ABPP). Using this strategy, we have found that KIAA1363 is a novel target for lowering key pro-inflammatory cytokines through affecting key ether lipid metabolism pathways. Our study highlights the application of combining chemical genetics with chemoproteomic and metabolomic approaches toward identifying and characterizing anti-inflammatory smal molecules and their targets.

Bibliographical metadata

Original languageEnglish
JournalACS chemical biology
Volume9
Issue number12
DOIs
StatePublished - 19 Dec 2014