Characterizing monoclonal antibody formulations in arginine glutamate solutions using 1H NMR spectroscopy

Research output: Contribution to journalArticle

  • Authors:
  • Priscilla Kheddo
  • Matthew Cliff
  • Shahid Uddin
  • Christopher F van der Walle
  • Alexander Golovanov


Assessing how excipients affect the self-association of monoclonal antibodies (mAbs) requires informative and direct in situ measurements for highly concentrated solutions, without sample dilution or perturbation. This study explores the application of solution nuclear magnetic resonance (NMR) spectroscopy for characterization of typical mAb behavior in formulations containing arginine glutamate. The data show that the analysis of signal intensities in 1D 1H NMR spectra, when compensated for changes in buffer viscosity, is invaluable for identifying conditions where protein-protein interactions are minimized. NMR-derived molecular translational diffusion rates for concentrated solutions are less useful than transverse relaxation rates as parameters defining optimal formulation. Furthermore, NMR reports on the solution viscosity and mAb aggregation during accelerated stability study assessment, generating data consistent with that acquired by size-exclusion chromatography. The methodology developed here offers NMR spectroscopy as a new tool providing complementary information useful to formulation development of mAbs and other large therapeutic proteins.

Bibliographical metadata

Original languageEnglish
Pages (from-to)1245-1258
Issue number7
StatePublished - 11 Aug 2016