Characterization of a conditional interleukin-1 receptor 1 mouse mutant using the Cre/LoxP system

Research output: Contribution to journalArticle

  • External authors:
  • Wesam Abdulaal
  • Catherine Walker
  • Ryan Costello
  • Ilgiz A Mufazalov
  • Athina Papaemmanouil
  • Ari Waisman

Abstract

IL-1 is a key cytokine known to drive chronic inflammation and to regulate many physiological, immunological and neuroimmunological responses via actions on diverse cell types of the body. To determine the mechanisms of IL-1 actions as part of the inflammatory response in vivo, we generated a conditional IL-1 receptor 1 (IL-1R1) mouse mutant using the Cre/LoxP system (IL-1R1(fl/fl) ). In the mutant generated, exon 5, which encodes part of the extracellular binding region of the receptor, is flanked by LoxP sites, thereby inactivating the two previously described functional IL-1R1 gene transcripts after Cre-mediated recombination. Using keratin 14-Cre driver mice, new IL-1R1 deficient (-/-) mice were subsequently generated, in which all signaling IL-1 receptor isoforms are deleted ubiquitously. Furthermore, using vav-iCre driver mice, we deleted IL-1 receptor isoforms in the haematopoietic system. In these mice, we show that both the IL-17 and IL-22 cytokine response is reduced, when mice are challenged by the helminth Trichuris muris. We are currently crossing IL-1R1(fl/fl) mice with different Cre-expressing mice in order to study mechanisms of acute and chronic inflammatory diseases. This article is protected by copyright. All rights reserved.

Bibliographical metadata

Original languageEnglish
Pages (from-to)912-918
Number of pages7
JournalEuropean journal of immunology
Volume46
Issue number4
Early online date18 Jan 2016
DOIs
Publication statusPublished - Apr 2016

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