Cellular uptake and cytotoxic impact of chemically functionalized and polymer-coated carbon nanotubesCitation formats

  • External authors:
  • Hanene Ali-Boucetta
  • Khuloud T. Al-Jamal
  • Karin H. Müller
  • Shouping Li
  • Alexandra E. Porter
  • Ayad Eddaoudi
  • Maurizio Prato
  • Alberto Bianco

Standard

Cellular uptake and cytotoxic impact of chemically functionalized and polymer-coated carbon nanotubes. / Ali-Boucetta, Hanene; Al-Jamal, Khuloud T.; Müller, Karin H.; Li, Shouping; Porter, Alexandra E.; Eddaoudi, Ayad; Prato, Maurizio; Bianco, Alberto; Kostarelos, Kostas.

In: Small, Vol. 7, No. 22, 18.11.2011, p. 3230-3238.

Research output: Contribution to journalArticlepeer-review

Harvard

Ali-Boucetta, H, Al-Jamal, KT, Müller, KH, Li, S, Porter, AE, Eddaoudi, A, Prato, M, Bianco, A & Kostarelos, K 2011, 'Cellular uptake and cytotoxic impact of chemically functionalized and polymer-coated carbon nanotubes', Small, vol. 7, no. 22, pp. 3230-3238. https://doi.org/10.1002/smll.201101004

APA

Ali-Boucetta, H., Al-Jamal, K. T., Müller, K. H., Li, S., Porter, A. E., Eddaoudi, A., Prato, M., Bianco, A., & Kostarelos, K. (2011). Cellular uptake and cytotoxic impact of chemically functionalized and polymer-coated carbon nanotubes. Small, 7(22), 3230-3238. https://doi.org/10.1002/smll.201101004

Vancouver

Ali-Boucetta H, Al-Jamal KT, Müller KH, Li S, Porter AE, Eddaoudi A et al. Cellular uptake and cytotoxic impact of chemically functionalized and polymer-coated carbon nanotubes. Small. 2011 Nov 18;7(22):3230-3238. https://doi.org/10.1002/smll.201101004

Author

Ali-Boucetta, Hanene ; Al-Jamal, Khuloud T. ; Müller, Karin H. ; Li, Shouping ; Porter, Alexandra E. ; Eddaoudi, Ayad ; Prato, Maurizio ; Bianco, Alberto ; Kostarelos, Kostas. / Cellular uptake and cytotoxic impact of chemically functionalized and polymer-coated carbon nanotubes. In: Small. 2011 ; Vol. 7, No. 22. pp. 3230-3238.

Bibtex

@article{122b603660a74caba101b4d49c43e9d3,
title = "Cellular uptake and cytotoxic impact of chemically functionalized and polymer-coated carbon nanotubes",
abstract = "The impact of nanomaterials such as carbon nanotubes on biological matter is a topic of increasing interest and concern and requires a multifaceted approach to be resolved. A modified cytotoxic (lactate dehydrogenase (LDH)) assay is developed in an attempt to offer a valid and reliable methodology for screening carbon nanotube toxicity in vitro. Two of the most widely used types of surface-modified multiwalled carbon nanotubes (MWNTs) are tested: ammonium-functionalized MWNTs (MWNT-NH 3 +) and Pluronic F127 coated MWNTs (MWNT:F127). Chemically functionalized MWNTs show significantly greater cellular uptake into lung epithelial A549 cells compared to the non-covalently Pluronic F127-coated MWNTs. In spite of this, MWNT:F127 exhibit enhanced cytotoxicity according to the modified LDH assay. The validity of the modified LDH assay is further validated by direct comparison with other less reliable or accurate cytotoxicity assays. These findings indicate the reliability of the modified LDH assay as a screening tool to assess carbon nanotube cytotoxicity and illustrate that high levels of carbon nanotube cellular internalization do not necessarily lead to adverse responses. A modified cytotoxic (LDH) assay is developed in an attempt to offer a valid and reliable methodology for screening carbon nanotube (CNT) toxicity in vitro. Two types of CNT are screened using this assay and the results are compared to other commonly used assays. This assay is a promising tool, particularly in a field eager for reliable in vitro cytotoxicity assays. {\textcopyright} 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.",
keywords = "cell internalization, cytotoxicity, nanotoxicology, nanotubes",
author = "Hanene Ali-Boucetta and Al-Jamal, {Khuloud T.} and M{\"u}ller, {Karin H.} and Shouping Li and Porter, {Alexandra E.} and Ayad Eddaoudi and Maurizio Prato and Alberto Bianco and Kostas Kostarelos",
note = "Times Cited: 9 0 9",
year = "2011",
month = nov,
day = "18",
doi = "10.1002/smll.201101004",
language = "English",
volume = "7",
pages = "3230--3238",
journal = "Small",
issn = "1613-6810",
publisher = "John Wiley & Sons Ltd",
number = "22",

}

RIS

TY - JOUR

T1 - Cellular uptake and cytotoxic impact of chemically functionalized and polymer-coated carbon nanotubes

AU - Ali-Boucetta, Hanene

AU - Al-Jamal, Khuloud T.

AU - Müller, Karin H.

AU - Li, Shouping

AU - Porter, Alexandra E.

AU - Eddaoudi, Ayad

AU - Prato, Maurizio

AU - Bianco, Alberto

AU - Kostarelos, Kostas

N1 - Times Cited: 9 0 9

PY - 2011/11/18

Y1 - 2011/11/18

N2 - The impact of nanomaterials such as carbon nanotubes on biological matter is a topic of increasing interest and concern and requires a multifaceted approach to be resolved. A modified cytotoxic (lactate dehydrogenase (LDH)) assay is developed in an attempt to offer a valid and reliable methodology for screening carbon nanotube toxicity in vitro. Two of the most widely used types of surface-modified multiwalled carbon nanotubes (MWNTs) are tested: ammonium-functionalized MWNTs (MWNT-NH 3 +) and Pluronic F127 coated MWNTs (MWNT:F127). Chemically functionalized MWNTs show significantly greater cellular uptake into lung epithelial A549 cells compared to the non-covalently Pluronic F127-coated MWNTs. In spite of this, MWNT:F127 exhibit enhanced cytotoxicity according to the modified LDH assay. The validity of the modified LDH assay is further validated by direct comparison with other less reliable or accurate cytotoxicity assays. These findings indicate the reliability of the modified LDH assay as a screening tool to assess carbon nanotube cytotoxicity and illustrate that high levels of carbon nanotube cellular internalization do not necessarily lead to adverse responses. A modified cytotoxic (LDH) assay is developed in an attempt to offer a valid and reliable methodology for screening carbon nanotube (CNT) toxicity in vitro. Two types of CNT are screened using this assay and the results are compared to other commonly used assays. This assay is a promising tool, particularly in a field eager for reliable in vitro cytotoxicity assays. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

AB - The impact of nanomaterials such as carbon nanotubes on biological matter is a topic of increasing interest and concern and requires a multifaceted approach to be resolved. A modified cytotoxic (lactate dehydrogenase (LDH)) assay is developed in an attempt to offer a valid and reliable methodology for screening carbon nanotube toxicity in vitro. Two of the most widely used types of surface-modified multiwalled carbon nanotubes (MWNTs) are tested: ammonium-functionalized MWNTs (MWNT-NH 3 +) and Pluronic F127 coated MWNTs (MWNT:F127). Chemically functionalized MWNTs show significantly greater cellular uptake into lung epithelial A549 cells compared to the non-covalently Pluronic F127-coated MWNTs. In spite of this, MWNT:F127 exhibit enhanced cytotoxicity according to the modified LDH assay. The validity of the modified LDH assay is further validated by direct comparison with other less reliable or accurate cytotoxicity assays. These findings indicate the reliability of the modified LDH assay as a screening tool to assess carbon nanotube cytotoxicity and illustrate that high levels of carbon nanotube cellular internalization do not necessarily lead to adverse responses. A modified cytotoxic (LDH) assay is developed in an attempt to offer a valid and reliable methodology for screening carbon nanotube (CNT) toxicity in vitro. Two types of CNT are screened using this assay and the results are compared to other commonly used assays. This assay is a promising tool, particularly in a field eager for reliable in vitro cytotoxicity assays. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

KW - cell internalization

KW - cytotoxicity

KW - nanotoxicology

KW - nanotubes

U2 - 10.1002/smll.201101004

DO - 10.1002/smll.201101004

M3 - Article

VL - 7

SP - 3230

EP - 3238

JO - Small

JF - Small

SN - 1613-6810

IS - 22

ER -