Carboxylic acid reductase is a versatile enzyme for the conversion of fatty acids into fuels and chemical commoditiesCitation formats

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Carboxylic acid reductase is a versatile enzyme for the conversion of fatty acids into fuels and chemical commodities. / Kalim Akhtara, M.; Turner, Nicholas J.; Jones, Patrik R.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, No. 1, 02.01.2013, p. 87-92.

Research output: Contribution to journalArticle

Harvard

Kalim Akhtara, M, Turner, NJ & Jones, PR 2013, 'Carboxylic acid reductase is a versatile enzyme for the conversion of fatty acids into fuels and chemical commodities' Proceedings of the National Academy of Sciences of the United States of America, vol. 110, no. 1, pp. 87-92. https://doi.org/10.1073/pnas.1216516110

APA

Kalim Akhtara, M., Turner, N. J., & Jones, P. R. (2013). Carboxylic acid reductase is a versatile enzyme for the conversion of fatty acids into fuels and chemical commodities. Proceedings of the National Academy of Sciences of the United States of America, 110(1), 87-92. https://doi.org/10.1073/pnas.1216516110

Vancouver

Kalim Akhtara M, Turner NJ, Jones PR. Carboxylic acid reductase is a versatile enzyme for the conversion of fatty acids into fuels and chemical commodities. Proceedings of the National Academy of Sciences of the United States of America. 2013 Jan 2;110(1):87-92. https://doi.org/10.1073/pnas.1216516110

Author

Kalim Akhtara, M. ; Turner, Nicholas J. ; Jones, Patrik R. / Carboxylic acid reductase is a versatile enzyme for the conversion of fatty acids into fuels and chemical commodities. In: Proceedings of the National Academy of Sciences of the United States of America. 2013 ; Vol. 110, No. 1. pp. 87-92.

Bibtex

@article{7907bb8caf3643bb9ffb92d1914e3b9f,
title = "Carboxylic acid reductase is a versatile enzyme for the conversion of fatty acids into fuels and chemical commodities",
abstract = "Aliphatic hydrocarbons such as fatty alcohols and petroleum-derived alkanes have numerous applications in the chemical industry. In recent years, the renewable synthesis of aliphatic hydrocarbons has been made possible by engineering microbes to overaccumulate fatty acids. However, to generate end products with the desired physicochemical properties (e.g., fatty aldehydes, alkanes, and alcohols), further conversion of the fatty acid is necessary. A carboxylic acid reductase (CAR) from Mycobacterium marinum was found to convert a wide range of aliphatic fatty acids (C6-C18) into corresponding aldehydes. Together with the broad-substrate specificity of an aldehyde reductase or an aldehyde decarbonylase, the catalytic conversion of fatty acids to fatty alcohols (C8-C16) or fatty alkanes (C7-C15) was reconstituted in vitro. This concept was applied in vivo, in combination with a chain-length-specific thioesterase, to engineer Escherichia coli BL21(DE3) strains that were capable of synthesizing fatty alcohols and alkanes. A fatty alcohol titer exceeding 350 mg•L -1 was obtained in minimal media supplemented with glucose. Moreover, by combining the CAR-dependent pathway with an exogenous fatty acid-generating lipase, natural oils (coconut oil, palm oil, and algal oil bodies) were enzymatically converted into fatty alcohols across a broad chain-length range (C8-C18). Together with complementing enzymes, the broad substrate specificity and kinetic characteristics of CAR opens the road for direct and tailored enzyme-catalyzed conversion of lipids into user-ready chemical commodities.",
keywords = "Biofuel, Green chemistry, Metabolic engineering, Synthetic biology",
author = "{Kalim Akhtara}, M. and Turner, {Nicholas J.} and Jones, {Patrik R.}",
year = "2013",
month = "1",
day = "2",
doi = "10.1073/pnas.1216516110",
language = "English",
volume = "110",
pages = "87--92",
journal = "Proceedings of the National Academy of Sciences",
issn = "0027-8424",
publisher = "National Academy of Sciences",
number = "1",

}

RIS

TY - JOUR

T1 - Carboxylic acid reductase is a versatile enzyme for the conversion of fatty acids into fuels and chemical commodities

AU - Kalim Akhtara, M.

AU - Turner, Nicholas J.

AU - Jones, Patrik R.

PY - 2013/1/2

Y1 - 2013/1/2

N2 - Aliphatic hydrocarbons such as fatty alcohols and petroleum-derived alkanes have numerous applications in the chemical industry. In recent years, the renewable synthesis of aliphatic hydrocarbons has been made possible by engineering microbes to overaccumulate fatty acids. However, to generate end products with the desired physicochemical properties (e.g., fatty aldehydes, alkanes, and alcohols), further conversion of the fatty acid is necessary. A carboxylic acid reductase (CAR) from Mycobacterium marinum was found to convert a wide range of aliphatic fatty acids (C6-C18) into corresponding aldehydes. Together with the broad-substrate specificity of an aldehyde reductase or an aldehyde decarbonylase, the catalytic conversion of fatty acids to fatty alcohols (C8-C16) or fatty alkanes (C7-C15) was reconstituted in vitro. This concept was applied in vivo, in combination with a chain-length-specific thioesterase, to engineer Escherichia coli BL21(DE3) strains that were capable of synthesizing fatty alcohols and alkanes. A fatty alcohol titer exceeding 350 mg•L -1 was obtained in minimal media supplemented with glucose. Moreover, by combining the CAR-dependent pathway with an exogenous fatty acid-generating lipase, natural oils (coconut oil, palm oil, and algal oil bodies) were enzymatically converted into fatty alcohols across a broad chain-length range (C8-C18). Together with complementing enzymes, the broad substrate specificity and kinetic characteristics of CAR opens the road for direct and tailored enzyme-catalyzed conversion of lipids into user-ready chemical commodities.

AB - Aliphatic hydrocarbons such as fatty alcohols and petroleum-derived alkanes have numerous applications in the chemical industry. In recent years, the renewable synthesis of aliphatic hydrocarbons has been made possible by engineering microbes to overaccumulate fatty acids. However, to generate end products with the desired physicochemical properties (e.g., fatty aldehydes, alkanes, and alcohols), further conversion of the fatty acid is necessary. A carboxylic acid reductase (CAR) from Mycobacterium marinum was found to convert a wide range of aliphatic fatty acids (C6-C18) into corresponding aldehydes. Together with the broad-substrate specificity of an aldehyde reductase or an aldehyde decarbonylase, the catalytic conversion of fatty acids to fatty alcohols (C8-C16) or fatty alkanes (C7-C15) was reconstituted in vitro. This concept was applied in vivo, in combination with a chain-length-specific thioesterase, to engineer Escherichia coli BL21(DE3) strains that were capable of synthesizing fatty alcohols and alkanes. A fatty alcohol titer exceeding 350 mg•L -1 was obtained in minimal media supplemented with glucose. Moreover, by combining the CAR-dependent pathway with an exogenous fatty acid-generating lipase, natural oils (coconut oil, palm oil, and algal oil bodies) were enzymatically converted into fatty alcohols across a broad chain-length range (C8-C18). Together with complementing enzymes, the broad substrate specificity and kinetic characteristics of CAR opens the road for direct and tailored enzyme-catalyzed conversion of lipids into user-ready chemical commodities.

KW - Biofuel

KW - Green chemistry

KW - Metabolic engineering

KW - Synthetic biology

U2 - 10.1073/pnas.1216516110

DO - 10.1073/pnas.1216516110

M3 - Article

VL - 110

SP - 87

EP - 92

JO - Proceedings of the National Academy of Sciences

JF - Proceedings of the National Academy of Sciences

SN - 0027-8424

IS - 1

ER -