Calcium dynamics and chromatin remodeling underlie heterogeneity in prolactin transcriptionCitation formats

  • External authors:
  • Claire V Harper
  • Anne Mcnamara
  • Jayne C Charnock
  • Michael White

Standard

Calcium dynamics and chromatin remodeling underlie heterogeneity in prolactin transcription. / Harper, Claire V; Mcnamara, Anne; Spiller, David; Charnock, Jayne C; White, Michael; Davis, Julian.

In: Journal of molecular endocrinology, 05.10.2020.

Research output: Contribution to journalArticlepeer-review

Harvard

Harper, CV, Mcnamara, A, Spiller, D, Charnock, JC, White, M & Davis, J 2020, 'Calcium dynamics and chromatin remodeling underlie heterogeneity in prolactin transcription', Journal of molecular endocrinology.

APA

Harper, C. V., Mcnamara, A., Spiller, D., Charnock, J. C., White, M., & Davis, J. (Accepted/In press). Calcium dynamics and chromatin remodeling underlie heterogeneity in prolactin transcription. Journal of molecular endocrinology.

Vancouver

Harper CV, Mcnamara A, Spiller D, Charnock JC, White M, Davis J. Calcium dynamics and chromatin remodeling underlie heterogeneity in prolactin transcription. Journal of molecular endocrinology. 2020 Oct 5.

Author

Harper, Claire V ; Mcnamara, Anne ; Spiller, David ; Charnock, Jayne C ; White, Michael ; Davis, Julian. / Calcium dynamics and chromatin remodeling underlie heterogeneity in prolactin transcription. In: Journal of molecular endocrinology. 2020.

Bibtex

@article{fc495942208043a495bf89a31b0ae83f,
title = "Calcium dynamics and chromatin remodeling underlie heterogeneity in prolactin transcription",
abstract = "Pituitary cells have been reported to show spontaneous calcium oscillations and dynamic transcription cycles. To study both processes in the same living cell in real-time, we used rat pituitary GH3 cells stably expressing human prolactin-luciferase or prolactin-EGFP reporter gene constructs loaded with a fluorescent calcium indicator and measured activity using single cell time-lapse microscopy. We observed heterogeneity between clonal cells in the calcium activity and prolactin transcription in unstimulated conditions. There was a significant correlation between cells displaying spontaneous calcium spikes and cells showing spontaneous bursts in prolactin expression. Notably, cells showing nobasal calcium activity showed low prolactin expression but elicited a significantly greater transcriptional response to BayK8644 compared to cells showing basal calcium activity. This suggested the presence of two subsets of cells within the population at any one time. Fluorescence-activated cell sorting was used to sort cells into two populations based on the expression level of prolactin-EGFP however, the bimodal pattern of expression was restored within 26h. Chromatin immunoprecipitation showed that these sorted populations were distinct due to the extent of histone acetylation. We suggest that maintenance of a heterogeneous bimodal population is a fundamental characteristic of this cell type and that calcium activation and histone acetylation at least in part, drive prolactin transcriptional competence.",
author = "Harper, {Claire V} and Anne Mcnamara and David Spiller and Charnock, {Jayne C} and Michael White and Julian Davis",
year = "2020",
month = oct,
day = "5",
language = "English",
journal = "Journal of molecular endocrinology",
issn = "0952-5041",
publisher = "Society for Endocrinology",

}

RIS

TY - JOUR

T1 - Calcium dynamics and chromatin remodeling underlie heterogeneity in prolactin transcription

AU - Harper, Claire V

AU - Mcnamara, Anne

AU - Spiller, David

AU - Charnock, Jayne C

AU - White, Michael

AU - Davis, Julian

PY - 2020/10/5

Y1 - 2020/10/5

N2 - Pituitary cells have been reported to show spontaneous calcium oscillations and dynamic transcription cycles. To study both processes in the same living cell in real-time, we used rat pituitary GH3 cells stably expressing human prolactin-luciferase or prolactin-EGFP reporter gene constructs loaded with a fluorescent calcium indicator and measured activity using single cell time-lapse microscopy. We observed heterogeneity between clonal cells in the calcium activity and prolactin transcription in unstimulated conditions. There was a significant correlation between cells displaying spontaneous calcium spikes and cells showing spontaneous bursts in prolactin expression. Notably, cells showing nobasal calcium activity showed low prolactin expression but elicited a significantly greater transcriptional response to BayK8644 compared to cells showing basal calcium activity. This suggested the presence of two subsets of cells within the population at any one time. Fluorescence-activated cell sorting was used to sort cells into two populations based on the expression level of prolactin-EGFP however, the bimodal pattern of expression was restored within 26h. Chromatin immunoprecipitation showed that these sorted populations were distinct due to the extent of histone acetylation. We suggest that maintenance of a heterogeneous bimodal population is a fundamental characteristic of this cell type and that calcium activation and histone acetylation at least in part, drive prolactin transcriptional competence.

AB - Pituitary cells have been reported to show spontaneous calcium oscillations and dynamic transcription cycles. To study both processes in the same living cell in real-time, we used rat pituitary GH3 cells stably expressing human prolactin-luciferase or prolactin-EGFP reporter gene constructs loaded with a fluorescent calcium indicator and measured activity using single cell time-lapse microscopy. We observed heterogeneity between clonal cells in the calcium activity and prolactin transcription in unstimulated conditions. There was a significant correlation between cells displaying spontaneous calcium spikes and cells showing spontaneous bursts in prolactin expression. Notably, cells showing nobasal calcium activity showed low prolactin expression but elicited a significantly greater transcriptional response to BayK8644 compared to cells showing basal calcium activity. This suggested the presence of two subsets of cells within the population at any one time. Fluorescence-activated cell sorting was used to sort cells into two populations based on the expression level of prolactin-EGFP however, the bimodal pattern of expression was restored within 26h. Chromatin immunoprecipitation showed that these sorted populations were distinct due to the extent of histone acetylation. We suggest that maintenance of a heterogeneous bimodal population is a fundamental characteristic of this cell type and that calcium activation and histone acetylation at least in part, drive prolactin transcriptional competence.

M3 - Article

JO - Journal of molecular endocrinology

JF - Journal of molecular endocrinology

SN - 0952-5041

ER -