Array-based diagnostic gene-expression score for atopy and asthmaCitation formats

  • Authors:
  • Martin H. Brutsche
  • Ladina Joos
  • Ingrid E A Carlen Brutsche
  • Roland Bissinger
  • Michael Tamm
  • And 2 others
  • External authors:
  • Adnan Custovic
  • Ashley Woodcock

Standard

Array-based diagnostic gene-expression score for atopy and asthma. / Brutsche, Martin H.; Joos, Ladina; Carlen Brutsche, Ingrid E A; Bissinger, Roland; Tamm, Michael; Custovic, Adnan; Woodcock, Ashley.

In: Journal of Allergy and Clinical Immunology, Vol. 109, No. 2, 2002, p. 271-273.

Research output: Contribution to journalArticle

Harvard

Brutsche, MH, Joos, L, Carlen Brutsche, IEA, Bissinger, R, Tamm, M, Custovic, A & Woodcock, A 2002, 'Array-based diagnostic gene-expression score for atopy and asthma' Journal of Allergy and Clinical Immunology, vol. 109, no. 2, pp. 271-273. https://doi.org/10.1067/mai.2002.121530

APA

Brutsche, M. H., Joos, L., Carlen Brutsche, I. E. A., Bissinger, R., Tamm, M., Custovic, A., & Woodcock, A. (2002). Array-based diagnostic gene-expression score for atopy and asthma. Journal of Allergy and Clinical Immunology, 109(2), 271-273. https://doi.org/10.1067/mai.2002.121530

Vancouver

Brutsche MH, Joos L, Carlen Brutsche IEA, Bissinger R, Tamm M, Custovic A et al. Array-based diagnostic gene-expression score for atopy and asthma. Journal of Allergy and Clinical Immunology. 2002;109(2):271-273. https://doi.org/10.1067/mai.2002.121530

Author

Brutsche, Martin H. ; Joos, Ladina ; Carlen Brutsche, Ingrid E A ; Bissinger, Roland ; Tamm, Michael ; Custovic, Adnan ; Woodcock, Ashley. / Array-based diagnostic gene-expression score for atopy and asthma. In: Journal of Allergy and Clinical Immunology. 2002 ; Vol. 109, No. 2. pp. 271-273.

Bibtex

@article{dae931b5b9464db09370bfa70b73229d,
title = "Array-based diagnostic gene-expression score for atopy and asthma",
abstract = "Whether gene expression is useful in discriminating different atopic phenotypes is unclear. The aim of the study was to evaluate a gene-expression score for the diagnosis of atopy and asthma and to assess disease activity as a guide for therapeutic decisions. Purified mRNA from PBMCs of 18 atopic asthmatic subjects, 8 atopic nonasthmatic subjects, and 14 healthy control subjects was hybridized to cDNA membranes. A composite atopy gene expression (CAGE) score was determined by using 10 genes dysregulated in atopic individuals according to a specific algorithm. The CAGE score was better than total IgE in differentiating atopic from nonatopic subjects (sensitivity, 96{\%}; specificity, 92{\%}). Correlation between the CAGE score and total IgE (P <.001) was found, and there was a trend for correlation with asthma severity (P = .051). The CAGE score was able to quantify phenotype-specific alteration in gene expression of atopic individuals. The CAGE score might be used as a diagnostic tool or to monitor the effects and side effects of therapy.",
keywords = "Asthma, Gene expression, Health-status indicators, Respiratory hypersensitivity",
author = "Brutsche, {Martin H.} and Ladina Joos and {Carlen Brutsche}, {Ingrid E A} and Roland Bissinger and Michael Tamm and Adnan Custovic and Ashley Woodcock",
year = "2002",
doi = "10.1067/mai.2002.121530",
language = "English",
volume = "109",
pages = "271--273",
journal = "Journal of Allergy and Clinical Immunology",
issn = "0091-6749",
publisher = "Mosby, Inc.",
number = "2",

}

RIS

TY - JOUR

T1 - Array-based diagnostic gene-expression score for atopy and asthma

AU - Brutsche, Martin H.

AU - Joos, Ladina

AU - Carlen Brutsche, Ingrid E A

AU - Bissinger, Roland

AU - Tamm, Michael

AU - Custovic, Adnan

AU - Woodcock, Ashley

PY - 2002

Y1 - 2002

N2 - Whether gene expression is useful in discriminating different atopic phenotypes is unclear. The aim of the study was to evaluate a gene-expression score for the diagnosis of atopy and asthma and to assess disease activity as a guide for therapeutic decisions. Purified mRNA from PBMCs of 18 atopic asthmatic subjects, 8 atopic nonasthmatic subjects, and 14 healthy control subjects was hybridized to cDNA membranes. A composite atopy gene expression (CAGE) score was determined by using 10 genes dysregulated in atopic individuals according to a specific algorithm. The CAGE score was better than total IgE in differentiating atopic from nonatopic subjects (sensitivity, 96%; specificity, 92%). Correlation between the CAGE score and total IgE (P <.001) was found, and there was a trend for correlation with asthma severity (P = .051). The CAGE score was able to quantify phenotype-specific alteration in gene expression of atopic individuals. The CAGE score might be used as a diagnostic tool or to monitor the effects and side effects of therapy.

AB - Whether gene expression is useful in discriminating different atopic phenotypes is unclear. The aim of the study was to evaluate a gene-expression score for the diagnosis of atopy and asthma and to assess disease activity as a guide for therapeutic decisions. Purified mRNA from PBMCs of 18 atopic asthmatic subjects, 8 atopic nonasthmatic subjects, and 14 healthy control subjects was hybridized to cDNA membranes. A composite atopy gene expression (CAGE) score was determined by using 10 genes dysregulated in atopic individuals according to a specific algorithm. The CAGE score was better than total IgE in differentiating atopic from nonatopic subjects (sensitivity, 96%; specificity, 92%). Correlation between the CAGE score and total IgE (P <.001) was found, and there was a trend for correlation with asthma severity (P = .051). The CAGE score was able to quantify phenotype-specific alteration in gene expression of atopic individuals. The CAGE score might be used as a diagnostic tool or to monitor the effects and side effects of therapy.

KW - Asthma

KW - Gene expression

KW - Health-status indicators

KW - Respiratory hypersensitivity

U2 - 10.1067/mai.2002.121530

DO - 10.1067/mai.2002.121530

M3 - Article

VL - 109

SP - 271

EP - 273

JO - Journal of Allergy and Clinical Immunology

JF - Journal of Allergy and Clinical Immunology

SN - 0091-6749

IS - 2

ER -