Arginase-1 is expressed exclusively by infiltrating myeloid cells in CNS injury and disease

Research output: Contribution to journalArticlepeer-review

  • External authors:
  • Rosmarini Passos Dos Santos
  • Juan Guillermo Zarruk
  • Christopher K. Salmon
  • Antje Kroner
  • Samuel David

Abstract

Resident microglia and infiltrating myeloid cells play important roles in the onset, propagation, and resolution of inflammation in central nervous system (CNS) injury and disease. Identifying cell type-specific mechanisms will help to appropriately target interventions for tissue repair. Arginase-1 (Arg-1) is a well characterised modulator of tissue repair and its expression correlates with recovery after CNS injury. Here we assessed the cellular localisation of Arg-1 in two models of CNS damage. Using microglia specific antibodies, P2ry12 and Fc receptor-like S (FCRLS), we show the LysM-EGFP reporter mouse is an excellent model to distinguish infiltrating myeloid cells from resident microglia. We show that Arg-1 is expressed exclusively in infiltrating myeloid cells but not microglia in models of spinal cord injury (SCI) and experimental autoimmune encephalomyelitis (EAE). Our in vitro studies suggest that factors in the CNS environment prevent expression of Arg-1 in microglia in vivo. This work suggests different functional roles for these cells in CNS injury and repair and shows that such repair pathways can be switched on in infiltrating myeloid cells in pro-inflammatory environments.

Bibliographical metadata

Original languageEnglish
Pages (from-to)61-67
JournalBrain, Behavior, and Immunity
Volume56
Early online date25 Apr 2016
DOIs
Publication statusPublished - 1 Aug 2016

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