Analysis of human total antibody repertoires in TIF1γ autoantibody positive dermatomyositisCitation formats

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@article{83543f2397b04743b8cceea39b7e086c,
title = "Analysis of human total antibody repertoires in TIF1γ autoantibody positive dermatomyositis",
abstract = "We investigate the accumulated microbial and autoantigen antibody repertoire in adult-onset dermatomyositis patients sero-positive for TIF1γ (TRIM33) autoantibodies. We use an untargeted high-throughput approach which combines immunoglobulin disease-specific epitope-enrichment and identification of microbial and human antigens. We observe antibodies recognizing a wider repertoire of microbial antigens in dermatomyositis. Antibodies recognizing viruses and Poxviridae family species are significantly enriched. The identified autoantibodies recognise a large portion of the human proteome, including interferon regulated proteins; these proteins cluster in specific biological processes. In addition to TRIM33, we identify autoantibodies against eleven further TRIM proteins, including TRIM21. Some of these TRIM proteins share epitope homology with specific viral species including poxviruses. Our data suggest antibody accumulation in dermatomyositis against an expanded diversity of microbial and human proteins and evidence of non-random targeting of specific signalling pathways. Our findings indicate that molecular mimicry and epitope spreading events may play a role in dermatomyositis pathogenesis.",
author = "Spyridon Megremis and Thomas Walker and Xiaotong He and James O'Sullivan and William Ollier and Hector Chinoy and Neil Pendleton and Antony Payton and Lynne Hampson and Ian Hampson and Janine Lamb",
year = "2021",
month = mar,
day = "26",
doi = "https://doi.org/10.1038/s42003-021-01932-6",
language = "English",
journal = "Communications Biology",
issn = "2399-3642",
publisher = "Springer Nature",

}

RIS

TY - JOUR

T1 - Analysis of human total antibody repertoires in TIF1γ autoantibody positive dermatomyositis

AU - Megremis, Spyridon

AU - Walker, Thomas

AU - He, Xiaotong

AU - O'Sullivan, James

AU - Ollier, William

AU - Chinoy, Hector

AU - Pendleton, Neil

AU - Payton, Antony

AU - Hampson, Lynne

AU - Hampson, Ian

AU - Lamb, Janine

PY - 2021/3/26

Y1 - 2021/3/26

N2 - We investigate the accumulated microbial and autoantigen antibody repertoire in adult-onset dermatomyositis patients sero-positive for TIF1γ (TRIM33) autoantibodies. We use an untargeted high-throughput approach which combines immunoglobulin disease-specific epitope-enrichment and identification of microbial and human antigens. We observe antibodies recognizing a wider repertoire of microbial antigens in dermatomyositis. Antibodies recognizing viruses and Poxviridae family species are significantly enriched. The identified autoantibodies recognise a large portion of the human proteome, including interferon regulated proteins; these proteins cluster in specific biological processes. In addition to TRIM33, we identify autoantibodies against eleven further TRIM proteins, including TRIM21. Some of these TRIM proteins share epitope homology with specific viral species including poxviruses. Our data suggest antibody accumulation in dermatomyositis against an expanded diversity of microbial and human proteins and evidence of non-random targeting of specific signalling pathways. Our findings indicate that molecular mimicry and epitope spreading events may play a role in dermatomyositis pathogenesis.

AB - We investigate the accumulated microbial and autoantigen antibody repertoire in adult-onset dermatomyositis patients sero-positive for TIF1γ (TRIM33) autoantibodies. We use an untargeted high-throughput approach which combines immunoglobulin disease-specific epitope-enrichment and identification of microbial and human antigens. We observe antibodies recognizing a wider repertoire of microbial antigens in dermatomyositis. Antibodies recognizing viruses and Poxviridae family species are significantly enriched. The identified autoantibodies recognise a large portion of the human proteome, including interferon regulated proteins; these proteins cluster in specific biological processes. In addition to TRIM33, we identify autoantibodies against eleven further TRIM proteins, including TRIM21. Some of these TRIM proteins share epitope homology with specific viral species including poxviruses. Our data suggest antibody accumulation in dermatomyositis against an expanded diversity of microbial and human proteins and evidence of non-random targeting of specific signalling pathways. Our findings indicate that molecular mimicry and epitope spreading events may play a role in dermatomyositis pathogenesis.

U2 - https://doi.org/10.1038/s42003-021-01932-6

DO - https://doi.org/10.1038/s42003-021-01932-6

M3 - Article

JO - Communications Biology

JF - Communications Biology

SN - 2399-3642

ER -