An extracellular matrix fragment drives epithelial remodeling and airway hyperresponsiveness

Research output: Contribution to journalArticle

  • External authors:
  • Dhiren F. Patel
  • Teresa Peiró
  • Amelia Shoemark
  • Samia Akthar
  • Simone A. Walker
  • Aleksander M. Grabiec
  • Patricia L. Jackson
  • Amit Gaggar
  • Xin Xu
  • Jennifer L. Trevor
  • Jindong Li
  • Chad Steele
  • Gael Tavernier
  • J. Edwin Blalock
  • Lisa G. Gregory
  • Clare M. Lloyd
  • Robert J. Snelgrove

Abstract

It is anticipated that bioactive fragments of the extracellular matrix (matrikines) can influence the development and progression of chronic diseases. The enzyme leukotriene A4 hydrolase (LTA4H) mediates opposing proinflammatory and anti-inflammatory activities, through the generation of leukotriene B4 (LTB4) and degradation of pro-neutrophilic matrikine Pro-Gly-Pro (PGP), respectively. We show that abrogation of LTB4 signaling ameliorated inflammation and airway hyperresponsiveness (AHR) in a murine asthma model, yet global loss of LTA4H exacerbated AHR, despite the absence of LTB4. This exacerbated AHR was attributable to a neutrophil-independent capacity of PGP to promote pathological airway epithelial remodeling. Thus, we demonstrate a disconnect between airway inflammation and AHR and the ability of a matrikine to promote an epithelial remodeling phenotype that negatively affects lung function. Subsequently, we show that substantial quantities of PGP are detectable in the sputum of moderate-severe asthmatics in two distinct cohorts of patients. These studies have implications for our understanding of remodeling phenotypes in asthma and may rationalize the failure of LTA4H inhibitors in the clinic.

Bibliographical metadata

Original languageEnglish
Article numbereaaq0693
JournalScience Translational Medicine
Volume10
Issue number455
Early online date22 Aug 2018
DOIs
Publication statusPublished - 2018

Related information

Researchers

View all