An antiinflammatory role for IKKβ through the inhibition of "classical" macrophage activation

Research output: Contribution to journalArticle

  • External authors:
  • Carol Ho Yan Fong
  • Magali Bebien
  • Arnaud Didierlaurent
  • Ruth Nebauer
  • David Broide
  • Michael Karin
  • Toby Lawrence

Abstract

The nuclear factor κB (NF-κB) pathway plays a central role in inflammation and immunity. In response to proinflammatory cytokines and pathogen-associated molecular patterns, NF-κB activation is controlled by IκB kinase (IKK)β. Using Cre/lox -mediated gene targeting of IKKβ, we have uncovered a tissue-specific role for IKKβ during infection with group B streptococcus. Although deletion of IKKβ in airway epithelial cells had the predicted effect of inhibiting inflammation and reducing innate immunity, deletion of IKKβ in the myeloid lineage unexpectedly conferred resistance to infection that was associated with increased expression of interleukin (IL)-12, inducible nitric oxide synthase (NOS2), and major histocompatibility complex (MHC) class II by macrophages. We also describe a previously unknown role for IKKβ in the inhibition of signal transducer and activator of transcription (Stat)1 signaling in macrophages, which is critical for IL-12, NOS2, and MHC class II expression. These studies suggest that IKKβ inhibits the "classically" activated or M1 macrophage phenotype during infection through negative cross talk with the Stat1 pathway. This may represent a mechanism to prevent the over-exuberant activation of macrophages during infection and contribute to the resolution of inflammation. This establishes a new role for IKKβ in the regulation of macrophage activation with important implications in chronic inflammatory disease, infection, and cancer. © 2008 Fong et al. The Rockefeller University Press.

Bibliographical metadata

Original languageEnglish
Pages (from-to)1269-1276
Number of pages7
JournalJournal of Experimental Medicine
Volume205
Issue number6
DOIs
Publication statusPublished - 9 Jun 2008